Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water

Hatice TAKCI, (Kilis 7 Aralık Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, 79000, Kilis, Türkiye)
Pemra BAKIRHAN, (Kilis 7 Aralık Üniversitesi, Fen Bilimleri Enstitüsü, 79000, Kilis, Türkiye)
Sema GENÇ, (Kilis 7 Aralık Üniversitesi, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, 79000, Kilis, Türkiye)
Melike KAPTANOĞLU (Kahramanmaraş Sütçü İmam Üniversitesi, Fen Bilimleri Enstitüsü, 46050, Kahramanmaraş, Türkiye)
Acta Aquatica Turcica
2 0
Yıl: 2020 Cilt: 16 Sayı: 2 Sayfa Aralığı: 170 - 178 Metin Dili: İngilizce DOI: 10.22392/actaquatr.622902 İndeks Tarihi: 26-10-2020

Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water

Öz:
The production extended spectrum beta lactamase (ESBL) and metallo-beta lactamase of strains grown biofilm that wereisolated from Kilis State Hospital sewage water were investigated in this study. Congo red agar, tube adherence, tissueculture plate tests and SEM analysis were performed for the detection of biofilm production. The extended-spectrum betalactamase and metallo-beta lactamase expression of biofilm producing bacteria were tested by using Kirby-Bauer discdiffusion method according to CLSI guidelines. 5 clinical isolates were revealed to be biofilm producers. From of theseisolates, Enterococcus spp. (C2) and E. coli strains showed strong biofilm producing. One of Enterococcus isolates, SEMmicrograph of C1 has viewed the weak adherence on glass surface. Only one from 5 isolates, Enterococcus spp. (C2) thestrain was determined to synthesis ESBL enzyme. None of the strains was observed to produce metallo-beta lactamase.ESBL and biofilm production provide an important pathogenic character allowing protection against antibiotic treatments toEnterococcus spp. (C2).
Anahtar Kelime:

Atık Sudan İzole Edilen Bakteri Suşlarının Biyofilm Oluşumu ve Genişletilmiş Spektrum Beta Laktamaz (ESBL) Üretimi

