Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line

Yıl: 2020 Cilt: 11 Sayı: 4 Sayfa Aralığı: 607 - 612 Metin Dili: İngilizce DOI: 10.31067/0.2019.194 İndeks Tarihi: 19-10-2020

Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line

Öz:
Objectives: The accumulation of free fatty acids in non-adipose tissues lead to cellular lipotoxicity and induce endoplasmic reticulum (ER) stress. To restore ER homeostasis, cells evoke an adaptive mechanism that is known as the unfolded protein response (UPR). The aim of this study was to investigate the potential relationship between ER stress and some inflammasome complexes under lipotoxic ER stress conditions in the mouse macrophage cell line (RAW 264.7).Materials and Methods: The mouse macrophage cells (RAW 264.7) were treated with Ethanol-BSA (control) or Palmitate-BSA (500μM). The expression of inflammation-related target genes was investigated using the qRTPCR method.Results: A significant induction at the mRNA levels of mTNFα, mNFκBIB, mIRF7, mCCL5, and reduction at the mRNA level of mIRF1 after lipid-induced metabolic ER stress were observed in RAW 264.7 cells.Conclusion: Together, these findings confirm that UPR regulates the expression of key inflammation-related genes under saturated lipid-induced stress conditions.
Anahtar Kelime:

LİPOTOKSİK ENDOPLAZMIK STRES SONUCU İNFLAMASYON ILE İLIŞKILI BAZI GEN İFADELERININ FARE MAKROFAJ HÜCRE HATTINDA ARAŞTIRILMASI

Öz:
Amaç: Adipoz olmayan dokularda serbest yağ asitlerinin birikmesi, hücresel lipotoksisiteye ve endoplazmik retikulum (ER) stresine neden olur. ER homeostazını geri kazanmak için hücreler, katlanmamış protein tepkisi (UPR) olarak bilinen bir uyarlamalı mekanizma uyandırır. Bu çalışmada, lipotoksik ER stres koşulları altında ER stres ve bazı inflamasyon kompleksleri arasındaki moleküler ilişki fare makrofaj hücre hattında (RAW 264.7) araştırılmıştır.Gereç ve Yöntem: Fare makrofaj hücreleri (RAW 264.7), Ethanol-BSA (kontrol) veya Palmitate-BSA (500 μM) ile muamele edildi. İnflamasyonla ilişkili hedef genlerin ekspresyon değişimleri qRT-PCR yöntemi kullanılarak araştırıldı.Bulgular: RAW 264.7 hücrelerinde lipit kaynaklı metabolik ER stres sonrasında; mTNFα, mNFκBIB, mIRF7, mCCL5 mRNA seviyelerinde artış ve mIRF1 mRNA seviyesinde azalma olduğunu gözlemledi.Sonuç: Bu çalışmanın sonuçları UPR’ nin, doymuş lipit kaynaklı stres koşulları altında temel inflamasyonla ilişkili genlerin ekspresyonunu düzenlediğini doğrulamaktadır.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA AKILILAR P (2020). Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. , 607 - 612. 10.31067/0.2019.194
Chicago AKILILAR Pelin TELKOPARAN Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. (2020): 607 - 612. 10.31067/0.2019.194
MLA AKILILAR Pelin TELKOPARAN Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. , 2020, ss.607 - 612. 10.31067/0.2019.194
AMA AKILILAR P Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. . 2020; 607 - 612. 10.31067/0.2019.194
Vancouver AKILILAR P Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. . 2020; 607 - 612. 10.31067/0.2019.194
IEEE AKILILAR P "Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line." , ss.607 - 612, 2020. 10.31067/0.2019.194
ISNAD AKILILAR, Pelin TELKOPARAN. "Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line". (2020), 607-612. https://doi.org/10.31067/0.2019.194
APA AKILILAR P (2020). Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi, 11(4), 607 - 612. 10.31067/0.2019.194
Chicago AKILILAR Pelin TELKOPARAN Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 11, no.4 (2020): 607 - 612. 10.31067/0.2019.194
MLA AKILILAR Pelin TELKOPARAN Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi, vol.11, no.4, 2020, ss.607 - 612. 10.31067/0.2019.194
AMA AKILILAR P Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi. 2020; 11(4): 607 - 612. 10.31067/0.2019.194
Vancouver AKILILAR P Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi. 2020; 11(4): 607 - 612. 10.31067/0.2019.194
IEEE AKILILAR P "Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line." Acıbadem Üniversitesi Sağlık Bilimleri Dergisi, 11, ss.607 - 612, 2020. 10.31067/0.2019.194
ISNAD AKILILAR, Pelin TELKOPARAN. "Investigation of Some Inflammation Related Gene Expressions Under Lipotoxic Endoplasmic Reticulum Stress in Mouse Macrophage Cell Line". Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 11/4 (2020), 607-612. https://doi.org/10.31067/0.2019.194