Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing

Akgül, Hasan; ARAS, Mutan; KARA, İsa; Altundag Kahraman, Sevil; Ege, Bilal; Inan, Sevinc; AYDEMİR, IŞIL; Taysı, Seyithan

doi:10.5455/annalsmedres.2020.03.193

Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing

Bilal EGE, (Adıyaman Üniversitesi, Diş Hekimliği Fakültesi, Ağız Diş Çene Cerrahisi Ana Bilim Dalı, Adıyaman, Türkiye)

Mutan Hamdi ARAS, (Girilmemişş)

Hasan AKGÜL, (Akdeniz Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Antalya, Türkiye)

Muhammed İsa KARA, (Girilmemişş)

Işıl AYDEMİR, (Niğde Ömer Halisdemir Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Ana Bilim Dalı, Niğde, Türkiye)

Sevinç İNAN, (İzmir Ekonomi Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Ana Bilim Dalı, İzmir, Türkiye)

SEYİTHAN TAYSI, (Gaziantep Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Ana Bilim Dalı, Gaziantep, Türkiye)

Sevil ALTUNDAĞ KAHRAMAN (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Ağız Diş Çene Cerrahisi Ana Bilim Dalı, Ankara, Türkiye)

Annals of Medical Research

1 0

Yıl: 2020 Cilt: 27 Sayı: 3 Sayfa Aralığı: 700 - 710 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2020.03.193 İndeks Tarihi: 20-10-2020

Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing

Öz:

Aim: In this study, we aim to investigate the effects of supplementary Grape Seed Extract (GSE) and Low-Level Laser Therapy (LLLT)on fracture healing, oxidant and anti-oxidant system in experimental mandible fractures.Materials and Methods: 48 Wistar Albino rats (adult male, n=48) were used in our study. For all the subjects, a vertical fracture linethrough molar teeth in right mandibles was created and internally fixed using a four-hole microplate and four micro screws. Firstly,these subjects were randomly divided into 4 main groups (Control, GSE, LLLT, GSE+LLLLT) of 12 animals each, and then these wereindividually separated into two sub-groups of 7th and 21st days. The number of groups (n=6) was 8 in total. GSE of 300 mg/kg/day were provided to the subjects before they were sacrificed. LLLT of 23 J/cm2 was administered to two different points alongwith the fracture line at intervals of 48 hours for 7 days in the 7th day sub-groups and for 14 days in the 21st day sub-groups. Afterthe procedure, while biochemical values such as TAS, TOS and OSI are measured; histopathologically it was examined in termsof capillary number and width, inflammatory cell, fibroblast count, collagen fibers, osteoblast count, ossification and mature boneformations.Results: For biochemical analyses, there was statistically significant difference only in TAS values on either the 7th or 21st day forthe groups. Histological analyses showed that mandibular fracture healing were significantly better in the GSE and GSE+LLLT groupscompared to the control group. The group of only LLLT had limited recovery while the combination of GSE+LLLT was the best forossification.Conclusions: It is concluded that GSE may be one of the potential methods to accelerate fracture healings in mandibular fracturescommon in oral and maxillofacial surgery clinic and thus help patients recover in shorter time, and however LLLT can have positiveeffects on the process of ossification and recovery only when combined with the extract, which may result in better outcomes inclinical use.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Ege B, ARAS M, Akgül H, KARA İ, AYDEMİR I, Inan S, Taysı S, Altundag Kahraman S (2020). Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. , 700 - 710. 10.5455/annalsmedres.2020.03.193
Chicago	Ege Bilal,ARAS Mutan,Akgül Hasan,KARA İsa,AYDEMİR IŞIL,Inan Sevinc,Taysı Seyithan,Altundag Kahraman Sevil Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. (2020): 700 - 710. 10.5455/annalsmedres.2020.03.193
MLA	Ege Bilal,ARAS Mutan,Akgül Hasan,KARA İsa,AYDEMİR IŞIL,Inan Sevinc,Taysı Seyithan,Altundag Kahraman Sevil Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. , 2020, ss.700 - 710. 10.5455/annalsmedres.2020.03.193
AMA	Ege B,ARAS M,Akgül H,KARA İ,AYDEMİR I,Inan S,Taysı S,Altundag Kahraman S Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. . 2020; 700 - 710. 10.5455/annalsmedres.2020.03.193
Vancouver	Ege B,ARAS M,Akgül H,KARA İ,AYDEMİR I,Inan S,Taysı S,Altundag Kahraman S Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. . 2020; 700 - 710. 10.5455/annalsmedres.2020.03.193
IEEE	Ege B,ARAS M,Akgül H,KARA İ,AYDEMİR I,Inan S,Taysı S,Altundag Kahraman S "Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing." , ss.700 - 710, 2020. 10.5455/annalsmedres.2020.03.193
ISNAD	Ege, Bilal vd. "Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing". (2020), 700-710. https://doi.org/10.5455/annalsmedres.2020.03.193

APA	Ege B, ARAS M, Akgül H, KARA İ, AYDEMİR I, Inan S, Taysı S, Altundag Kahraman S (2020). Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. Annals of Medical Research, 27(3), 700 - 710. 10.5455/annalsmedres.2020.03.193
Chicago	Ege Bilal,ARAS Mutan,Akgül Hasan,KARA İsa,AYDEMİR IŞIL,Inan Sevinc,Taysı Seyithan,Altundag Kahraman Sevil Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. Annals of Medical Research 27, no.3 (2020): 700 - 710. 10.5455/annalsmedres.2020.03.193
MLA	Ege Bilal,ARAS Mutan,Akgül Hasan,KARA İsa,AYDEMİR IŞIL,Inan Sevinc,Taysı Seyithan,Altundag Kahraman Sevil Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. Annals of Medical Research, vol.27, no.3, 2020, ss.700 - 710. 10.5455/annalsmedres.2020.03.193
AMA	Ege B,ARAS M,Akgül H,KARA İ,AYDEMİR I,Inan S,Taysı S,Altundag Kahraman S Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. Annals of Medical Research. 2020; 27(3): 700 - 710. 10.5455/annalsmedres.2020.03.193
Vancouver	Ege B,ARAS M,Akgül H,KARA İ,AYDEMİR I,Inan S,Taysı S,Altundag Kahraman S Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing. Annals of Medical Research. 2020; 27(3): 700 - 710. 10.5455/annalsmedres.2020.03.193
IEEE	Ege B,ARAS M,Akgül H,KARA İ,AYDEMİR I,Inan S,Taysı S,Altundag Kahraman S "Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing." Annals of Medical Research, 27, ss.700 - 710, 2020. 10.5455/annalsmedres.2020.03.193
ISNAD	Ege, Bilal vd. "Histomorphometric analysis of the effects of grape seed extract (vitis vinifera) and low-level laser therapy (LLLT) on fracture healing". Annals of Medical Research 27/3 (2020), 700-710. https://doi.org/10.5455/annalsmedres.2020.03.193