Öz:
Bu çalışmada Kilis Devlet Hastanesi kanalizasyon suyundan izole edilen biyofilm oluşturan suşların genişlemiş spektrumlu beta laktamaz ve metallo-beta laktamaz üretimi incelenmiştir. Biyofilm üretiminin belirlenmesi için kongo kırmızısı agar, tüp adherens, doku kültürü plak testleri ve SEM analizi uygulanmıştır. Biyofilm üreten suşların genişlemiş spektrumlu beta laktamaz ve metallo-beta laktamaz ekspresyonu CLSI yönetmeliğine göre Kirby-Bauer disk difüzyon metodu kullanılarak test edilmiştir. 5 klinik izolatın biyofilm üreticisi olduğu belirlenmiştir. Bu izolatlardan Enterococcus spp. (C2) ve E. coli suşları güçlü biyofilm üretmiştir. Enterococcus izolatlarından C1’in SEM mikrografında cam yüzeye zayıf tutunma gözlenmiştir. 5 izolattan yalnızca biri, Enterococcus spp.’nin (C2) GSBL enzimi sentezlediği ortaya konmuştur. Suşlardan hiçbirinin metallo-beta laktamaz üretmediği belirlenmiştir. GSBL ve biyofilm üretimi Enterococcus spp. (C2) suşuna antibiyotik tedavilerine karşı koruyarak önemli bir patojenik karakter sağlamaktadır.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Adamus-Białek, W., Kubiak, A., & Czerwonka, G. (2015). Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions. Acta Biochimica Polonica, 62(4), 765-771. https://doi.org/10.18388/abp.2015_1127.
  • Akyıldız, S. (2015). Investigation of biofilm formation in the food-borne Salmonella Isolates. Dissertation, Gazi University.
  • Asati, S., & Chaudhary, U. (2018). Evaluation of two methods of biofilm detection in members of family Enterobacteriaceae. Journal of Clinical and Diagnostic Research, 12(7), 20-23. https://doi.org/10.7860/JCDR/2018/34795.11827.
  • Barnhart, M.M., & Chapman, M.R. (2006). Curli biogenesis and function. Annual Review of Microbiology, 60, 131–147. https://doi.org/10.1146/annurev.micro.60.080805.142106.
  • Bogino, P.C., Oliva Mde, L., Sorroche, F.G., & Giordano, W. (2013). The role of bacterial biofilms and surface components in plant-bacterial associations. International Journal of Molecular Sciences, 14(8), 15838- 15859. https://doi.org/10.3390/ijms140815838.
  • Christensen, G.D., Simpson, W.A., Bisno, A.L., & Beachey, E.H. (1982). Adherence of slime producing strains of Staphylococcus epidermidis to smooth surfaces. Infection and Immunity, 37(1), 318-26.
  • Darwish, S.F., & Asfour, H.A.E. (2013). Investigation of biofilm forming ability in Staphylococci causing bovine mastitis using phenotypic and genotypic assays. The Scientific World Journal 13, 1-9. http://dx.doi.org/10.1155/2013/378492.
  • Dias, C., Borges, A., Oliveira, D., Martinez-Murcia, A., Saavedra, M.J., & Simões, M. (2018). Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals. Journal of Life and Environmental Sciences, 6, e4974; https://doi.org/10.7717/peerj.4974.
  • Dufour, D., Leung, V., & Lévesque, C.M. (2012). Bacterial biofilm: structure, function, and antimicrobial resistance. Endodontic Topics, 22, 2–16. https://doi.org/10.1111/j.1601-1546.2012.00277.x.
  • Dumaru, R., Baral, R., & Shrestha, L.B. (2019). Study of biofilm formation and antibiotic resistance pattern of gram‑negative Bacilli among the clinical isolates at BPKIHS, Dharan. BMC Research Notes, 12(1), 38. http://dx.doi.org/10.1186/s13104-019-4084-8.
  • Hassan, A., Usman, J., Kaleem, F., Omair, M., Khalid, A., & Iqbal, M. (2011). Evaluation of different detection methods of biofilm formation in the clinical isolates. Brazilian Journal of Infectious Diseases, 15(4), 305- 311.
  • Ivana, C., Marija, S., Jovanka, L. Bojana, K., Nevena, B., Dubravka, M., & Ljiljana, S. (2015). Biofilm Forming Ability of Salmonella enteritidis in vitro. Acta Veterinaria-Beograd, 65(3), 1-19. https://doi.org/10.1515/acve-2015-0031.
  • Jamal, M., Tasneem, U., Hussain, T., & Andleeb, S. (2015). Bacterial biofilm: Its composition, formation and role in human infections. Research & Reviews: Journal of Microbiology and Biotechnology, 4(3), 1-14.
  • Kaiser, T.D., Pereira, M.E., dos Santos, K.R., Maciel. E.L., Schuenck, P.R., & Nunes, A.P. (2013). Modification of the Congo red agar method to detect biofilm production by Staphylococcus epidermidis. Diagnostic Microbiology and Infectious Disease, 75(3), 235-239. https://doi.org/10.1016/j.diagmicrobio.2012.11.014.
  • Karaca, B. (2011). Identification of biofilm forming abilities of Salmonella strains isolated from Turkey. Dissertation, Ankara University.
  • Kırmusaoglu, S. (2017). The comparison of methods used for the detection of biofilm formation that cause antibiotic resistance of Staphylococcus epidermidis and Staphylococcus aureus. Ortadogu Medical Journal, 9 (1), 28-33. https://doi.org/10.21601/ortadogutipdergisi.299940.
  • Lima, J.L.D.C, Alves, L.R., Paz, J.N.P.D, Rabelo, M.A., Maciel, M.A.V., & Morais, M.M.C. (2017). Analysis of biofilm production by clinical isolates of Pseudomonas aeruginosa from patients with ventilatorassociated pneumonia. Revista Brasileira de Terapia Intensiva, 29(3), 310-316. https://doi.org/ 10.5935/0103-507X.20170039.
