Numan BAYDİLLİ
(Erciyes Üniversitesi, Tıp Fakültesi, Üroloji Bölümü, Kayseri, Türkiye)
Emre Can AKINSAL
(Erciyes Üniversitesi, Tıp Fakültesi, Üroloji Bölümü, Kayseri, Türkiye)
Züleyha DOĞANYİĞİT
(Yozgat Bozok Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Bölümü, Yozgat, Türkiye)
Oğuz EKMEKÇİOĞLU
(Erciyes Üniversitesi, Tıp Fakültesi, Üroloji Bölümü, Kayseri, Türkiye)
Sibel SİLİCİ
(Erciyes Üniversitesi, Seyrani Tarım Fakültesi, Tarımsal Biyoteknoloji Bölümü, Kayseri, Türkiye)
Yıl: 2020Cilt: 42Sayı: 2ISSN: 2149-2247 / 2149-2549Sayfa Aralığı: 132 - 138İngilizce

59 0
The Protective Role of Poplar Propolis Against Alcohol-Induced Biochemical and Histological Changes in Liver and Testes Tissues of Rats
Objective: This study evaluated the biochemical and histopathological effects of propolis oil extract on liver and testicular tissue in rats subjected to alcohol toxicity. Materials and Methods: Sixty Wistar albino rats were randomly assigned into 6 groups: Control, Ethanol (EtOH: 20% ethanol) Propolis-1 (PR1: 100 mg/kgbw) Propolis-2 (PR2: 200 mg/kgbw) EtOH+PR1, and EtOH+PR2. Rats were administered ethanol and propolis by gastric tube for 15 days. Hematological, biochemical, and histopathological (liver and testes) parameters were examined. In addition, sperm concentration and motility were determined. Results: Increased deterioration of complete blood count parameters was statistically significant in the EtOH group when compared to the control group. Propolis was preventative for some effects of EtOH. The most pronounced preventive effect of propolis on ethanol-induced damage was observed in nucleated red blood cells (NRBC) and mean corpuscular hemoglobin concentration (MCHC) parameters (p<0.01). Cholesterol and triglyceride levels were found to be highest in the EtOH group, and propolis was found to reduce these effects of ethanol (p<0.01). While the highest value for the testosterone hormone was seen in the PR2 group, propolis was found to decrease the hormone level decline caused by ethanol especially in the EtOH+PR2 group. Although it was not statistically significant, giving rats ethanol showed reduced sperm motility and epididymal sperm concentration, whereas co-administering propolis and ethanol showed an increase in these values. Conclusion: It was found that ethanol caused negative effects on the biochemical parameters studied, and histopathological assessments also showed negative effects on liver and testicular tissue. Propolis (200 mg/kgbw), on the other hand, was found to have a mitigating effect on these values.
DergiAraştırma MakalesiErişime Açık
  • 1. Baliño P, Romero-Cano R, Sánchez-Andrés JV, Valls V, Aragón CG, Muriach M. Effects of Acute Ethanol Administration on Brain Oxidative Status: The Role of Acetaldehyde. Alcohol Clin Exp Res 2019; 43(8): 1672–81.
  • 2. Polsky S, Akturk HK. Alcohol Consumption, Diabetes Risk, and Cardiovascular Disease Within Diabetes. Curr Diab Rep 2017; 17(12): 136.
  • 3. Hosseini N, Shor J, Szabo G. Alcoholic Hepatitis: A Review. Alcohol Alcohol 2019; 54(4): 408–16.
  • 4. Abarikwu SO, Duru QC, Chinonso OV, Njoku RC. Antioxidant enzymes activity, lipid peroxidation, oxidative damage in the testis and epididymis, and steroidogenesis in rats after co-exposure to atrazine and ethanol. Andrologia 2016; 48(5): 548–57.
  • 5. Edenberg HJ, McClintick JN. Alcohol Dehydrogenases, Aldehyde Dehydrogenases, and Alcohol Use Disorders: A Critical Review. Alcohol Clin Exp Res 2018; 42(12): 2281–97.
  • 6. Kocot J, Kiełczykowska M, Luchowska-Kocot D, Kurzepa J, Musik I. Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application. Oxid Med Cell Longev 2018; 2018: 7074209.
  • 7. Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits. Oxid Med Cell Longev 2017; 2017: 1259510.
  • 8. Fuentes E, Báez ME, Bravo M, Cid C, Labra F. Determination of Total Phenolic Content in Olive Oil Samples by UV–visible Spectrometry and Multivariate Calibration. Food Analytical Methods 2012; 5(6): 1311–9.
  • 9. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999; 269(2): 337–41.
