Objective: ABO blood type has been reported to be associated with various types of cancer such as lung cancer, prostate cancer, bladder cancer andpancreatic adenocarcinoma. We aimed to determine whether there is a relationship between ABO blood subtypes and testicular cancer incidence inTurkish population.Materials and Methods: We retrospectively analyzed the medical records of all patients who underwent inguinal orchiectomy in our departmentbetween 2008 and 2018.Results: A total of 138 patients were included in the study. The age of the patients ranged from two to 67 years, with a mean age of 32.9±10.9 years.Of the 138 participants, 45 (32.6%) had blood type A, 27 (19.6%) had blood type B, 50 had blood type O (36.2%) and 16 (11.6%) had blood typeAB. One hundred and twenty-five patients (90.6%) were Rhesus (+) and 13 patients (9.4%) were Rhesus (-). There was no statistically significantdifference between ABO blood subtypes according to testicular pathology (p=0.713). Interestingly, the percentage of patients with AB blood subtypewas the lowest.Conclusion: These results suggest that Turkish men with O blood subtype and Rh (+) are prone to develop testicular cancer. However, these findingsshould be supported by further studies conducted with a large number of participants.