Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey

In this study, we aimed to evaluate the microbiological, molecular and pathological findings of abortus cases detected in a sheep herd consist of 200 animals. Macroscopically, irregular necrotic foci were observed in the liver in aborted fetuses. Selective and non-selective media were used for the isolation of causative agent. Phenotypic and molecular tests were performed for identification. Campylobacter fetus subsp. fetus (Cff) was isolated from organs of four foetuses aborted. While the vaginal swap samples taken from sheep that did not abort, water, feed and litter samples produced negative results for Cff, 5 of the 20 faecal samples and 5 of the vaginal swaps taken from the sheep that had aborted, yielded Cff. Multilocus Sequence Typing (MLST) was used for genotyping and all isolates were detected as Sequence Type 2 (ST2). This is the first documented report of an ovine abortion caused by Cff ST2 in Turkey. It is considered that the development and use of vaccines, containing local Campylobacter species, would contribute to both prophylaxis and control of abortions caused by campylobacters including Campylobacter fetus subsp. fetus.

Anahtar Kelime:

Türkiye’de Campylobacter fetus subsp. fetus ST2 İlişkili Koyun Abortusu

Öz:

Bu çalışmada, 200 başlık bir koyun sürüsünde görülen abortus olgularının mikrobiyolojik, moleküler ve patolojik bulgularının değerlendirilmesi amaçlandı. Aborte fetuslarda makroskopik olarak karaciğerde düzensiz nekrotik odaklar gözlendi. Etken izolasyonu için seçici ve seçici olmayan besiyerleri kullanıldı. İdentifikasyonda fenotipik ve moleküler testlerden yararlanıldı. Atık dört fötüsun organlarından Campylobacter fetus subsp. fetus (Cff) izole edildi. Abort yapmayan koyunlardan alınan vajinal swap, su, yem ve altlık örnekleri Cff yönünden negatif bulunurken, abort yapan 20 koyunun dışkı örneklerinin 5’i ve vajinal swap örneklerinin 5’i Cff pozitif olarak saptandı. Genotiplendirmede Multilokus Sekans Tiplendirme (MLST)’den yararlanıldı ve tüm izolatların ST2 (Sekans Tip 2) olduğu belirlendi. Bu çalışma, Türkiye’de koyunlarda abort olgularından Campylobacter fetus subsp. fetus ST2’nin bildirildiği ilk rapordur. Yurdumuzda abort olgularından izole edilen Campylobacter türlerini içeren aşıların geliştirilmesi ve kullanımının, Campylobacter fetus subsp. fetus’un da neden olduğu abortusların hem profilaksisine hem de kontrolüne katkıda bulunacağı düşünülmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Kısa Bildiri Erişim Türü: Erişime Açık

