Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing

Genç, Ecem; Pertek, Sinem; Ayla, Sule; Okur, Mehmet Evren; Yoltas, Aysegul; BATUR, SEBNEM; Üstündağ Okur, Neslihan

doi:10.5222/MMJ.2019.29053

Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing

Mehmet Evren OKUR, (Sağlık Bilimleri Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, İstanbul, Türkiye)

Şule AYLA, (İstanbul Medipol Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Rejeneratif ve Restoratif Tıp Araştırma Merkezi, Remer, İstanbul, Türkiye)

Şebnem BATUR, (İstanbul Üniversitesi-Cerrahpaşa, Cerrahpaşa Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul, Türkiye)

Ayşegül YOLTAŞ, (Ege Üniversitesi, Fen Fakültesi, Temel ve Endüstriyel Mikrobiyoloji Anabilim Dalı, Biyoloji Bölümü, İzmir, Türkiye)

Ecem GENÇ, (İstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Farmasötik Teknoloji Anabilim Dalı, İstanbul, Türkiye)

Sinem PERTEK, (İstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Farmasötik Teknoloji Anabilim Dalı, İstanbul, Türkiye)

Neslihan Üstündağ OKUR (Sağlık Bilimleri Üniversitesi, Eczacılık Fakültesi, Farmasötik Teknoloji Anabilim Dalı, İstanbul, Türkiye)

Medeniyet Medical Journal

9 1

Yıl: 2019 Cilt: 34 Sayı: 1 Sayfa Aralığı: 67 - 75 Metin Dili: İngilizce DOI: 10.5222/MMJ.2019.29053 İndeks Tarihi: 08-11-2020

Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing

Öz:

Aim: Pycnogenol® (PYC) are used for various medicinal purposes. The aims of the present study were toevaluate wound healing activity of PYC loaded in situ gel in mice and to investigate its antibacterial activity.Method: Temperature-sensitive in situ gel containing 5% PYC was formulated by cold method using Poloxamer 188, Poloxamer 407. Blank and drug loaded in situ gel formulations were evaluated for clarity, pH,viscosity, gelation temperature, gellation capacity. The wound healing effect was tested by in vivo woundmodel. PYC in situ gel was administrated topically at a concentration of 5% for the 10 consecutive daysafter skin injury. Wound closure was measured for 10 days and at 10th day wound healing was assessed bylevels of angiogenesis, granulation tissue thickness, epidermal and dermal regeneration. Its antimicrobialproperty was evaluated by Agar well diffusion test.Results: The clarity, pH, viscosity, gellation capacity of in situ gels were found to be satisfactory.Resultsshowed that PYC in situ gel exhibited remarkable wound healing activity with the 86.91% reduction of thewound area at the day 10 on the circular excision wound model compared to control group. Moreover PYCshowed significant effect on angiogenesis, granulation tissue thickness, epidermal and dermal regeneration compared to the control group. In addition to this, PYC demonstrated antibacterial and antifungalactivities. The most sensitive strains were B. cereus (23.66 mm), C. albicans (22.66 mm), and S. aueus (23mm).Conclusion: Results indicated that PYC in situ gel enhanced wound healing effectively, and so it may bedeveloped as a an effective agent to improve wound healing in future studies to be performed.

Anahtar Kelime:

Piknogenol İçeren In Situ Jelin Yara İyileşme Özelliği İçin Değerlendirilmesi

Öz:

Amaç: Pycnogenol® (PYC) çeşitli tıbbi amaçlar için kullanılmaktadır. Bu çalışmanın amacı, farelerde PYC yüklü in situ jelin yara iyileşme aktivitesini değerlendirmek ve antibakteriyel aktivitesini araştırmaktır. Yöntem: %5 PYC içeren sıcaklığa duyarlı in situ jel, Poloksamer 188, Poloxamer 407 kullanılarak soğuk yöntemle formüle edilmiştir. Boş ve etkin madde yüklü in situ jel formülasyonları, berraklık, pH, viskozite, jelasyon sıcaklığı, jelleşme kapasitesi açısından değerlendirilmiştir. PYC in situ jelin yara iyileştirici etkisi in vivo yara modeli ile test edildi. PYC in situ jel 10 gün boyunca topikal olarak %5’lik bir konsantrasyonda uygulandı. Yara kapanması 10 gün boyunca ölçüldü ve 10. günde yara iyileşmesi anjiyogenez, granülasyon dokusu kalınlığı, epidermal ve dermal rejenerasyon seviyeleri ile değerlendirildi. Antimikrobiyal özellik Agar well difüzyon testi ile değerlendirildi. Bulgular: In situ jellerin berraklık, pH, viskozite, jelleşme kapasitesi uygun özellikte bulunmuştur. PYC in situ jelin, yara modelinde kontrol grubuna kıyasla 10. günde yara alanında %86.91 küçülme ile belirgin yara iyileşme aktivitesine sahip olduğunu görüldü. Ayrıca PYC in situ jel, kontrol grubuna göre anjiyogenez, gra- nülasyon dokusu kalınlığı, epidermal ve dermal rejenerasyon üzerinde anlamlı bir etki göstermiştir. Buna ek olarak, PYC antibakteriyel ve antifungal aktivite göstermiştir. En hassas suşlar B. cereus (23.66 mm), C. albicans (22.66 mm), S. aueus (23 mm) olarak belirlendi. Sonuç: PYC içeren in situ jelin etkili bir şekilde yara iyileşmesini arttırdığı görülmüş ve yapılacak ileri çalış- malarla yara iyileşmesinde etkili bir ajan olarak geliştirilebileceği düşünülmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

