Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats

H. Cahit ÖZCAN, (Siirt Üniversitesi, Veteriner Fakültesi, Hayvan Besleme ve Besleme Bölümü, Siirt, Türkiye)
Aydın Şükrü BENGÜ, (Bingöl Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Sağlık Hizmetleri ve Teknik Anabilim Dalı, Tıp Programı Laboratuvar Teknikleri, Bingöl, Türkiye)
H.Turan AKKOYUN, (Siirt Üniversitesi, Veteriner Fakültesi, Fizyoloji Bölümü, Siirt, Türkiye)
Aykut ULUCAN, (Bingöl Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknik Anabilim Dalı, Patoloji Programı Laboratuvar Teknikleri, Bingöl, Türkiye)
Mahire BAYRAMOGLU AKKOYUN (Siirt Üniversitesi, Veteriner Fakültesi, Biyokimya Bölümü, Siirt, Türkiye)
Erciyes Üniversitesi Veteriner Fakültesi Dergisi
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Yıl: 2020 Cilt: 17 Sayı: 1 Sayfa Aralığı: 57 - 62 Metin Dili: İngilizce DOI: 10.32707/ercivet.705004 İndeks Tarihi: 10-11-2020

Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats

Öz:
In this study, the biochemical and histopathological changes in the lungs due to lower extremity ischemiareperfusioninjury and protective effect of boric acid to prevent them were investigated. The rats were divided into threegroups as Group 1 (Sham group, n=9), Group 2 (Ischemia-reperfusion group, I/R, n=9) and Group 3 (Ischemiareperfusion,(I/R) + boric acid (BA), n=9). Anaesthesia was applied to the first group of rats without any ischemiareperfusionprocess. Following a two-hour ischemia, two hours of reperfusion, with the help of left lower extremitiestourniquet, were applied to the second group. To the third group, 200 mg/kg (i.p.) boric acid application was performed10 minutes before the ischemia was initiated and two hours of ischemia and two hours of reperfusion application werecarried out. Some antioxidant enzymes in lung tissue (SOD, GSH-Px, CAT) were analyzed. In addition the lung tissuewas evaluated histopathologically. Boric acid application was found to significantly enhance the positive effect of antioxidantenzymes. Normal histological structure was better preserved, some of macrophages were non-activated, and aremarkable reduction of neutrophil infiltration was seen after treatment in boric acid given group.
Anahtar Kelime:

Sıçanlarda Alt Ekstremite İskemi Reperfüzyonu Sonucunda Gelişen Uzak Doku Akciğer Hasarının Önlenmesinde Borik Asidin Etkilerinin İncelenmesi