1. Davies P: Infertility and abortion in sheep and goats. In, Noakes DE, Parkinson TJ, England GCW (Eds): Veterinary Reproduction and Obstetrics. 10th ed., 510-525, WB Saunders, Philadelphia, 2019.
2. Erdem H, Sarıbay MK: Gebelik ve tanı yöntemleri, In, Kaymaz M, Fındık M, Rişvanlı A, Köker A (Eds): Çiftlik Hayvanlarında Doğum ve Jinekoloji. 3. Baskı, 441-452, Medipress, Malatya, 2019.
3. Taşal I, Bozkurt G: Koyunlarda abort. Türkiye Klinikleri J Vet Sci Intern Med-Special Topics, 1, 11-17, 2015.
4. Rişvanlı A, Kalkan C, Doğan H, Öcal H: Koyun ve keçilere infertilite ve yavru atma. Türkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics. 2, 18-28, 2016.
5. Lastovica AJ, Allos BM: Clinical significance of Campylobacter and related species other than Campylobacter jejuni and Campylobacter coli. In, Nachamkin I, Szymanski CM, Blaser MJ (Eds): Campylobacter, 123-150, ASM Press, Washington DC, 2008.
6. Van Bergen MAP: Subspecies differentiation and typing of Campylobacter fetus. Doctorate Thesis, University of Utrecht, 2005.
7. Carson LF, Cappellano CH: Histotechnology: A Self-instructional Text. 4th ed., 98-241, ASCP Press, Chicago, 2015.
8. Aydin F, Gümüşsoy KS, Atabay HI, Iça T, Abay S: Prevalence and distribution of Arcobacter species in various sources in Turkey and molecular analysis of isolated strains by ERIC-PCR. J Appl Microbiol, 103, 27-35, 2007. DOI: 10.1111/j.1365-2672.2006.03240.x
9. Linton D, Owen RJ, Stanley J: Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals. Res Microbiol, 147, 707-718, 1996. DOI: 10.1016/S09232508(97)85118-2
10. Wang G, Clark CG, Taylor TM, Pucknell C, Barton C, Price L, Woodward DL, Rodgers FG: Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol, 40, 4744-4747, 2002. DOI: 10.1128/JCM.40.12.4744-4747.2002
11. Schulze F, Bagon A, Müller W, Hotzel H: Identification of Campylobacter fetus subspecies by phenotypic differentiation and PCR. J Clin Microbiol, 44, 2019-2024, 2006. DOI: 10.1128/JCM.02566-05
12. Lane DJ: 16S/23S rRNA sequencing. In, Stackebrandt E, Goodfellow M (Eds): Nucleic Acid Techniques in Bacterial Systematics. 115-175, John Wiley & Sons, New York, 1991.
13. van Bergen MAP, Dingle KE, Maiden MCJ, Newell DG, van der Graaf-Van Bloois L, van Putten JPM, Wagenaar JA: Clonal nature of Campylobacter fetus as defined by multilocus sequence typing. J Clin Microbiol, 43, 5888-5898, 2005. DOI: 10.1128/JCM.43.12.5888-5898.2005
14. Diker KS, Sahal M, Aydin N: Ovine abortion associated with Campylobacter coli. Vet Rec, 122:87, 1988. DOI: 10.1136/vr.122.4.87
15. Diker KS, Istanbulluoglu E: Ovine abortion associated with Campylobacter jejuni. Vet Rec, 118:307, 1986. DOI: 10.1136/vr.118.11.307
16. Gressler LT, Kirinus JK, Machado G, Libardoni F, de Vargas AC: Campylobacter fetus subspecies fetus: Abortion and stillbirths in sheep. Cienc Rural, 42, 697-700, 2012. DOI: 10.1590/S0103-84782012000400020
17. Hum S, Quinn K, Brunner J, On SL: Evaluation of a PCR assay for identification and differentiation of Campylobacter fetus subspecies. Aust Vet J, 75, 827-831, 1997. DOI: 10.1111/j.1751-0813.1997.tb15665.x
18. Wagenaar JA, van Bergen MAP, Newell DG, Grogono-Thomas R, Duim B: Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals. J Clin Microbiol, 39, 2283-2286, 2001. DOI: 10.1128/JCM.39.6.2283-2286.2001
19. Muz A, Ertaş HB, Öngör H, Gülcü HB, Özer H, Eröksüz H, Dabak M, Başbuğ O, Kalender H: Elazığ ve çevresinde koyun ve keçilerde abortus olgularının bakteriyolojik, serolojik ve patolojik olarak incelenmesi. Turk J Vet Anim Sci, 23, 177-188, 1999.
20. Gülmez Sağlam A, Akça D, Çelebi Ö, Büyük F, Çelik E, Coşkun MR, Şahin M, Otlu S: Isolation and molecular identification of Campylobacter spp. from vaginal swab sample obtained from sheep herds with abort history. Kafkas Univ Vet Fak Derg, 25 (5): 697-701, 2019. DOI: 10.9775/ kvfd.2018.21654Ovine

