Antiproliferative Effects of Carvacrol on Neuroblastoma Cells
Yıl: 2020 Cilt: 10 Sayı: 1 Sayfa Aralığı: 61 - 64 Metin Dili: İngilizce DOI: 10.5222/buchd.2020.59251 İndeks Tarihi: 17-10-2020
Antiproliferative Effects of Carvacrol on Neuroblastoma Cells
Öz: Objective: Neuroblastoma is a pediatric solid tumor that originates from the neural crest. The survival rate of these malignancies is below 50 %. The most common treatments for the disease have several adverse effects which make them invasive for patients and limits the applicability as well as continuity of the treatment. Therefore, researchers seek new agents to reduce adverse effects in order to improve the treatment. In this wise, plant-derived phytochemicals are promising drug candidates, such as carvacrol which has anti-cancer features in some cancers.Method: Human neuroblastoma cell lines, KELLY (N-MYC positive) and SH-SY5Y (N-MYC negative) were grown in RPMI 1640 (KELLY) or DMEM (SH-SY5Y). Cells were treated with a range of concentration of carvacrol. Cellular proliferation was monitored by using xCELLigence Real-Time Cell Analyzer (RTCA) (ACEABiosciences, Inc.) device to evaluate the antiproliferative effect of carvacrol on the neuroblastoma cells.Results: Considering the proliferation curve of SH-SY5Y, results indicate that carvacrol has antiproliferative effects on N-MYC negative neuroblastoma cells, SH-SY5Y cells at all concentrations.Conclusion: The data illuminate that the carvacrol has an antiproliferative role on neuroblastoma cells. In this context, this phyto-compound might be a promising agent for the treatment of this malignancy
Anahtar Kelime: Karvakrolün Nöroblastom Hücreleri Üzerindeki Antiproliferatif Etkisi
Öz: Amaç: Nöroblastom, nöral krest kaynaklı pediyatrik bir solid tümördür. Bu malignitelerin hayatta kalma oranı %50’nin altındadır. Hastalık için en yaygın tedavilerin, hastalar için invaziv olmasını sağlayan ve tedavinin uygulanabilirliğini ve sürekliliğini sınırlayan çeşitli olumsuz etkileri vardır. Bu nedenle, araştırmacılar tedaviyi iyileştirmek ve olumsuz etkileri azaltmak için yeni ajanlar aramaktalar. Bu açıdan bitki kaynaklı fitokimyasallar, bazı kanserlerde anti-kanser özelliklerine sahip olan karvakrol gibi umut verici ilaç adaylarıdır. Yöntem: İnsan nöroblastom hücre dizileri, KELLY (N-MYC pozitif) ve SH-SY5Y (N-MYC negatif) RPMI 1640 (KELLY) veya DMEM’de (SH-SY5Y) büyütüldü. Hücreler bir dizi karvakrol konsantrasyonu ile muamele edildi. Hücresel proliferasyon, karvakrolün nöroblastom hücreleri üzerindeki anti-proliferatif etkisini değerlendirmek için xCELLigence Gerçek Zamanlı Hücre Analiz Cihazı (RTCA) (ACEA Biosciences, Inc.) cihazı kullanıldı. Bulgular: SH-SY5Y’nin proliferasyon eğrisi göz önüne alındığında, sonuçlar karvakrolün tüm konsantrasyonlarda N-MYC negatif nöroblastom hücreleri SH-SY5Y hücreleri üzerinde anti-proliferatif etkilere sahip olduğunu göstermektedir. Sonuç: Veriler, karvakrolün nöroblastom hücreleri üzerinde anti-proliferatif bir role sahip olduğunu aydınlatmaktadır. Bu bağlamda, bu fitokimyasal malignite için umut verici bir ajan olabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Calibasi Kocal G, Pakdemirli A (2020). Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. , 61 - 64. 10.5222/buchd.2020.59251 |
| Chicago | Calibasi Kocal Gizem,Pakdemirli Ahu Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. (2020): 61 - 64. 10.5222/buchd.2020.59251 |
| MLA | Calibasi Kocal Gizem,Pakdemirli Ahu Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. , 2020, ss.61 - 64. 10.5222/buchd.2020.59251 |
| AMA | Calibasi Kocal G,Pakdemirli A Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. . 2020; 61 - 64. 10.5222/buchd.2020.59251 |
| Vancouver | Calibasi Kocal G,Pakdemirli A Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. . 2020; 61 - 64. 10.5222/buchd.2020.59251 |
| IEEE | Calibasi Kocal G,Pakdemirli A "Antiproliferative Effects of Carvacrol on Neuroblastoma Cells." , ss.61 - 64, 2020. 10.5222/buchd.2020.59251 |
| ISNAD | Calibasi Kocal, Gizem - Pakdemirli, Ahu. "Antiproliferative Effects of Carvacrol on Neuroblastoma Cells". (2020), 61-64. https://doi.org/10.5222/buchd.2020.59251 |
| APA | Calibasi Kocal G, Pakdemirli A (2020). Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi, 10(1), 61 - 64. 10.5222/buchd.2020.59251 |
| Chicago | Calibasi Kocal Gizem,Pakdemirli Ahu Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi 10, no.1 (2020): 61 - 64. 10.5222/buchd.2020.59251 |
| MLA | Calibasi Kocal Gizem,Pakdemirli Ahu Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi, vol.10, no.1, 2020, ss.61 - 64. 10.5222/buchd.2020.59251 |
| AMA | Calibasi Kocal G,Pakdemirli A Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi. 2020; 10(1): 61 - 64. 10.5222/buchd.2020.59251 |
| Vancouver | Calibasi Kocal G,Pakdemirli A Antiproliferative Effects of Carvacrol on Neuroblastoma Cells. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi. 2020; 10(1): 61 - 64. 10.5222/buchd.2020.59251 |
| IEEE | Calibasi Kocal G,Pakdemirli A "Antiproliferative Effects of Carvacrol on Neuroblastoma Cells." İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi, 10, ss.61 - 64, 2020. 10.5222/buchd.2020.59251 |
| ISNAD | Calibasi Kocal, Gizem - Pakdemirli, Ahu. "Antiproliferative Effects of Carvacrol on Neuroblastoma Cells". İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi 10/1 (2020), 61-64. https://doi.org/10.5222/buchd.2020.59251 |
