Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China

WEN, Quan; YANG, Xufu; FANG, Ran; LIU-FU, Weizhen; Peng, Ling; YUAN, Xiaoqing

doi:DOI: 10.9775/kvfd.2019.23587

Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China

Ling PENG, (Yingdong College of biology and agriculture, Shaoguan University, Shaoguan, CHINA)

Xiaoqing YUAN, (Yingdong College of biology and agriculture, Shaoguan University, Shaoguan, CHINA)

Ran FANG, (Yingdong College of biology and agriculture, Shaoguan University, Shaoguan, CHINA)

Weizhen LIU-FU, (Yingdong College of biology and agriculture, Shaoguan University, Shaoguan, CHINA)

Quan WEN, (Yingdong College of biology and agriculture, Shaoguan University, Shaoguan, CHINA)

Xufu YANG (Yingdong College of biology and agriculture, Shaoguan University, Shaoguan, CHINA)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2020 Cilt: 26 Sayı: 4 Sayfa Aralığı: 491 - 497 Metin Dili: İngilizce DOI: DOI: 10.9775/kvfd.2019.23587 İndeks Tarihi: 07-11-2020

Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China

Öz:

This study analyzed the characteristics of 133 Haemophilus parasuis isolates in southern China. These isolates belonged to eleven serovars (1, 2, 4-10, 13 and 15) with 12.0% of them being characterised as non-typable. A relatively high level in resistance was encountered for trimethoprim + sulfamethoxazole (89.9%), tetracycline (75.3%), amoxicillin (69.1%), streptomycin (63.6%), carbenicillin (60.2%), kanamycin (46.6%) and ampicillin (45.6%). A total of 60% of the isolates were negative for group 1 virulence-associated autotransporters (vtaA). All group 1 vtaA negative isolates fell into polyacrylamide gel electrophoresis (PAGE) type I, while all group 1 vtaA positive isolates were classified as PAGE type II. The results of Multi-locus sequence typing (MLST) indicated a high degree of variation, 45 isolates in the study were assigned into 31 sequence types with 28 of these being new (not found in the MLST database). Antimicrobial resistance was observed in every serovar, there was no statistically significant correlation between the antimicrobial resistance and the serovars. The isolates allocated to clade 2 (based on MLST target sequences) showed the molecular characteristics of highly pathogenic strains in whole-cell protein profiling, vtaA groups 1, superoxide dismutase (sodA) sequence and MLST.

Anahtar Kelime:

Güney Çin’de Haemophilus parasuis İzolatlarının Serovarları, Antimikrobiyal Duyarlılıkları ve Moleküler Özellikleri

Öz:

Bu çalışmada, Güney Çin’deki 133 H. parasuis izolatının özellikleri analiz edildi. Bu izolatlar, %12.0’ı tiplendirilemeyen olarak karakterize edilen on bir serovara (1, 2, 4-10, 13 ve 15) aitti. Trimetoprim + sülfametoksazol (%89.9), tetrasiklin (%75.3), amoksisilin (%69.1), streptomisin (%63.6), karbenisilin (%60.2), kanamisin (%46.6) ve ampisilin (%45.6) için nispeten yüksek bir direnç seviyesine rastlandı. İzolatların %60’ı, grup 1 virülansa bağlı ototransportörler (vtaA) yönünden negatifti. Tüm grup 1 vtaA negatif izolatları poliakrilamid jel elektroforezi (PAGE) tip I’e dahil edilirken, tüm grup 1 vtaA pozitif izolatları PAGE tip II olarak sınıflandırıldı. Multi-lokus sekans tiplendirmesinin (MLST) sonuçları yüksek derecede bir varyasyon gösterdi. Çalışmadaki 45 izolat 31 sekans tipine atanırken bunların 28’inin yeni olduğu belirlendi (MLST veritabanında bulunamadı). Her serovarda antimikrobiyal direnç gözlendi, antimikrobiyal direnç ile serovarlar arasında istatistiksel olarak anlamlı bir ilişki yoktu. Clade 2’ye ayrılan izolatlar (MLST hedef sekanslarına dayanarak) hücre proteini profillemesi, vtaA grupları 1, süperoksit dismutaz (sodA) dizilimi ve MLST sonuçları patojenitesi yüksek suşların moleküler özelliklerini gösterdi.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Peng L, YUAN X, FANG R, LIU-FU W, WEN Q, YANG X (2020). Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. , 491 - 497. DOI: 10.9775/kvfd.2019.23587
Chicago	Peng Ling,YUAN Xiaoqing,FANG Ran,LIU-FU Weizhen,WEN Quan,YANG Xufu Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. (2020): 491 - 497. DOI: 10.9775/kvfd.2019.23587
MLA	Peng Ling,YUAN Xiaoqing,FANG Ran,LIU-FU Weizhen,WEN Quan,YANG Xufu Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. , 2020, ss.491 - 497. DOI: 10.9775/kvfd.2019.23587
AMA	Peng L,YUAN X,FANG R,LIU-FU W,WEN Q,YANG X Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. . 2020; 491 - 497. DOI: 10.9775/kvfd.2019.23587
Vancouver	Peng L,YUAN X,FANG R,LIU-FU W,WEN Q,YANG X Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. . 2020; 491 - 497. DOI: 10.9775/kvfd.2019.23587
IEEE	Peng L,YUAN X,FANG R,LIU-FU W,WEN Q,YANG X "Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China." , ss.491 - 497, 2020. DOI: 10.9775/kvfd.2019.23587
ISNAD	Peng, Ling vd. "Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China". (2020), 491-497. https://doi.org/DOI: 10.9775/kvfd.2019.23587

APA	Peng L, YUAN X, FANG R, LIU-FU W, WEN Q, YANG X (2020). Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(4), 491 - 497. DOI: 10.9775/kvfd.2019.23587
Chicago	Peng Ling,YUAN Xiaoqing,FANG Ran,LIU-FU Weizhen,WEN Quan,YANG Xufu Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26, no.4 (2020): 491 - 497. DOI: 10.9775/kvfd.2019.23587
MLA	Peng Ling,YUAN Xiaoqing,FANG Ran,LIU-FU Weizhen,WEN Quan,YANG Xufu Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.26, no.4, 2020, ss.491 - 497. DOI: 10.9775/kvfd.2019.23587
AMA	Peng L,YUAN X,FANG R,LIU-FU W,WEN Q,YANG X Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(4): 491 - 497. DOI: 10.9775/kvfd.2019.23587
Vancouver	Peng L,YUAN X,FANG R,LIU-FU W,WEN Q,YANG X Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(4): 491 - 497. DOI: 10.9775/kvfd.2019.23587
IEEE	Peng L,YUAN X,FANG R,LIU-FU W,WEN Q,YANG X "Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26, ss.491 - 497, 2020. DOI: 10.9775/kvfd.2019.23587
ISNAD	Peng, Ling vd. "Serovars, Antimicrobial Susceptibility and Molecular Characteristics of Haemophilus parasuis Isolates in Southern China". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26/4 (2020), 491-497. https://doi.org/DOI: 10.9775/kvfd.2019.23587