Yıl: 2020 Cilt: 8 Sayı: 5 Sayfa Aralığı: 1166 - 1170 Metin Dili: İngilizce DOI: 10.24925/turjaf.v8i5.1166-1170.3337 İndeks Tarihi: 08-11-2020

The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves

Öz:
Pneumonic Pasteurellosis (PP) is an infectious disease caused by Pasteurella multocida andMannheimia haemolytica, mostly observed in cattle, sheep and calves. PP is characterized byfibrinous bronchopneumonia and pleuritis in the lungs. In this study, it was aimed to determineCaspase-3, Caspase-9, inducible nitric oxide synthase and neuronal nitric oxide synthaseexpressions by immunohistochemical methods in the lungs suffered from PP. For this purpose,twenty lung tissues were collected from calves with PP. For the Control Group, ten lungs of calveswere collected from Aksaray Slaughterhouse. After necropsies of calves were confirmed to be PPby bacteriological examinations. Then the routine histological process was performed to tissues,and stained by Hematoxylin & Eosin for histopathology, and Caspase-3, Caspase-9, inducible nitricoxide synthase and neuronal nitric oxide synthase antibody staining for immunohistochemistry. Theimmunohistochemical findings indicated that Caspase-3, Caspase-9, inducible nitric oxide synthaseand neuronal nitric oxide synthase positive reactions were seen in alveolar, bronchial andbronchiolar epithelia, and desquamated inflammatory cells in the lumens. In addition, the peripheralneural extensions were immunopositive for neuronal nitric oxide synthase and vascular endothelialcell were positive for inducible nitric oxide synthase. The findings can contribute to a betterunderstanding of expressions of molecules such as Caspase and nitric oxide synthase. These resultsshow that apoptosis and nitric oxide synthase expressions have triggered by airway epithelia andinflammatory cells in the lungs with Pneumonic Pasteurellosis in calves.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Atapattu DN, Czuprynski CJ. 2005. Mannheimia haemolytica leukotoxin induces apoptosis of bovine lymphoblastoid cells (BL-3) via a caspase-9-dependent mitochondrial pathway. Infect Immun., 73(9):5504-5513. DOI: 10.1128/IAI.73.9.5504- 5513.2005.
  • Boyce JD, Lo RYC, Wilkie I, Adler B. 2004. Pasteurella and mannheimia. (Gyles CL, Prescott JF, Songer JG, Thoenin CO) Pathogenesis of Bacterial Infections in Animals. Third edition. Place of publication: Ames, Iowa. Blackwell Publishing. pp:273-294. 978-0-8138-2939-5.
  • Caswell JL, Williams KJ. 2016. Respiratory system. (Maxie MG) Jubb, Kennedy, Palmer’s Pathology of Domestic Animals Volume 2. Sixth edition. Place of publication: St. Louis, Missouri. Elsevier Publishing. pp: 465–591. 978-0-7020-5318-4.
  • Çiftçi MK, Ortatatlı M, Erer H, Hatipoğlu F, Özdemir Ö. 2015. Veteriner sistemik patoloji. First edition. Place of publication: Konya. Selçuk Üniversitesi Basımevi. 978-975-97351-5-9.
  • Cox RA, Jacob S, Oliveras G, Murakami K, Enkhbaatar P, Traber L, Schmalstieg FC, Herndon DN, Traber DL, Hawkins HK. 2009. Pulmonary expression of nitric oxide synthase isoforms in sheep with smoke inhalation and burn injury. Exp Lung Res., 35(2):104-118. DOI: 10.1080/01902140802446832.
  • Daff S. 2010. NO synthase: structures and mechanisms. Nitric Oxide., 23(1):1-11. DOI: 10.1016/j.niox.2010.03.001.
  • Enkhbaatar P, Murakami K, Shimoda K, Mizutani A, McGuire R, Schmalstieg F, Cox R, Hawkins H, Jodoin J, Lee S, Traber L, Herndon D, Traber D. 2003. Inhibition of neuronal nitric oxide synthase by 7-nitroindazole attenuates acute lung injury in an ovine model. Am J Physiol Regul Integr Comp Physiol., 285(2): 366-372. DOI: 10.1152/ajpregu.00148.2003.
  • Fligger JM, Waldvogel AS, Pfister H, Jungi TW. 1999. Expression of inducible nitric oxide synthase in spontaneous bovine bronchopneumonia. Vet Pathol., 36(5):397-405. DOI: 10.1354/vp.36-5-397.
  • Förstermann U, Sessa WC. 2012. Nitric oxide synthases: regulation and function. Eur Heart J., 33(7): 829–837. DOI: 10.1093/eurheartj/ehr304.
  • Gaston B, Drazen JM, Loscalzo J, Stamler JS. 1994. The biology of nitrogen oxides in the airways. Am J Respir Crit Care Med., 149: 538–551. DOI: 10.1164/ajrccm.149.2.7508323.
  • Lopez A, Martinson SA. 2017. Respiratory system, mediastinum, and pleurae. (Zachary JF) Pathologic Basis of Veterinary Disease. Sixth edition. Place of publication: St. Louis, Missouri. Elsevier Publishing. pp: 471-560. 978-0-323- 35775-3.
  • McIlwain DR, Berger T, Mak TW. 2013. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol., 5(4):a008656. DOI: 10.1101/cshperspect.a008656.
  • Periasamy S, Praveena PE, Singh N. 2018. Effects of Pasteurella multocida lipopolysaccharides on bovine leukocytes. Microb Pathog., 119:225-232. DOI: 10.1016/j.micpath.2018.04.030.
