Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation

WU, Yanhai; ZHANG, Huili; SHI, Ning Zhong; SHAN, Jun; XU, Yazhou; LI, Xiaona; YUAN, Ye; YANG, Guangming

Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation

Huili ZHANG, (The Second People’s Hospital of Xinxiang, Departments of Pharmacy, Henan, China)

Guangming YANG, (The Second People’s Hospital of Xinxiang, Departments of Emergency, Henan, China)

Ning Zhong SHI, (The Second People’s Hospital of Xinxiang, Departments of Cardiovasology, Henan, China)

Jun SHAN, (The Second People’s Hospital of Xinxiang, Departments of General Medicine, Henan, China)

Xiaona LI, (The Second People’s Hospital of Xinxiang, Departments of Pharmacy, Henan, China)

Yanhai WU, (The Second People’s Hospital of Xinxiang, Departments of Cardiovasology, Henan, China)

Yazhou XU, (Henan Zhongping Genetic Technology Co., Ltd., Zhengzhou, Henan, China)

Ye YUAN (Henan Zhongping Genetic Technology Co., Ltd., Zhengzhou, Henan, China)

The Anatolian Journal of Cardiology

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Yıl: 2020 Cilt: 23 Sayı: 6 Sayfa Aralığı: 324 - 333 Metin Dili: İngilizce İndeks Tarihi: 17-11-2020

Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation

Öz:

Objective: We aimed to find crucial microRNAs (miRNAs) associated with the development of atrial fibrillation (AF), and then try to elucidate thepossible molecular mechanisms of miRNAs in AF.Methods: The miRNA microarray, GSE68475, which included 10 right atrial appendage samples from patients with persistent AF and 11 samplesfrom patients with normal sinus rhythm, was used for the analysis. After data preprocessing, differentially expressed miRNAs were screened using limma. Target genes of miRNAs were predicted using miRWalk2.0. We then conducted functional enrichment analyses for miRNA and targetgenes. Protein-protein interaction (PPI) network and module analyses for target genes were performed. Finally, transcription factors (TFs)-targetgenes regulatory network was predicted and constructed.Results: Seven genes, including CAMK2D, IGF2R, PPP2R2A, PAX6, POU3F2, YWHAE, and AP2A2, were targeted by TFs. Among these sevengenes, CAMK2D (targeted by miR-31-5p), IGF2R (targeted by miR-204-5p), PAX6 (targeted by miR-223-3p), POU3F2 (targeted by miR-204-5p),YWHAE (targeted by miR-31-5p), and AP2A2 (targeted by miR-204-5p) belonged to the top 10 degree genes in the PPI network. Notably, MiR204-5p, miR-31-5p, and miR-223-3p had more target genes. Besides, CAMK2D was enriched in some pathways, such as adrenergic signaling incardiomyocytes pathway and cAMP signaling pathway. YWHAE was enriched in the Hippo signaling pathway.Conclusion: miR-31-5p played a crucial role in cardiomyocytes by targeting CAMK2D and YWHAE via cAMP and Hippo signaling pathways. miR204 was involved in the progression of AF by regulating its target genes IGF2R, POU3F2, and AP2A2. On the other hand, miR-223-3p functionedin AF by targeting PAX6, which was associated with the regulation of apoptosis in AF. This study would provide a theoretical basis and potentialtherapeutic targets for the treatment of AF. (Anatol J Cardiol 2020; 23: 324-33)

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APA	ZHANG H, YANG G, SHI N, SHAN J, LI X, WU Y, XU Y, YUAN Y (2020). Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. , 324 - 333.
Chicago	ZHANG Huili,YANG Guangming,SHI Ning Zhong,SHAN Jun,LI Xiaona,WU Yanhai,XU Yazhou,YUAN Ye Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. (2020): 324 - 333.
MLA	ZHANG Huili,YANG Guangming,SHI Ning Zhong,SHAN Jun,LI Xiaona,WU Yanhai,XU Yazhou,YUAN Ye Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. , 2020, ss.324 - 333.
AMA	ZHANG H,YANG G,SHI N,SHAN J,LI X,WU Y,XU Y,YUAN Y Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. . 2020; 324 - 333.
Vancouver	ZHANG H,YANG G,SHI N,SHAN J,LI X,WU Y,XU Y,YUAN Y Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. . 2020; 324 - 333.
IEEE	ZHANG H,YANG G,SHI N,SHAN J,LI X,WU Y,XU Y,YUAN Y "Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation." , ss.324 - 333, 2020.
ISNAD	ZHANG, Huili vd. "Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation". (2020), 324-333.

APA	ZHANG H, YANG G, SHI N, SHAN J, LI X, WU Y, XU Y, YUAN Y (2020). Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. The Anatolian Journal of Cardiology, 23(6), 324 - 333.
Chicago	ZHANG Huili,YANG Guangming,SHI Ning Zhong,SHAN Jun,LI Xiaona,WU Yanhai,XU Yazhou,YUAN Ye Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. The Anatolian Journal of Cardiology 23, no.6 (2020): 324 - 333.
MLA	ZHANG Huili,YANG Guangming,SHI Ning Zhong,SHAN Jun,LI Xiaona,WU Yanhai,XU Yazhou,YUAN Ye Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. The Anatolian Journal of Cardiology, vol.23, no.6, 2020, ss.324 - 333.
AMA	ZHANG H,YANG G,SHI N,SHAN J,LI X,WU Y,XU Y,YUAN Y Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. The Anatolian Journal of Cardiology. 2020; 23(6): 324 - 333.
Vancouver	ZHANG H,YANG G,SHI N,SHAN J,LI X,WU Y,XU Y,YUAN Y Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation. The Anatolian Journal of Cardiology. 2020; 23(6): 324 - 333.
IEEE	ZHANG H,YANG G,SHI N,SHAN J,LI X,WU Y,XU Y,YUAN Y "Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation." The Anatolian Journal of Cardiology, 23, ss.324 - 333, 2020.
ISNAD	ZHANG, Huili vd. "Possible key microRNAs and corresponding molecular mechanisms for atrial fibrillation". The Anatolian Journal of Cardiology 23/6 (2020), 324-333.