Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study

Karakus, Nevin; yılmaz, kübra; Yigit, Serbulent; Gumusay, Ozge; Nursal, Ayse Feyda

doi:10.4274/haseki.galenos.2020.5992

Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study

Kübra YILMAZ, (Gaziosmanpaşa Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Tokat, Türkiye)

Özge GÜMÜŞAY, (Gaziosmanpaşa Üniversitesi, Tıp Fakültesi, Tıbbi Onkoloji Anabilim Dalı, Tokat, Türkiye)

Ayşe FEYDA NURSAL, (Hitit Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Çorum, Türkiye)

Nevin KARAKUŞ, (Gaziosmanpaşa Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Tokat, Türkiye)

Serbülent YİĞİT (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Genetik Bölümü, Samsun, Türkiye)

Haseki Tıp Bülteni

3 0

Yıl: 2020 Cilt: 58 Sayı: 3 Sayfa Aralığı: 268 - 273 Metin Dili: İngilizce DOI: 10.4274/haseki.galenos.2020.5992 İndeks Tarihi: 09-11-2020

Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study

Öz:

Aim: In this study, we investigated the association between two miRNA variants and the risk of oral squamous cell carcinoma (OSCC), and explored the interaction between clinical factors in the Turkish population.Methods: In this case control study, a total of 142 subjects were genotyped by polymerase chain reaction-restriction fragment length polymorphism to analyze miR-146aG/C (rs2910164) and miR-149C/T (rs2292832) variants. Associations between OSCC risk and clinicopathological characteristics were analyzed by chi-square testResults: There was a significant difference in genotype and allele frequencies of miR-146aG/C variant between patients and control individuals. miR-146aG/C CC genotype and C allele were higher in the patient group compared to the control group (p=0.000, p=0.0001, respectively). Significant differences were also observed when the patients and the controls were compared according to CC vs GG+GC (p=0.0002) and GG vs GC+CC (p=0.002). In combined analysis, CC-CT combined genotype increased in patient group compared to controls (p=0.002), while GC-CT combined genotype increased in controls compared to patients (p=0.028),Conclusion: Our study provides evidence that miR-146aG/C variant may play an important role in susceptibility to OSCC in the Turkish population.

Anahtar Kelime:

Oral Skuamoz Hücre Kanseri Riski ve miRNA’ların Genetik Varyasyonları: Olgu-kontrol Çalışması

Öz:

Amaç: Bu çalışmada, iki miRNA varyantı ve oral skuamoz hücreli kanser (OSHK) riski arasındaki ilişkiyi inceledik ve Türk popülasyonundaki klinik faktörlerle arasındaki etkileşimi araştırdık. Yöntemler: Bu olgu-kontrol çalışmasında, miR-146aG/C (rs2910164) ve miR-149C/T (rs2292832) varyantlarını analiz etmek için toplam 142 kişi polimeraz zincir reaksiyonu- sınırlayıcı enzim parça uzunluk polimorfizmi ile genotiplendi. OSHK ve klinikopatolojik özelliklerin ilişkisi χ2 testi ile analiz edildi. Bulgular: Hastalar ve kontrol bireyleri arasında miR-146aG/C varyant genotip ve alel sıklıklarında önemli fark vardı. miR-146aG/C CC genotipi ve C aleli hasta grubunda kontrol grubuna göre daha yüksekti (p=0,000, p=0,0001, sırasıyla). Hastalar ve kontroller kıyaslandığında CC’ye göre GG+GC (p=0,0002) ve GG’ye göre GC+CC (p=0,002) önemli farklar saptandı. Kombine analizde, GC/CT kombine genotipi kontrollerde hastalara göre artmışken (p=0,0028), CC-CT kombine genotipi hasta grubunda kontrollere göre artmıştı (p=0,002). Sonuç: Çalışmamız miR-146aG/C varyantının Türk popülasyonunda OSHK’ye yatkınlıkta önemli rol oynayabileceği kanıtlarını göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	yılmaz k, Gumusay O, Nursal A, Karakus N, Yigit S (2020). Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. , 268 - 273. 10.4274/haseki.galenos.2020.5992
Chicago	yılmaz kübra,Gumusay Ozge,Nursal Ayse Feyda,Karakus Nevin,Yigit Serbulent Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. (2020): 268 - 273. 10.4274/haseki.galenos.2020.5992
MLA	yılmaz kübra,Gumusay Ozge,Nursal Ayse Feyda,Karakus Nevin,Yigit Serbulent Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. , 2020, ss.268 - 273. 10.4274/haseki.galenos.2020.5992
AMA	yılmaz k,Gumusay O,Nursal A,Karakus N,Yigit S Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. . 2020; 268 - 273. 10.4274/haseki.galenos.2020.5992
Vancouver	yılmaz k,Gumusay O,Nursal A,Karakus N,Yigit S Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. . 2020; 268 - 273. 10.4274/haseki.galenos.2020.5992
IEEE	yılmaz k,Gumusay O,Nursal A,Karakus N,Yigit S "Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study." , ss.268 - 273, 2020. 10.4274/haseki.galenos.2020.5992
ISNAD	yılmaz, kübra vd. "Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study". (2020), 268-273. https://doi.org/10.4274/haseki.galenos.2020.5992

APA	yılmaz k, Gumusay O, Nursal A, Karakus N, Yigit S (2020). Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. Haseki Tıp Bülteni, 58(3), 268 - 273. 10.4274/haseki.galenos.2020.5992
Chicago	yılmaz kübra,Gumusay Ozge,Nursal Ayse Feyda,Karakus Nevin,Yigit Serbulent Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. Haseki Tıp Bülteni 58, no.3 (2020): 268 - 273. 10.4274/haseki.galenos.2020.5992
MLA	yılmaz kübra,Gumusay Ozge,Nursal Ayse Feyda,Karakus Nevin,Yigit Serbulent Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. Haseki Tıp Bülteni, vol.58, no.3, 2020, ss.268 - 273. 10.4274/haseki.galenos.2020.5992
AMA	yılmaz k,Gumusay O,Nursal A,Karakus N,Yigit S Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. Haseki Tıp Bülteni. 2020; 58(3): 268 - 273. 10.4274/haseki.galenos.2020.5992
Vancouver	yılmaz k,Gumusay O,Nursal A,Karakus N,Yigit S Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study. Haseki Tıp Bülteni. 2020; 58(3): 268 - 273. 10.4274/haseki.galenos.2020.5992
IEEE	yılmaz k,Gumusay O,Nursal A,Karakus N,Yigit S "Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study." Haseki Tıp Bülteni, 58, ss.268 - 273, 2020. 10.4274/haseki.galenos.2020.5992
ISNAD	yılmaz, kübra vd. "Genetic Variations of miRNAs and the Risk of Oral Squamous Cell Carcinoma: A Case-control Study". Haseki Tıp Bülteni 58/3 (2020), 268-273. https://doi.org/10.4274/haseki.galenos.2020.5992