The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines
Yıl: 2020 Cilt: 26 Sayı: 5 Sayfa Aralığı: 697 - 704 Metin Dili: İngilizce DOI: 10.9775/kvfd.2020.24286 İndeks Tarihi: 16-10-2020
The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines
Öz: This study aims to explore the pharmacodynamics of chelidonine, the active ingredient in greater celandine (Chelidonium majus L.), on in vitro ratbladder and trachea tissue, and evaluate its cell protective effects on primary lung and kidney cell lines. The study was carried out via repeatedapplications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca++ in a calcium-free environment, on urinary bladder tissue, andrepeated applications of acetylcholine, atropine, carbachol and mecamylamine on trachea tissue. At the same time, cell viability and catalaseand superoxide dismutase activity was measured on primary cell lines obtained from lung and kidney tissue samples. The study has shown thatchelidonine has a relaxant effect on bladder and trachea tissues, and it may be mentioned that this effect is produced via muscarinic receptors. Inaddition, chelidonin caused a statistically insignificant increase in cell viability in primary lung and kidney cell lines at increasing doses (1 and 4 μg/mL), but this increase remained at the control group level. In contrast, chelidonin caused a significant decrease in cell viability at the same cell linesat doses of 8 and 16 μg/mL. In conclusion, it is suggested that greater celandine, which is used in folk medicine, and its active ingredient chelidoninemight have beneficial effects on asthma, urinary incontinence and other urinary tract and respiratory diseases among others.
Anahtar Kelime: Kırlangıç Otu Etkin Maddesi Chelidoninin İzole Rat İdrar Kesesi ve Trakea Düz Kasları ve Primer Akciğer ve Böbrek Hücre Hatları Üzerine Etkisi
Öz: Bu çalışmada, kırlangıç otu (Chelidonium majus L.) etkin maddesi chelidoninin in vitro rat idrar kesesi ve trakea dokusu üzerindeki farmakodinamik etkilerinin değerlendirilmesi ve primer akciğer ve böbrek hücre hatları üzerine hücre koruyucu etkilerinin araştırılması amaçlandı. Çalışma, idrar kesesi dokusunda tekrarlı yapılan asetilkolin, atropin, verapamil ve oksibutinin ile kalsiyumsuz ortamda Ca++ uygulamalarıyla, trakea dokusuna ise tekrarlı uygulamalarla asetilkolin, atropin, karbakol ve mekamilamin verilerek gerçekleştirildi. Aynı zamanda, alınan akciğer ve böbrek dokularından elde edilen primer hücre hatları üzerinde hücre canlılığı ölçümleri, katalaz ve süperoksit dismutaz enzim aktivitelerinin tayini yapıldı. Çalışma sonucunda, kırlangıç otu etkin maddesi chelidoninin idrar kesesi ve trakea dokuları üzerinde gevşetici etkisinin olduğu belirlendi ve chelidoninin bu etkisini muskarinik reseptörler üzerinden gösterdiğinden söz edilebilir. Ayrıca, chelidonin artan dozlarda (1 ve 4 μg/mL) primer akciğer ve böbrek hücre hatlarında hücre canlılığında istatistiksel olarak önemsiz bir artışa neden oldu, ancak bu artış kontrol grubu seviyesinde kaldı. Buna karşılık chelidonin aynı hücre hatlarında 8 ve 16 μg/mL dozlarında hücre canlılığında önemli bir düşüşe neden oldu. Sonuç olarak, halk arasında tıbbi amaçlarla kullanılan kırlangıç otunun ve etkin maddesi chelidonin astım, üriner inkontinans, idrar yolları ve solunum sistemi hastalıkları vb. durumlarda faydalı etkileri olabileceği görüşüne varıldı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ARSLANBAS E, KARA H, Turgut N, GUNGOR H, DOĞAN H, ATASOY M, KUMRU A (2020). The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. , 697 - 704. 10.9775/kvfd.2020.24286 |
| Chicago | ARSLANBAS EMRE,KARA Haki,Turgut Nergiz,GUNGOR HUSEYIN,DOĞAN Halef Okan,ATASOY Mustafa Ozan,KUMRU Alper Serhat The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. (2020): 697 - 704. 10.9775/kvfd.2020.24286 |
| MLA | ARSLANBAS EMRE,KARA Haki,Turgut Nergiz,GUNGOR HUSEYIN,DOĞAN Halef Okan,ATASOY Mustafa Ozan,KUMRU Alper Serhat The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. , 2020, ss.697 - 704. 10.9775/kvfd.2020.24286 |
| AMA | ARSLANBAS E,KARA H,Turgut N,GUNGOR H,DOĞAN H,ATASOY M,KUMRU A The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. . 2020; 697 - 704. 10.9775/kvfd.2020.24286 |
| Vancouver | ARSLANBAS E,KARA H,Turgut N,GUNGOR H,DOĞAN H,ATASOY M,KUMRU A The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. . 2020; 697 - 704. 10.9775/kvfd.2020.24286 |
| IEEE | ARSLANBAS E,KARA H,Turgut N,GUNGOR H,DOĞAN H,ATASOY M,KUMRU A "The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines." , ss.697 - 704, 2020. 10.9775/kvfd.2020.24286 |
| ISNAD | ARSLANBAS, EMRE vd. "The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines". (2020), 697-704. https://doi.org/10.9775/kvfd.2020.24286 |
| APA | ARSLANBAS E, KARA H, Turgut N, GUNGOR H, DOĞAN H, ATASOY M, KUMRU A (2020). The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(5), 697 - 704. 10.9775/kvfd.2020.24286 |
| Chicago | ARSLANBAS EMRE,KARA Haki,Turgut Nergiz,GUNGOR HUSEYIN,DOĞAN Halef Okan,ATASOY Mustafa Ozan,KUMRU Alper Serhat The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26, no.5 (2020): 697 - 704. 10.9775/kvfd.2020.24286 |
| MLA | ARSLANBAS EMRE,KARA Haki,Turgut Nergiz,GUNGOR HUSEYIN,DOĞAN Halef Okan,ATASOY Mustafa Ozan,KUMRU Alper Serhat The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.26, no.5, 2020, ss.697 - 704. 10.9775/kvfd.2020.24286 |
| AMA | ARSLANBAS E,KARA H,Turgut N,GUNGOR H,DOĞAN H,ATASOY M,KUMRU A The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(5): 697 - 704. 10.9775/kvfd.2020.24286 |
| Vancouver | ARSLANBAS E,KARA H,Turgut N,GUNGOR H,DOĞAN H,ATASOY M,KUMRU A The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(5): 697 - 704. 10.9775/kvfd.2020.24286 |
| IEEE | ARSLANBAS E,KARA H,Turgut N,GUNGOR H,DOĞAN H,ATASOY M,KUMRU A "The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26, ss.697 - 704, 2020. 10.9775/kvfd.2020.24286 |
| ISNAD | ARSLANBAS, EMRE vd. "The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26/5 (2020), 697-704. https://doi.org/10.9775/kvfd.2020.24286 |
