Introduction: The aim of this study was to point the clinical features of patients with central line associated bloodstream infection (CLABSI) and evaluation of mortality related risk factors. Matherial and Method: The study performed retrospectively, with patients over 18, who were diagnosed with CLABSI, between 2011 and 2018 in intensive care units (ICU) of a training and research hospital, to evaluate the clinical features and 28 day survival/mortality associated risk factors. Results: In this study, a total of 143 episodes of CLABSIs were identified among 98949 hospitalization and 60566 catheter days. CLABSI rate was 1.598-4.955 per 1000 catheter days from 2011 to 2018. The patients were followed in primary, secondary and tertiary level ICUs; 48 (33.6%), 34 (23.8%), 32 (22.4%) respectively. In addition, 29 (20.2%) of them hospitalized in burn intensive care unit. Mechanical ventilatory support was needed for 107 of the patients. And the mean APACHE II score was 25.18±6.08 (7-39) on admission to ICU. Of the patients who were diagnosed with CLABSI, 59 (41.3%) survived while 84 (58.7%) died on the 28th day. Univariate analysis showed that the age (p: 0.000), the condition of congestive heart failure (p: 0.038) and Pitt bacteremia score (p: 0.006) were significant risk factors for mortality. According to multivariate analysis, only age (p: 0.003) and Pitt bacteremia score (p: 0.028) were significantly higher. Of the isolated microorganisms 42.6% were Gram negative, followed by Gram positive (28.7%) and Candida spp (28.7%). There was no significant correlation between microorganism type and survival. The most common pathogens were coagulase-negative staphylococci (23/143, %16.1), K.pneumoniae (22/143, %15.4), A.baumannii (18/143, %12.6) and C.parapsilosis (17/143, %11.9). Conclusion: CLABSIs are important infections and associated with increased mortality when in common with older age or severe illness. For the follow up of those patients, clinicians can use the Pitt bacteremia score which is easy to use.