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APA	Okur M, Ayla S, BATUR S, Yoltas A, Genç E, Pertek S, Üstündağ Okur N (2019). Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. , 67 - 75. 10.5222/MMJ.2019.29053
Chicago	Okur Mehmet Evren,Ayla Sule,BATUR SEBNEM,Yoltas Aysegul,Genç Ecem,Pertek Sinem,Üstündağ Okur Neslihan Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. (2019): 67 - 75. 10.5222/MMJ.2019.29053
MLA	Okur Mehmet Evren,Ayla Sule,BATUR SEBNEM,Yoltas Aysegul,Genç Ecem,Pertek Sinem,Üstündağ Okur Neslihan Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. , 2019, ss.67 - 75. 10.5222/MMJ.2019.29053
AMA	Okur M,Ayla S,BATUR S,Yoltas A,Genç E,Pertek S,Üstündağ Okur N Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. . 2019; 67 - 75. 10.5222/MMJ.2019.29053
Vancouver	Okur M,Ayla S,BATUR S,Yoltas A,Genç E,Pertek S,Üstündağ Okur N Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. . 2019; 67 - 75. 10.5222/MMJ.2019.29053
IEEE	Okur M,Ayla S,BATUR S,Yoltas A,Genç E,Pertek S,Üstündağ Okur N "Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing." , ss.67 - 75, 2019. 10.5222/MMJ.2019.29053
ISNAD	Okur, Mehmet Evren vd. "Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing". (2019), 67-75. https://doi.org/10.5222/MMJ.2019.29053

APA	Okur M, Ayla S, BATUR S, Yoltas A, Genç E, Pertek S, Üstündağ Okur N (2019). Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. Medeniyet Medical Journal, 34(1), 67 - 75. 10.5222/MMJ.2019.29053
Chicago	Okur Mehmet Evren,Ayla Sule,BATUR SEBNEM,Yoltas Aysegul,Genç Ecem,Pertek Sinem,Üstündağ Okur Neslihan Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. Medeniyet Medical Journal 34, no.1 (2019): 67 - 75. 10.5222/MMJ.2019.29053
MLA	Okur Mehmet Evren,Ayla Sule,BATUR SEBNEM,Yoltas Aysegul,Genç Ecem,Pertek Sinem,Üstündağ Okur Neslihan Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. Medeniyet Medical Journal, vol.34, no.1, 2019, ss.67 - 75. 10.5222/MMJ.2019.29053
AMA	Okur M,Ayla S,BATUR S,Yoltas A,Genç E,Pertek S,Üstündağ Okur N Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. Medeniyet Medical Journal. 2019; 34(1): 67 - 75. 10.5222/MMJ.2019.29053
Vancouver	Okur M,Ayla S,BATUR S,Yoltas A,Genç E,Pertek S,Üstündağ Okur N Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing. Medeniyet Medical Journal. 2019; 34(1): 67 - 75. 10.5222/MMJ.2019.29053
IEEE	Okur M,Ayla S,BATUR S,Yoltas A,Genç E,Pertek S,Üstündağ Okur N "Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing." Medeniyet Medical Journal, 34, ss.67 - 75, 2019. 10.5222/MMJ.2019.29053
ISNAD	Okur, Mehmet Evren vd. "Evaluation of In Situ Gel Containing Pycnogenol for Cutaneous Wound Healing". Medeniyet Medical Journal 34/1 (2019), 67-75. https://doi.org/10.5222/MMJ.2019.29053