An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients

ERIMSAH, SEVILAY; Yazir, Yusufhan; Duran, Bulent; KUKNER, Aysel; GONCA, süheyla

doi:10.21673/anadoluklin.544067

An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients

Sevilay Erimşah, (Bolu Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Bolu, Türkiye)

Süheyla Gonca, (Kocaeli Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kocaeli, Türkiye)

Aysel Kükner, (Yakın Doğu Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, K.K.T.C)

Bülent Duran, (Sakarya Adatıp Hastanesi, Tüp Bebek Merkezi, Sakarya, Türkiye)

Yusufhan Yazır (Kocaeli Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kocaeli, Türkiye)

ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ

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Yıl: 2020 Cilt: 25 Sayı: 1 Sayfa Aralığı: 19 - 26 Metin Dili: İngilizce DOI: 10.21673/anadoluklin.544067 İndeks Tarihi: 27-10-2020

An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients

Öz:

Aim: In this study, we evaluated the DNA integrity of sperms selected by using the conventionalswim-up and density gradient centrifugation techniques and the new nanotechnology-based microfluidic chip method in order to determine the ideal sperm selection method for the intrauterineinsemination treatment of normozoospermic infertile patients (NIPs).Materials and Methods: Semen samples obtained from 20 patients were divided into four equalfractions. Control, density gradient centrifugation, swim-up, and microfluidic chip (MC) groupswere created, and the untreated (control) and treated (other) sperm samples from the four groupswere examined for DNA integrity. Acidic aniline blue staining and the TUNEL method were usedrespectively for evaluating sperm chromatin condensation defects and DNA fragmentation. Dichlorofluorescein diacetate and flow cytometry were used to determine the reactive oxygen specieslevels.Results: We measured significantly lower values of chromatin condensation defects, DNA fragmentation and reactive oxygen species for the sperms selected with the MC method, compared to thesperms selected with the conventional methods (p<0.0001).Discussion and Conclusion: We found that the new MC method was more effective in selectingsperms with high DNA integrity, compared to the conventional methods. Accordingly, the MCmethod can be an ideal sperm selection method for use in the intrauterine insemination treatmentof NIPs with high DNA fragmentation, apoptosis, and reactive oxygen species levels.

Anahtar Kelime:

Normozoospermik İnfertil Hastaların İntrauterin İnseminasyon Tedavisi İçin İdeal Bir Sperm Seçim Yöntemi

Öz:

Amaç: Bu çalışmada normozoospermik infertil hastaların (NİH) intrauterin inseminasyon tedavisi için ideal sperm seçim yöntemini belirlemek amacıyla geleneksel yüzdürme ve yoğunluk gradyanlı santrifüjleme teknikleri ve yeni nanoteknoloji bazlı mikroakışkan çip yöntemi ile seçilen spermlerin DNA bütünlüğü değerlendirilmiştir. Gereç ve Yöntemler: Yirmi hastadan toplanan semen örnekleri dört eşit parçaya bölündü. Kontrol, yoğunluk gradyanlı santrifüjleme, yüzdürme ve mikroakışkan çip (MÇ) grupları oluşturuldu ve bu dört gruba ait işlem görmemiş (kontrol) ve görmüş (diğer) sperm örnekleri DNA bütünlüğü açısından incelendi. Sperm kromatin yoğunlaşma kusurlarını ve DNA kırıklarını değerlendirmek için asidik anilin mavisi ve TUNEL boyaması kullanıldı. Reaktif oksijen türevi seviyelerini tespit etmek için akış sitometrisi ile diklorofloresan diasetat kullanıldı. Bulgular: MÇ yöntemiyle seçilen spermlerde kromatin kondensasyon defekti, DNA fragmantasyonu ve reaktif oksijen türevi değerleri, geleneksel yöntemlerle seçilen spermlere göre anlamlı şekilde düşük bulundu (p<0,0001). Tartışma ve Sonuç: Yeni MÇ yönteminin geleneksel yöntemlere göre yüksek DNA bütünlüğüne sahip sperm seçiminde daha etkili olduğu görüldü. Buna göre MÇ yöntemi yüksek DNA kırığı, apopitoz ve reaktif oksijen türevi görülen NİH’lerin intrauterin inseminasyon tedavisinde ideal bir sperm seçim yöntemi olabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ERIMSAH S, GONCA s, KUKNER A, Duran B, Yazir Y (2020). An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. , 19 - 26. 10.21673/anadoluklin.544067
Chicago	ERIMSAH SEVILAY,GONCA süheyla,KUKNER Aysel,Duran Bulent,Yazir Yusufhan An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. (2020): 19 - 26. 10.21673/anadoluklin.544067
MLA	ERIMSAH SEVILAY,GONCA süheyla,KUKNER Aysel,Duran Bulent,Yazir Yusufhan An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. , 2020, ss.19 - 26. 10.21673/anadoluklin.544067
AMA	ERIMSAH S,GONCA s,KUKNER A,Duran B,Yazir Y An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. . 2020; 19 - 26. 10.21673/anadoluklin.544067
Vancouver	ERIMSAH S,GONCA s,KUKNER A,Duran B,Yazir Y An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. . 2020; 19 - 26. 10.21673/anadoluklin.544067
IEEE	ERIMSAH S,GONCA s,KUKNER A,Duran B,Yazir Y "An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients." , ss.19 - 26, 2020. 10.21673/anadoluklin.544067
ISNAD	ERIMSAH, SEVILAY vd. "An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients". (2020), 19-26. https://doi.org/10.21673/anadoluklin.544067

APA	ERIMSAH S, GONCA s, KUKNER A, Duran B, Yazir Y (2020). An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ, 25(1), 19 - 26. 10.21673/anadoluklin.544067
Chicago	ERIMSAH SEVILAY,GONCA süheyla,KUKNER Aysel,Duran Bulent,Yazir Yusufhan An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ 25, no.1 (2020): 19 - 26. 10.21673/anadoluklin.544067
MLA	ERIMSAH SEVILAY,GONCA süheyla,KUKNER Aysel,Duran Bulent,Yazir Yusufhan An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ, vol.25, no.1, 2020, ss.19 - 26. 10.21673/anadoluklin.544067
AMA	ERIMSAH S,GONCA s,KUKNER A,Duran B,Yazir Y An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ. 2020; 25(1): 19 - 26. 10.21673/anadoluklin.544067
Vancouver	ERIMSAH S,GONCA s,KUKNER A,Duran B,Yazir Y An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ. 2020; 25(1): 19 - 26. 10.21673/anadoluklin.544067
IEEE	ERIMSAH S,GONCA s,KUKNER A,Duran B,Yazir Y "An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients." ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ, 25, ss.19 - 26, 2020. 10.21673/anadoluklin.544067
ISNAD	ERIMSAH, SEVILAY vd. "An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients". ANADOLU KLİNİĞİ TIP BİLİMLERİ DERGİSİ 25/1 (2020), 19-26. https://doi.org/10.21673/anadoluklin.544067