Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue

özbek, hanefi; yasar sirin, duygu; Akalan, Hande; Kaya, Yasin Emre; YILMAZ, İbrahim; Karaarslan, Numan

doi:10.5455/annalsmedres.2020.02.171

Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue

Yasin Emre KAYA, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Ana Bilim Dalı, Bolu, Türkiye)

Hande AKALAN, (Namık Kemal Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Tekirdağ, Türkiye)

İbrahim YILMAZ, (İstanbul Medipol Üniversitesi, Tıp Fakültesi, Tıbbi Farmakoloji Ana Bilim Dalı, İstanbul, Türkiye)

NUMAN KARAARSLAN, (Namık Kemal Üniversitesi, Tıp Fakültesi, Nöroşirurji Ana Bilim Dalı, Tekirdağ, Türkiye)

Duygu YAŞAR ŞİRİN, (Namık Kemal Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Tekirdağ, Türkiye)

Hanefi ÖZBEK (İstanbul Medipol Üniversitesi, Tıp Fakültesi, Tıbbi Farmakoloji Ana Bilim Dalı, İstanbul, Türkiye)

Annals of Medical Research

8 3

Yıl: 2020 Cilt: 27 Sayı: 3 Sayfa Aralığı: 711 - 716 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2020.02.171 İndeks Tarihi: 20-10-2020

Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue

Öz:

Aim: A major cause of low back pain is disc degeneration. Nevertheless, no specific and reliable markers of the degeneration of thenucleus pulposus (NP) are available. This presented study aimed to examine changes in the expressions of genes in primary cellcultures isolated from intact and degenerated tissues to give insights into the biopathogenesis of intervertebral disc (IVD) tissue.Material and Methods: Tissues of eight patients (n = 8; average age: 41.74 ± 9.86 years) were resected through microdiscectomy,and primary cell cultures were prepared using degenerated disc tissue. The cultured degenerated tissues served as the study group.The samples in the control group comprised the intact tissues of patients (n = 8; average age: 38.68 ± 7.91 years) resected followinga trauma. Morphology of the cell surface were evaluated using an inverted light/fluorescent microscopy at 0 and 24 h on days 10and 21. The expressions of the chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1 beta (IL-1 beta),and matrix metalloproteinase (MMP)-7 and MMP-19 genes were evaluated using the reverse transcription-quantitative polymerasechain reaction (RT-qPCR). The data obtained were statistically analyzed.Results: The four genes investigated, except COMP (P > 0.05), changed significantly in primary cell cultures isolated from degenerativeIVD tissues. This result was statistically significant (P < 0.05). The gene expressions in the samples derived from intact IVD tissueschanged markedly and these changes were associated with proliferation (P < 0.05).Conclusion: Analyzing the changes in gene expression levels associated with IVD should contribute to future studies on theprevention and treatment of such pathologies. The data obtained from the present study will shed light on cellular-based personaltargeted therapies through which genetic information can be transmitted to cells.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Kaya Y, Akalan H, YILMAZ İ, Karaarslan N, yasar sirin d, özbek h (2020). Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. , 711 - 716. 10.5455/annalsmedres.2020.02.171
Chicago	Kaya Yasin Emre,Akalan Hande,YILMAZ İbrahim,Karaarslan Numan,yasar sirin duygu,özbek hanefi Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. (2020): 711 - 716. 10.5455/annalsmedres.2020.02.171
MLA	Kaya Yasin Emre,Akalan Hande,YILMAZ İbrahim,Karaarslan Numan,yasar sirin duygu,özbek hanefi Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. , 2020, ss.711 - 716. 10.5455/annalsmedres.2020.02.171
AMA	Kaya Y,Akalan H,YILMAZ İ,Karaarslan N,yasar sirin d,özbek h Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. . 2020; 711 - 716. 10.5455/annalsmedres.2020.02.171
Vancouver	Kaya Y,Akalan H,YILMAZ İ,Karaarslan N,yasar sirin d,özbek h Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. . 2020; 711 - 716. 10.5455/annalsmedres.2020.02.171
IEEE	Kaya Y,Akalan H,YILMAZ İ,Karaarslan N,yasar sirin d,özbek h "Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue." , ss.711 - 716, 2020. 10.5455/annalsmedres.2020.02.171
ISNAD	Kaya, Yasin Emre vd. "Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue". (2020), 711-716. https://doi.org/10.5455/annalsmedres.2020.02.171

APA	Kaya Y, Akalan H, YILMAZ İ, Karaarslan N, yasar sirin d, özbek h (2020). Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. Annals of Medical Research, 27(3), 711 - 716. 10.5455/annalsmedres.2020.02.171
Chicago	Kaya Yasin Emre,Akalan Hande,YILMAZ İbrahim,Karaarslan Numan,yasar sirin duygu,özbek hanefi Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. Annals of Medical Research 27, no.3 (2020): 711 - 716. 10.5455/annalsmedres.2020.02.171
MLA	Kaya Yasin Emre,Akalan Hande,YILMAZ İbrahim,Karaarslan Numan,yasar sirin duygu,özbek hanefi Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. Annals of Medical Research, vol.27, no.3, 2020, ss.711 - 716. 10.5455/annalsmedres.2020.02.171
AMA	Kaya Y,Akalan H,YILMAZ İ,Karaarslan N,yasar sirin d,özbek h Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. Annals of Medical Research. 2020; 27(3): 711 - 716. 10.5455/annalsmedres.2020.02.171
Vancouver	Kaya Y,Akalan H,YILMAZ İ,Karaarslan N,yasar sirin d,özbek h Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue. Annals of Medical Research. 2020; 27(3): 711 - 716. 10.5455/annalsmedres.2020.02.171
IEEE	Kaya Y,Akalan H,YILMAZ İ,Karaarslan N,yasar sirin d,özbek h "Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue." Annals of Medical Research, 27, ss.711 - 716, 2020. 10.5455/annalsmedres.2020.02.171
ISNAD	Kaya, Yasin Emre vd. "Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue". Annals of Medical Research 27/3 (2020), 711-716. https://doi.org/10.5455/annalsmedres.2020.02.171