  • 10. Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Gen Pharmacol 1999; 32(6): 661–7.
  • 11. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351– 8.
  • 12. Yokoi K, Uthus EO, Nielsen FH. Nickel deficiency diminishes sperm quantity and movement in rats. Biol Trace Elem Res 2003; 93(1- 3):141–54.
  • 13. Sönmez M, Türk G, Yüce A. The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology 2005; 63(7): 2063–72.
  • 14. Evans G, Maxwell WMC. Salamon’s Artificial Insemination of Sheep and Goats. London; Butterworth-Heinemann Ltd: 1987.
  • 15. Johnsen SG. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones 1970; 1(1): 2–25.
  • 16. Catchpole O, Mitchell K, Bloor S, Davis P, Suddes A. Antiproliferative activity of New Zealand propolis and phenolic compounds vs human colorectal adenocarcinoma cells. Fitoterapia 2015; 106: 167–74.
  • 17. Pujirahayu N, Ritonga H, Uslinawaty Z. Properties and flavonoids content in propolis of some extraction method of raw propolis. Int J Pharm Pharm Sci 2014; 6(6): 338–40.
  • 18. Kubiliene L, Laugaliene V, Pavilonis A, Maruska A, Majiene D, Barcauskaite K, et al. Alternative preparation of propolis extracts: comparison of their composition and biological activities. BMC Complement Altern Med 2015; 15: 156.
  • 19. Russo A, Longo R, Vanella A. Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin. Fitoterapia 2002; 73(Suppl 1): S21–9.
  • 20. Yokoyama A, Yokoyama T, Brooks PJ, Mizukami T, Matsui T, Kimura M, et al. Macrocytosis, macrocytic anemia, and genetic polymorphisms of alcohol dehydrogenase-1B and aldehyde dehydrogenase-2 in Japanese alcoholic men. Alcohol Clin Exp Res 2014; 38(5): 1237–46.
  • 21. Colina JR, Suwalsky M, Manrique-Moreno M, Petit K, Aguilar LF, Jemiola-Rzeminska M, et al. An in vitro study of the protective effect of caffeic acid on human erythrocytes. Arch Biochem Biophys 2019; 662: 75–82.
  • 22. Kolankaya D, Selmanoğlu G, Sorkun K, Salih B. Protective effects of Turkish propolis on alcohol-induced serum lipid changes and liver injury in male rats. Food Chemistry 2002; 78(2): 213–7.
  • 23. Pari L, Karthikesan K. Protective role of caffeic acid against alcoholinduced biochemical changes in rats. Fundam Clin Pharmacol 2007; 21(4): 355–61.
  • 24. Li C, Li L, Yang CF, Zhong YJ, Wu D, Shi L, et al. Hepatoprotective effects of Methyl ferulic acid on alcohol-induced liver oxidative injury in mice by inhibiting the NOX4/ROS-MAPK pathway. Biochem Biophys Res Commun 2017; 493(1): 277–85.
  • 25. Mani F, Damasceno HC, Novelli EL, Martins EA, Sforcin JM. Propolis: Effect of different concentrations, extracts and intake period on seric biochemical variables. J Ethnopharmacol 2006; 105(1-2): 95–8.
  • 26. Russo A, Troncoso N, Sanchez F, Garbarino JA, Vanella A. Propolis protects human spermatozoa from DNA damage caused by benzo[a] pyrene and exogenous reactive oxygen species. Life Sci 2006; 78(13): 1401–6.
  • 27. Potts RJ, Notarianni LJ, Jefferies TM. Seminal plasma reduces exogenous oxidative damage to human sperm, determined by the measurement of DNA strand breaks and lipid peroxidation. Mutat Res 2000; 447(2): 249–56.
  • 28. Choi MJ, Lee EJ, Park JS, Kim SN, Park EM, Kim HS. Anti-inflammatory mechanism of galangin in lipopolysaccharide-stimulated microglia: Critical role of PPAR-γ signaling pathway. Biochem Pharmacol 2017; 144: 120–31.
  • 29. Valenzuela-Barra G, Castro C, Figueroa C, Barriga A, Silva X, de Las Heras B, et al. Anti-inflammatory activity and phenolic profile of propolis from two locations in Región Metropolitana de Santiago, Chile. J Ethnopharmacol 2015; 168: 37–44.
  • 30. Mercantepe T, Unal D, Tümkaya L, Yazici ZA. Protective effects of amifostine, curcumin and caffeic acid phenethyl ester against cisplatin-induced testis tissue damage in rats. Exp Ther Med 2018; 15(4): 3404–12.

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © 2019 Tüm Hakları Saklıdır.