Yıl: 2020 Cilt: 13 Sayı: 1 Sayfa Aralığı: 20 - 23 Metin Dili: İngilizce İndeks Tarihi: 21-10-2020

Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses

Öz:
The purpose of this research was to evaluate the effects of methimazole used to create hypothyroidism in rats on the eosinophilic cellsand uterus layer thicknesses. Uterus samples, 15 healthy Wistar Albino female rats aged 12-14 weeks were taken from control (C, n=6)and experimental (E, n=9) groups. Intraperitoneally, methimazole was injected as 10 mg/kg/day dose in the Group E for 2 weeks. Afterhypothyroidism was created in the Group E, feeding all the rats in this study was continued with normal pellet feed again for 2 weeks tosee chronic effects on tissue. At the end of the 4th week, uterus samples were stained with Crossmon’s trichrome staining. Uterus layerthicknesses were measuring with ImageJ Analysis using images of six different uterus regions including endometrium, myometrium andperimetrium layers. The mean number of eosinophil granulocyte in the endometrium was higher in Group E when compared to Group C(P<0.05). Its distribution was concentrated in the endometrium functionalis. However, there was no difference in uterine layerthicknesses between the groups (P>0.05). We concluded that methimazole might cause hypereosinophil granulocyte accumulation inthe endometrium functionalis. This study is important for clinicians to evaluate methimazole-associated eosinophilic infiltration due tohypersensitive immune reactions to the drugs, especially used during pregnancy.
Anahtar Kelime:

Metimazolün Uterus Katman Kalınlıklarına ve Eozinofil Granülosit Değişimlerine Etkisi

Öz:
Bu çalışmanın amacı ratlarda hipotiroidizm oluşturmada kullanılan metimazolün uterus katman kalınlıklarına ve eozinofil granülositler üzerindeki etkilerini değerlendirmektedir. Çalışmanın uterus örnekleri kontrol (K, n=6) ve deney (D, n=9) gruplarını oluşturan 12-14 haftalık 15 sağlıklı dişi Wistar Albino rattan alındı. Deney grubuna 2 hafta boyunca intraperitoneyal olarak 10 mg/kg/gün dozunda metimazol enjekte edildi. Deney grubunda hipotiroidizm oluşturulduktan sonra doku üzerindeki kronik etkileri görmek için çalışmadaki tüm ratların beslenmesine 2 hafta daha normal pelet yemle devam edildi. 4. haftanın sonunda alınan uterus örnekleri Crossmon’s trikrom boyamasıyla boyandı. Endometriyum, miyometriyum ve perimetriyum katmanlarını içeren 6 farklı uterus görüntüsünün uterus katman kalınlıkları ImageJ analiz programı kullanılarak ölçüldü. Endometriyumdaki ortalama eozinofil granülosit sayısı kontrol grubuna kıyasla deney grubunda daha yüksekti (P<0.05). Bu dağılım endometriyum fonksiyonalis katında yoğunlaşmıştı. Fakat gruplar arasındaki uterus katman kalınlıklarında farklılık yoktu. Metimazolün endometriyum fonksiyonaliste hipereozinofilik granülosit birikimine sebep olabildiği sonucuna varıldı. Bu çalışma özellikle gebelik döneminde kullanılan ilaçların hipersensitif immün reaksiyonlara bağlı metimazol ilişkili eozinofilik hücre infiltrasyonunu değerlendirmede klinisyenler için önemlidir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA COLAKOGLU F, Selçuk M, DÖNMEZ H (2020). Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. , 20 - 23.
Chicago COLAKOGLU Fatma,Selçuk Muhammet Lütfi,DÖNMEZ HASAN HÜSEYİN Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. (2020): 20 - 23.
MLA COLAKOGLU Fatma,Selçuk Muhammet Lütfi,DÖNMEZ HASAN HÜSEYİN Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. , 2020, ss.20 - 23.
AMA COLAKOGLU F,Selçuk M,DÖNMEZ H Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. . 2020; 20 - 23.
Vancouver COLAKOGLU F,Selçuk M,DÖNMEZ H Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. . 2020; 20 - 23.
IEEE COLAKOGLU F,Selçuk M,DÖNMEZ H "Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses." , ss.20 - 23, 2020.
ISNAD COLAKOGLU, Fatma vd. "Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses". (2020), 20-23.
APA COLAKOGLU F, Selçuk M, DÖNMEZ H (2020). Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(1), 20 - 23.
Chicago COLAKOGLU Fatma,Selçuk Muhammet Lütfi,DÖNMEZ HASAN HÜSEYİN Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13, no.1 (2020): 20 - 23.
MLA COLAKOGLU Fatma,Selçuk Muhammet Lütfi,DÖNMEZ HASAN HÜSEYİN Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol.13, no.1, 2020, ss.20 - 23.
AMA COLAKOGLU F,Selçuk M,DÖNMEZ H Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniversitesi Veteriner Fakültesi Dergisi. 2020; 13(1): 20 - 23.
Vancouver COLAKOGLU F,Selçuk M,DÖNMEZ H Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses. Dicle Üniversitesi Veteriner Fakültesi Dergisi. 2020; 13(1): 20 - 23.
IEEE COLAKOGLU F,Selçuk M,DÖNMEZ H "Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses." Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13, ss.20 - 23, 2020.
ISNAD COLAKOGLU, Fatma vd. "Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses". Dicle Üniversitesi Veteriner Fakültesi Dergisi 13/1 (2020), 20-23.