The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease

YALIM, Zafer; Tutgun Onrat, Serap; Avsar, Alaettin; ALDEMİR, Mustafa Tayfun; YALIM, Sümeyra Alan; DOĞAN, İsmet; Onrat, Ersel

doi:10.5543/tkda.2020.15686

The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease

Zafer YALIM, (Afyonkarahisar Sağlık Bilimleri Üniversitesi, Kardiyoloji Anabilim Dalı, Afyonkarahisar, Türkiye)

Serap Tutgun ONRAT, (Afyonkarahisar Sağlık Bilimleri Üniversitesi, Medikal Genetik Anabilim Dalı, Afyonkarahisar, Türkiye)

Sümeyra Alan YALIM, (Afyonkarahisar Sağlık Bilimleri Üniversitesi, Dahiliye Anabilim Dalı, Afyonkarahisar, Türkiye)

Mustafa Tayfun ALDEMİR, (Sağlık Bilimleri Üniversitesi, Bursa Yüksek İhtisas Eğitim ve Araştırma Hastanesi, Kardiyovasküler Anabilim Dalı, Bursa, Türkiye)

Alaettin AVŞAR, (Afyonkarahisar Sağlık Bilimleri Üniversitesi, Kardiyoloji Anabilim Dalı, Afyonkarahisar, Türkiye)

İsmet DOĞAN, (Afyonkarahisar Sağlık Bilimleri Üniversitesi, Biyoistatistik Anabilim Dalı, Afyonkarahisar, Türkiye)

Ersel ONRAT (Afyonkarahisar Sağlık Bilimleri Üniversitesi, Kardiyoloji Anabilim Dalı, Afyonkarahisar, Türkiye)

Türk Kardiyoloji Derneği Arşivi

2 1

Yıl: 2020 Cilt: 48 Sayı: 5 Sayfa Aralığı: 484 - 493 Metin Dili: İngilizce DOI: 10.5543/tkda.2020.15686 İndeks Tarihi: 24-10-2020

The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease

Öz:

Objective: Peripheral artery disease (PAD) is a conditioncaused by the narrowing of limb arteries due to atherosclerosis. In recent years, polymorphisms in a number of geneshave been shown to contribute to the risk of PAD development.However, whether the contribution of these inheritable factorsis independent of traditional cardiovascular risk factors remainsunclear. This study was an investigation of the effects of diabetes mellitus (DM) and genetic background, examined singlyand together, on the pathogenesis of PAD.Methods: The effects of the factor V Leiden (G1691A), factorV H1299R, prothrombin G20210A, factor XIII V34L, B-firinogen -455 G>A, PAI-1 4G/5G, HPA1, MTHFR C677T, MTHFRA1298C, ACE I/D, APO B R3500Q, and APOE polymorphismswere evaluated using a cardiovascular disease strip assay(CVD StripAssay). Two groups were created: 100 patients withPAD (50 with DM, 50 without DM) and 60 controls without PAD(30 with DM, 30 without DM).Results: There was a signifiantly greater presence of theMTHFR A1298C and PAI 4G/5G homozygous polymorphismsin the PAD patients compared with the control group (p=0.035,p=0.004, respectively). There were no signifiant associationsbetween the other genotypes and polymorphism frequencies.In the presence of DM, the PAI-1 4G/5G homozygous polymorphism was linked to the formation of PAD (p=0.021). Regression analysis indicated that the PAI-1 4G/5G gene homozygous polymorphism demonstrated a 17.1 times greater risk forDM with PAD [95% confience interval (CI): 2.113-138.660;p=0.008] and the MTHFR A1298C homozygous polymorphismdemonstrated a 316.6 times greater risk (95% CI: 10.763-9315.342; p<0.001) for the possibility of DM with PAD.Conclusion: The MTHFR A1298C and PAI 4G/5G homozygouspolymorphisms may be associated with the development of PAD.The presence of the PAI 4G/5G homozygous polymorphism withDM was a powerful predictor for the development of PAD

Anahtar Kelime:

Periferik arter hastalığı oluşumunda genetik polimorfimin ve diabetes mellitusun etkisi

Öz:

Amaç: Periferik arter hastalığı (PAD), ateroskleroz nedeniyle ekstremite arterlerinin daralmasından kaynaklanan bir durumdur. Geçtiğimiz yıllarda, birkaç gende polimorfimin PAD geliş- me riskine katkıda bulunduğu gösterilmiştir, ancak geleneksel kardiyovasküler risk faktörlerinden bağımsız olan bu kalıtımsal faktörlerin katkısı hala belirsizliğini korumaktadır. Bu çalışmada, diabetes mellitus (DM) ve genetik arkaplanın PAD patogenezinde sadece ve birlikte etkilerini araştırdık. Yöntemler: Çalışmamızda faktör V Leiden (G1691A), Faktör V H1299R, Protrombin G20210A, Faktör XIII V34L, B-Fibrinojen-455 G> A, PAI-1 4G/5G, HPA1, MTHFR C677T, MTHFR A1298C, ACE I/D, APO B R3500Q ve APOE polimorfiminin kardiyovasküler hastalık Strip panel kullanılarak etkilerini değerlendirmeyi planladık. Hastalar iki gruba ayrıldı: PAD’lı 100 hasta (50 DM+, 50 DM-), PAD olmayan 60 kontrol hasta (30 DM+, 30 DM-). Bulgular: MTHFR A1298C ve PAI 4G/5G homozigot polimorfimi, PAD hastalarında kontrol grubuna göre anlamlı derecede yüksek bulundu (sırasıyla, p=0.035, p=0.004). Diğer genotipler ve polimorfim sıklıkları arasında anlamlı bir ilişki bulunamamıştır. Ayrıca DM varlığında PAI 4G/5G homozigot polimorfimi PAD oluşumunu etkili olduğu belirlendi (p=0.021). Regresyon analizinde PAD oluşumunda DM+ hastalarda PAI-1 4G/5G gen homozigot polimorfimi 17.1 kat daha riskli, (p=0.008) Gü- ven Aralığı (GA) %95 (2.113–138.660), MTHFR A1298C homozigos polimorfimi 316.6 kat daha riskli (p<0.001) GA %95 (10.763–9315.342) olduğu görüldü. Sonuç: MTHFR A1298C ve PAI 4G/5G homozigoz polimorfimi, PAD oluşumu ile ilişkili olabilir. PAİ 4G/5G homozigot polimorfimi, DM varlığında PAD oluşumunda güçlü bir belirleyicidir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YALIM Z, Tutgun Onrat S, YALIM S, ALDEMİR M, Avsar A, DOĞAN İ, Onrat E (2020). The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. , 484 - 493. 10.5543/tkda.2020.15686
Chicago	YALIM Zafer,Tutgun Onrat Serap,YALIM Sümeyra Alan,ALDEMİR Mustafa Tayfun,Avsar Alaettin,DOĞAN İsmet,Onrat Ersel The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. (2020): 484 - 493. 10.5543/tkda.2020.15686
MLA	YALIM Zafer,Tutgun Onrat Serap,YALIM Sümeyra Alan,ALDEMİR Mustafa Tayfun,Avsar Alaettin,DOĞAN İsmet,Onrat Ersel The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. , 2020, ss.484 - 493. 10.5543/tkda.2020.15686
AMA	YALIM Z,Tutgun Onrat S,YALIM S,ALDEMİR M,Avsar A,DOĞAN İ,Onrat E The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. . 2020; 484 - 493. 10.5543/tkda.2020.15686
Vancouver	YALIM Z,Tutgun Onrat S,YALIM S,ALDEMİR M,Avsar A,DOĞAN İ,Onrat E The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. . 2020; 484 - 493. 10.5543/tkda.2020.15686
IEEE	YALIM Z,Tutgun Onrat S,YALIM S,ALDEMİR M,Avsar A,DOĞAN İ,Onrat E "The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease." , ss.484 - 493, 2020. 10.5543/tkda.2020.15686
ISNAD	YALIM, Zafer vd. "The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease". (2020), 484-493. https://doi.org/10.5543/tkda.2020.15686

APA	YALIM Z, Tutgun Onrat S, YALIM S, ALDEMİR M, Avsar A, DOĞAN İ, Onrat E (2020). The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. Türk Kardiyoloji Derneği Arşivi, 48(5), 484 - 493. 10.5543/tkda.2020.15686
Chicago	YALIM Zafer,Tutgun Onrat Serap,YALIM Sümeyra Alan,ALDEMİR Mustafa Tayfun,Avsar Alaettin,DOĞAN İsmet,Onrat Ersel The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. Türk Kardiyoloji Derneği Arşivi 48, no.5 (2020): 484 - 493. 10.5543/tkda.2020.15686
MLA	YALIM Zafer,Tutgun Onrat Serap,YALIM Sümeyra Alan,ALDEMİR Mustafa Tayfun,Avsar Alaettin,DOĞAN İsmet,Onrat Ersel The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. Türk Kardiyoloji Derneği Arşivi, vol.48, no.5, 2020, ss.484 - 493. 10.5543/tkda.2020.15686
AMA	YALIM Z,Tutgun Onrat S,YALIM S,ALDEMİR M,Avsar A,DOĞAN İ,Onrat E The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. Türk Kardiyoloji Derneği Arşivi. 2020; 48(5): 484 - 493. 10.5543/tkda.2020.15686
Vancouver	YALIM Z,Tutgun Onrat S,YALIM S,ALDEMİR M,Avsar A,DOĞAN İ,Onrat E The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease. Türk Kardiyoloji Derneği Arşivi. 2020; 48(5): 484 - 493. 10.5543/tkda.2020.15686
IEEE	YALIM Z,Tutgun Onrat S,YALIM S,ALDEMİR M,Avsar A,DOĞAN İ,Onrat E "The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease." Türk Kardiyoloji Derneği Arşivi, 48, ss.484 - 493, 2020. 10.5543/tkda.2020.15686
ISNAD	YALIM, Zafer vd. "The effects of genetic polymorphisms and diabetes mellitus on the development of peripheral artery disease". Türk Kardiyoloji Derneği Arşivi 48/5 (2020), 484-493. https://doi.org/10.5543/tkda.2020.15686