  • Marić, S., & Vraneš, J. (2007). Characteristics and significance of microbial biofilm formation. Periodicum Biologorum, 109(2), 115-121.
  • Mathur, T., Singhal, S., Khan, S., Upadhyay, D.J., Fatma, T., & Rattan, A. (2006). Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian Journal of Medical Microbiology, 24(1), 25-29.
  • Mohamed, A., Rajaa, A.M., Khalid, Z., Fouad, M., & Naima, R. (2016). Comparison of three methods for the detection of biofilm formation by clinical isolates of Staphylococcus aureus Isolated in Casablanca. International Journal of Science and Research, 5(10), 1156-1159. https://doi.org/1159. 10.21275/ART20162319.
  • Nachammai, S.M., Jayakumar, K., & Aravazhi, A.N. (2016). The effectiveness of antibiotics against a major uropathogen- Escherichia coli and its biofilm assay by phenotypic methods. International Journal of Research in Medical Sciences, 4(11), 4820-4828. http://dx.doi.org/10.18203/2320-6012.ijrms20163773.
  • Ozdemir, F., & Arslan, S. (2018). Biofilm production and antimicrobial susceptibility profiles of Bacillus spp. from meats. Sakarya University Journal of Science, 22(6), 1674-1682. https://doi.org/10.16984/saufenbilder.395016.
  • Sala, L., Morar, A., Colibar, O., & Morvay, A.A. (2012). Antibiotic resistance of gram negative bacteria isolated from meat surface biofilm. Romanian Biotechnology Letters, 17(4), 7483-7492.
  • Saxena, N., Maheshwari, D., Dadhich, D., & Singh, S. (2014). Evaluation of Congo red agar for detection of biofilm production by various clinical candida isolates. Journal of Evolution of Medical and Dental Sciences, 3(59), 13234-13238. https://doi.org/10.14260/jemds/2014/3761.
  • Sharma, G., Sharma, S., Sharma, P., Chandola, D., Dang, S., Gupta, S., & Gabrani, R. (2016). Escherichia coli biofilm: development and therapeutic strategies. Journal of Applied Microbiology, 121(2), 309-319. https://doi.org/10.1111/jam.13078.
  • Temel, A., & Erac, B. (2018). Bacterial biofilms: detection methods and role in antibiotic resistance. Turk Mikrobiyoloji Cemiyeti Dergisi, 48(1), 1-13. https://doi.org/10.5222/TMCD.2018.00.
  • Zheng, J.X., Bai, B., Lin, Z.W., Pu, Z.Y., Yao, W.M., Chen, Z., Li, D.Y., Deng, X.B., Deng, Q.W., & Yu, Z.J. (2018). Characterization of biofilm formation by Enterococcus faecalis isolates derived from urinary tract infections in China. Journal of Medical Microbiology, 67, 60-67. https://doi.org/10.1099/jmm.0.000647.
APA MERCİMEK TAKCI H, Bakırhan P, GENÇ S, KAPTANOGLU M (2020). Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. , 170 - 178. 10.22392/actaquatr.622902
Chicago MERCİMEK TAKCI HATİCE AYSUN,Bakırhan Pemra,GENÇ Sema,KAPTANOGLU Melike Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. (2020): 170 - 178. 10.22392/actaquatr.622902
MLA MERCİMEK TAKCI HATİCE AYSUN,Bakırhan Pemra,GENÇ Sema,KAPTANOGLU Melike Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. , 2020, ss.170 - 178. 10.22392/actaquatr.622902
AMA MERCİMEK TAKCI H,Bakırhan P,GENÇ S,KAPTANOGLU M Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. . 2020; 170 - 178. 10.22392/actaquatr.622902
Vancouver MERCİMEK TAKCI H,Bakırhan P,GENÇ S,KAPTANOGLU M Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. . 2020; 170 - 178. 10.22392/actaquatr.622902
IEEE MERCİMEK TAKCI H,Bakırhan P,GENÇ S,KAPTANOGLU M "Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water." , ss.170 - 178, 2020. 10.22392/actaquatr.622902
ISNAD MERCİMEK TAKCI, HATİCE AYSUN vd. "Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water". (2020), 170-178. https://doi.org/10.22392/actaquatr.622902
APA MERCİMEK TAKCI H, Bakırhan P, GENÇ S, KAPTANOGLU M (2020). Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. Acta Aquatica Turcica, 16(2), 170 - 178. 10.22392/actaquatr.622902
Chicago MERCİMEK TAKCI HATİCE AYSUN,Bakırhan Pemra,GENÇ Sema,KAPTANOGLU Melike Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. Acta Aquatica Turcica 16, no.2 (2020): 170 - 178. 10.22392/actaquatr.622902
MLA MERCİMEK TAKCI HATİCE AYSUN,Bakırhan Pemra,GENÇ Sema,KAPTANOGLU Melike Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. Acta Aquatica Turcica, vol.16, no.2, 2020, ss.170 - 178. 10.22392/actaquatr.622902
AMA MERCİMEK TAKCI H,Bakırhan P,GENÇ S,KAPTANOGLU M Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. Acta Aquatica Turcica. 2020; 16(2): 170 - 178. 10.22392/actaquatr.622902
Vancouver MERCİMEK TAKCI H,Bakırhan P,GENÇ S,KAPTANOGLU M Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. Acta Aquatica Turcica. 2020; 16(2): 170 - 178. 10.22392/actaquatr.622902
IEEE MERCİMEK TAKCI H,Bakırhan P,GENÇ S,KAPTANOGLU M "Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water." Acta Aquatica Turcica, 16, ss.170 - 178, 2020. 10.22392/actaquatr.622902
ISNAD MERCİMEK TAKCI, HATİCE AYSUN vd. "Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water". Acta Aquatica Turcica 16/2 (2020), 170-178. https://doi.org/10.22392/actaquatr.622902
Sayfa Oluşturulma Tarihi
17-04-2024 16:32

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