APA	AYDIN F, Abay M, ATASEVER A, Çakır Bayram L, Karakaya E, ABAY S, Ekebas G, Mustak H, Gümüşsoy K, van der Graaf-van Bloois L, Diker K (2020). Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. , 557 - 562. DOI: 10.9775/kvfd.2019.23769
Chicago	AYDIN Fuat,Abay Murat,ATASEVER Ayhan,Çakır Bayram Latife,Karakaya Emre,ABAY Seçil,Ekebas Gorkem,Mustak Hamit Kaan,Gümüşsoy Kadir Semih,van der Graaf-van Bloois Linda,Diker Kadir Serdar Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. (2020): 557 - 562. DOI: 10.9775/kvfd.2019.23769
MLA	AYDIN Fuat,Abay Murat,ATASEVER Ayhan,Çakır Bayram Latife,Karakaya Emre,ABAY Seçil,Ekebas Gorkem,Mustak Hamit Kaan,Gümüşsoy Kadir Semih,van der Graaf-van Bloois Linda,Diker Kadir Serdar Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. , 2020, ss.557 - 562. DOI: 10.9775/kvfd.2019.23769
AMA	AYDIN F,Abay M,ATASEVER A,Çakır Bayram L,Karakaya E,ABAY S,Ekebas G,Mustak H,Gümüşsoy K,van der Graaf-van Bloois L,Diker K Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. . 2020; 557 - 562. DOI: 10.9775/kvfd.2019.23769
Vancouver	AYDIN F,Abay M,ATASEVER A,Çakır Bayram L,Karakaya E,ABAY S,Ekebas G,Mustak H,Gümüşsoy K,van der Graaf-van Bloois L,Diker K Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. . 2020; 557 - 562. DOI: 10.9775/kvfd.2019.23769
IEEE	AYDIN F,Abay M,ATASEVER A,Çakır Bayram L,Karakaya E,ABAY S,Ekebas G,Mustak H,Gümüşsoy K,van der Graaf-van Bloois L,Diker K "Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey." , ss.557 - 562, 2020. DOI: 10.9775/kvfd.2019.23769
ISNAD	AYDIN, Fuat vd. "Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey". (2020), 557-562. https://doi.org/DOI: 10.9775/kvfd.2019.23769

APA	AYDIN F, Abay M, ATASEVER A, Çakır Bayram L, Karakaya E, ABAY S, Ekebas G, Mustak H, Gümüşsoy K, van der Graaf-van Bloois L, Diker K (2020). Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(4), 557 - 562. DOI: 10.9775/kvfd.2019.23769
Chicago	AYDIN Fuat,Abay Murat,ATASEVER Ayhan,Çakır Bayram Latife,Karakaya Emre,ABAY Seçil,Ekebas Gorkem,Mustak Hamit Kaan,Gümüşsoy Kadir Semih,van der Graaf-van Bloois Linda,Diker Kadir Serdar Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26, no.4 (2020): 557 - 562. DOI: 10.9775/kvfd.2019.23769
MLA	AYDIN Fuat,Abay Murat,ATASEVER Ayhan,Çakır Bayram Latife,Karakaya Emre,ABAY Seçil,Ekebas Gorkem,Mustak Hamit Kaan,Gümüşsoy Kadir Semih,van der Graaf-van Bloois Linda,Diker Kadir Serdar Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.26, no.4, 2020, ss.557 - 562. DOI: 10.9775/kvfd.2019.23769
AMA	AYDIN F,Abay M,ATASEVER A,Çakır Bayram L,Karakaya E,ABAY S,Ekebas G,Mustak H,Gümüşsoy K,van der Graaf-van Bloois L,Diker K Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(4): 557 - 562. DOI: 10.9775/kvfd.2019.23769
Vancouver	AYDIN F,Abay M,ATASEVER A,Çakır Bayram L,Karakaya E,ABAY S,Ekebas G,Mustak H,Gümüşsoy K,van der Graaf-van Bloois L,Diker K Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(4): 557 - 562. DOI: 10.9775/kvfd.2019.23769
IEEE	AYDIN F,Abay M,ATASEVER A,Çakır Bayram L,Karakaya E,ABAY S,Ekebas G,Mustak H,Gümüşsoy K,van der Graaf-van Bloois L,Diker K "Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26, ss.557 - 562, 2020. DOI: 10.9775/kvfd.2019.23769
ISNAD	AYDIN, Fuat vd. "Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26/4 (2020), 557-562. https://doi.org/DOI: 10.9775/kvfd.2019.23769