ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population

öz, tuba; Nursal, Ayse Feyda; KAYA, İsmail; demir, osman; AYDIN, HASAN EMRE; Yigit, Serbulent

doi:10.4274/haseki.galenos.2020.6006

ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population

Tuba ÖZ, (Gaziosmanpaşa Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Tokat, Türkiye)

İsmail KAYA, (Kütahya Sağlık Bilimleri Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Kütahya, Türkiye)

Ayşe Feyda NURSAL, (Hitit Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Çorum, Türkiye)

Hasan Emre AYDIN, (Kütahya Sağlık Bilimleri Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Kütahya, Türkiye)

Osman DEMİR, (Gaziosmanpaşa Üniversitesi, Tıp Fakültesi, Biyostatik Anabilim Dalı, Tokat, Türkiye)

Serbülent YİĞİT (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Veteriner Genetiği Anabilim Dalı, Samsun, Türkiye)

Haseki Tıp Bülteni

2 0

Yıl: 2020 Cilt: 58 Sayı: 4 Sayfa Aralığı: 309 - 314 Metin Dili: İngilizce DOI: 10.4274/haseki.galenos.2020.6006 İndeks Tarihi: 24-10-2020

ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population

Öz:

Aim: Intervertebral disc degeneration (IVDD) is caused byseveral genetic and environmental factors. Aggrecan is themajor component of intervertebral disk matrix proteoglycanwith multiple functional domains. The aim of this study wasto investigate the possible association between ACAN (codingaggrecan) gene variable number tandem repeat (VNTR)polymorphism and susceptibility to IVDD.Methods: Two hundred and sixty subjects participated in thisstudy. The disease group comprised 150 patients diagnosed withsymptomatic IVDD. The control group consisted of 110 healthysubjects. The ACAN gene VNTR region was analyzed using thepolymerase chain reaction (PCR) method.Results: The most common allele in the patient and the controlgroup was 27 repeat allele (49% and 34.55%, respectively).Allele 26 was more frequent in males compared to females(p=0.030). Allele 21 and 23 were more common in ones livingin rural areas (p=0.030) while allele 27 was the most frequent inones living in urban areas (p<0.001). Allele 26, allele 29 and allele30 were less frequent in the patient group than in the controlgroup (p=0.013, p=0.001 and p=0.001, respectively) while allele27 was more common in the patient group compared to thecontrol group (p=0.001).Conclusion: Our results showed that ACAN VNTR allele 27had a positive relationship with IVDD susceptibility in a Turkishpopulation.

Anahtar Kelime:

ACAN gen VNTR Polimorfizmi ve Türk Popülasyonundaki İntervertebral Disk Dejenerasyonu

Öz:

Amaç: İntervertebral disk dejenerasyonu (İVDD) çeşitli genetik ve çevresel faktörlerden kaynaklanır. Agrekan intervertebral disk matrisi proteoglikanın çoklu fonksiyonel etki alanlarına sahip ana bileşenidir. Bu çalışmanın amacı ACAN (agrekan kodlayan) gen değişken sayılı ardışık tekrar (VNTR) polimorfizmi ve İVDD yatkınlığı arasındaki olası ilişkiyi araştırmaktır. Yöntemler: ACAN VNTR polimorfizmi ve İVDD arasındaki ilişkiyi incelemek için, 260 kişi bu çalışmaya katıldı. Hasta grubu semptomatik İVDD tanısı alan 150 hastadan oluşmuştu. Kontrol grubu 110 sağlıklı kişiden oluşmuştu. ACAN geni VNTR polimorfizmi polimeraz zincir reaksiyonu (PZR) yöntemi ile analiz edildi. Bulgular: Hasta ve kontrol grubunda en sık görülen alel 27 tekrarlı aleldi (sırasıyla, %49 ve %34,55). Alel 26 erkeklerde kadınlara göre daha sıktı (p=0,030). Alel 27 en fazla şehirde yaşayanlarda bulunurken (p<0,001), alel 21 ve 23 köyde yaşayanlarda fazlaydı (p=0,030). Alel 27 hasta grubunda kontrol grubuna göre artmışken (p=0,001), alel 26, alel 29 ve alel 30 hasta grubunda kontrol grubuna göre azdı (sırasıyla, p=0,013, p=0,001, p=0,001). Sonuç: Sonuçlarımız, Türk popülasyonunda ACAN VNTR alel 27’yi taşıyan kişilerin İVDD yatkınlığı ile pozitif ilişkisi olduğunu göstermiştir

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	öz t, KAYA İ, Nursal A, AYDIN H, demir o, Yigit S (2020). ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. , 309 - 314. 10.4274/haseki.galenos.2020.6006
Chicago	öz tuba,KAYA İsmail,Nursal Ayse Feyda,AYDIN HASAN EMRE,demir osman,Yigit Serbulent ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. (2020): 309 - 314. 10.4274/haseki.galenos.2020.6006
MLA	öz tuba,KAYA İsmail,Nursal Ayse Feyda,AYDIN HASAN EMRE,demir osman,Yigit Serbulent ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. , 2020, ss.309 - 314. 10.4274/haseki.galenos.2020.6006
AMA	öz t,KAYA İ,Nursal A,AYDIN H,demir o,Yigit S ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. . 2020; 309 - 314. 10.4274/haseki.galenos.2020.6006
Vancouver	öz t,KAYA İ,Nursal A,AYDIN H,demir o,Yigit S ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. . 2020; 309 - 314. 10.4274/haseki.galenos.2020.6006
IEEE	öz t,KAYA İ,Nursal A,AYDIN H,demir o,Yigit S "ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population." , ss.309 - 314, 2020. 10.4274/haseki.galenos.2020.6006
ISNAD	öz, tuba vd. "ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population". (2020), 309-314. https://doi.org/10.4274/haseki.galenos.2020.6006

APA	öz t, KAYA İ, Nursal A, AYDIN H, demir o, Yigit S (2020). ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. Haseki Tıp Bülteni, 58(4), 309 - 314. 10.4274/haseki.galenos.2020.6006
Chicago	öz tuba,KAYA İsmail,Nursal Ayse Feyda,AYDIN HASAN EMRE,demir osman,Yigit Serbulent ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. Haseki Tıp Bülteni 58, no.4 (2020): 309 - 314. 10.4274/haseki.galenos.2020.6006
MLA	öz tuba,KAYA İsmail,Nursal Ayse Feyda,AYDIN HASAN EMRE,demir osman,Yigit Serbulent ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. Haseki Tıp Bülteni, vol.58, no.4, 2020, ss.309 - 314. 10.4274/haseki.galenos.2020.6006
AMA	öz t,KAYA İ,Nursal A,AYDIN H,demir o,Yigit S ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. Haseki Tıp Bülteni. 2020; 58(4): 309 - 314. 10.4274/haseki.galenos.2020.6006
Vancouver	öz t,KAYA İ,Nursal A,AYDIN H,demir o,Yigit S ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population. Haseki Tıp Bülteni. 2020; 58(4): 309 - 314. 10.4274/haseki.galenos.2020.6006
IEEE	öz t,KAYA İ,Nursal A,AYDIN H,demir o,Yigit S "ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population." Haseki Tıp Bülteni, 58, ss.309 - 314, 2020. 10.4274/haseki.galenos.2020.6006
ISNAD	öz, tuba vd. "ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population". Haseki Tıp Bülteni 58/4 (2020), 309-314. https://doi.org/10.4274/haseki.galenos.2020.6006