  • Philchenkov A. 2004. Caspases: potential targets for regulating cell death. J Cell Mol Med., 8(4):432-444. DOI: 10.1111/j.1582-4934.2004.tb00468.x.
  • Praveena PE, Periasamy S, Kumar AA, Singh N. 2010. Cytokine profiles, apoptosis and pathology of experimental Pasteurella multocida serotype A1 infection in mice. Res Vet Sci., 89(3):332-339. DOI: 10.1016/j.rvsc.2010.04.012.
  • Reed JC. 2000. Mechanisms of apoptosis. Am J Pathol., 157(5):1415-1430. DOI: 10.1016/S0002-9440(10)64779-7.
  • Rice JA, Carrasco-Medina L, Hodgins DC, Shewen PE. 2007. Mannheimia haemolytica and bovine respiratory disease. Anim Health Res Rev., 8(2):117-128, DOI: 10.1017/S1466252307001375.
  • Rudkowski JC, Barreiro E, Harfouche R, Goldberg P, Kishta O, D'Orleans-Juste P, Labonte J, Lesur O, Hussain SN. 2004.
  • Roles of inos and nnos in sepsis-induced pulmonary apoptosis. Am J Physiol Lung Cell Mol Physiol., 286(4):793- 800. DOI: 10.1152/ajplung.00266.2003.
  • Saunders FD, Westphal M, Enkhbaatar P, Wang J, Pazdrak K, Nakano Y, Hamahata A, Jonkam CC, Lange M, Connelly RL, Kulp GA, Cox RA, Hawkins HK, Schmalstieg FC, Horvath E, Szabo C, Traber LD, Whorton E, Herndon DN, Traber DL. 2010. Molecular biological effects of selective neuronal nitric oxide synthase inhibition in ovine lung injury. Am J Physiol Lung Cell Mol Physiol., 298(3):427-436. DOI: 10.1152/ajplung.00147.2009.
  • Singh K, Ritchey JW, Confer AW. 2011. Mannheimia haemolytica: bacterial–host interactions in bovine pneumonia. Vet Pathol., 48(2): 338-348. DOI: 10.1177/0300985810377182.
  • Sun Y, Clinkenbeard KD, Clarke C, Cudd L, Highlander SK, Dabo SM. 1999. Pasteurella haemolytica leukotoxin induced apoptosis of bovine lymphocytes involves dna fragmentation. Vet Microbiol., 65(2):153-166. DOI: 10.1016/s0378- 1135(98)00286-7.
  • Thumbikat P, Dileepan T, Kannan MS, Maheswaran SK. 2005. Mechanisms underlying Mannheimia haemolytica leukotoxin-induced oncosis and apoptosis of bovine alveolar macrophages. Microb Pathog., 38(4):161-172. DOI: 10.1016/j.micpath.2005.01.002.
  • Westphal M, Enkhbaatar P, Schmalstieg FC, Kulp GA, Traber LD, Morita N, Cox RA, Hawkins HK, Westphal-Varghese BB, Rudloff HE, Maybauer DM, Maybauer MO, Burke AS, Murakami K, Saunders F, Horvath EM, Szabo C, Traber DL. 2008. Neuronal nitric oxide synthase inhibition attenuates cardiopulmonary dysfunctions after combined burn and smoke inhalation injury in sheep. Crit Care Med., 36(4):1196- 1204. DOI: 10.1097/CCM.0b013e31816a1a0c.
  • Zhou L, Zhu DY. 2009. Neuronal nitric oxide synthase: structure, subcellular localization, regulation, and clinical implications. Nitric Oxide., 20(4):223-230. DOI: 10.1016/j.niox.2009.03.001.
APA YAVUZ O, DINCEL G (2020). The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. , 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
Chicago YAVUZ Orhan,DINCEL Gungor Cagdas The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. (2020): 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
MLA YAVUZ Orhan,DINCEL Gungor Cagdas The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. , 2020, ss.1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
AMA YAVUZ O,DINCEL G The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. . 2020; 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
Vancouver YAVUZ O,DINCEL G The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. . 2020; 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
IEEE YAVUZ O,DINCEL G "The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves." , ss.1166 - 1170, 2020. 10.24925/turjaf.v8i5.1166-1170.3337
ISNAD YAVUZ, Orhan - DINCEL, Gungor Cagdas. "The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves". (2020), 1166-1170. https://doi.org/10.24925/turjaf.v8i5.1166-1170.3337
APA YAVUZ O, DINCEL G (2020). The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 8(5), 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
Chicago YAVUZ Orhan,DINCEL Gungor Cagdas The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. Türk Tarım - Gıda Bilim ve Teknoloji dergisi 8, no.5 (2020): 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
MLA YAVUZ Orhan,DINCEL Gungor Cagdas The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, vol.8, no.5, 2020, ss.1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
AMA YAVUZ O,DINCEL G The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2020; 8(5): 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
Vancouver YAVUZ O,DINCEL G The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2020; 8(5): 1166 - 1170. 10.24925/turjaf.v8i5.1166-1170.3337
IEEE YAVUZ O,DINCEL G "The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves." Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 8, ss.1166 - 1170, 2020. 10.24925/turjaf.v8i5.1166-1170.3337
ISNAD YAVUZ, Orhan - DINCEL, Gungor Cagdas. "The Effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves". Türk Tarım - Gıda Bilim ve Teknoloji dergisi 8/5 (2020), 1166-1170. https://doi.org/10.24925/turjaf.v8i5.1166-1170.3337