Öz:
Bu çalışmada, alt ekstremite iskemi reperfüzyon hasarı sebebiyle akciğerlerde meydana gelen biyokimyasal ve histopatolojik değişiklikler ve bunlara karşı borik asidin koruyucu etkisi araştırıldı. Sıçanlar Grup 1 (Kontrol/sham grubu, n=9), Grup 2 (İskemi-reperfüzyon grubu, I/R, n=9) ve Grup 3 (İskemi-reperfüzyon, (I/R) + Borik asit (BA), n=9) olmak üzere üç gruba ayrıldı. İskemi-reperfüzyon prosesi olmayan ilk sıçan grubuna sadece anestezi uygulandı. İkinci gruba iki saatlik bir iskemi sonrası, sol alt ekstremite turnike yardımıyla iki saatlik reperfüzyon uygulandı. Üçüncü gruba, iskemi başlamadan 10 dakika önce 200 mg / kg (i.p.) borik asit uygulaması yapıldı, iki saatlik iskemi ve iki saatlik reperfüzyon uygulaması yapıldı. Akciğer dokusunda bazı antioksidan enzimlerin (SOD, GSH-Px, CAT) aktiviteleri analiz edildi. Ayrıca akciğer dokusu histopatolojik olarak değerlendirildi. Borik asit uygulamasının antioksidan enzimlerin pozitif etkisini önemli ölçüde güçlendirdiği tespit edildi. Borik asit verilen grupta tedaviden sonra normal histolojik yapının daha iyi korunduğu, bazı makrofajların aktive edilmediği ve nötrofil infiltrasyonun da belirgin bir azalmanın olduğu gözlemlendi.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Aebi H. Catalase in vitro assay methods. Methods Enzymol 1984; 105:121-6.
  • Armstrong TA, Spears JW, Lloyd KE. Inflammatory response growth and thyroid hormone concentrations are affected by long-term boron supplementation in gilts. J Anim Sci 2001; 79: 1549-56.
  • Bancroft J, Gamble M. Theory and Practice of Histological Techniques. Sixth Edition. New York: Elsevier Health Sciencesş, 2008; pp.15-25.
  • Başbuğ M, Yıldar M, Yaman İ, Özkan ÖF, Akşit H, Çavdar F, Derici H. Effects of boric acid in an experimental rat model of hepatic ischemia-reperfusion injury. Acta Medica Mediterranea 2015; 31: 1067- 73.
  • Beckers PA, Gielis JF, Van Schil PE, Adriaensen D. Lung ischemia reperfusion injury: The therapeutic role of dipeptidyl peptidase 4 inhibition. Ann Transl Med 2017; 5(6): 129.
  • Blaisdell FW. The pathophysiology of skeletal muscle ıschemia and reperfusion syndrome: A review. Cardiovascular Surgery 2002; 10(6): 620-30.
  • Büyükgüzel E, Büyükgüzel K, Snela M, Erdem M, Radtke K, Ziemnicki K, Adamski Z. Effects of boric acid on antioxidant enzyme acitivity, lipid peroxidation and ultrastructure of mitgut and fat body of galeria mellonella. Cell Biology Toxicology 2013; 29:117-129.
  • Den Hengst WA, Gielis JF, Lin JY, Van Schil PE, De Windt LJ, Moens AL. Lung ischemia-reperfusion injury: A molecular and clinical view on a complex pathophysiological process. Am J Physiol Heart Circ Physiol 2010; 299(5): H1283-99.
  • Devirian TA,Volpe SL. The physiological effects of dietary boron. Crit Rev Food Sci Nutr 2003;43(2): 219-31.
  • Eren M, Uyanik F, Karabulut N. The Effects of Dietary Boron Supplementation on performance, carcass composition and serum lipids in Japanese quails. J Anim Vet Adv 2006; 5: 1105-8.
  • Fail PA, Chapin RE, Price CJ, Heindel JJ. General, reproductive, developmental, and endocrine toxicity of boronated compounds. Reproductive Toxicology 1998; 12: 1-18.
  • Ferrari RS, Andrade CF. Oxidative stress and lung ischemia-reperfusion injury. Oxid Med Cell Longev 2015; 590987.
  • Fiette L, Slaoui M. Necropsy and Sampling Procedures in Rodents, in Drug Safety Evaluation. Springer, 2011; pp. 39-67.
  • Fischer AH, Jacobson KA, Rose J, Zeller R. Hematoxylin and Eosin Staining of Tissue and Cell Sections. Cold Spring Harbor Protocols. 2008; (5): p. pdb. Prot 4986.
  • Grace PA. Ischaemia-reperfusion injury. BJS 1994; 81: 637-47.
  • Hunt CD. Boron homeostasis in the cholecalciferoldeficient Chick. P Nat Acad Sci USA 1988; 42: 60.
  • Hunt CD. Biochemical effects of physiological amounts of dietary boron. J Trace Elem Med Biol 1996; 9:185-213.
  • Kakarla P, Vadluri G, Reddy KS. Response of hepatic antioxidant system to exercise training in aging female rat. J. Exp. Zoolog. A Comp Exp Biol 2005; 303: 203-8.
  • Klausner JM, Paterson IS, Valeri R, Hechtman HB. Limb ıschemia induced increase in permeability is mediated by leucocytes and leukotriens. Ann Surg 1988; 208:755-60.
  • Klausner JM, Paterson IS, Kobzik LESTER, Valeri CR, Shepro DAVID, Hechtman HB. Leukotrienes but not complement mediate limb ischemia-induced lung ınjury. Ann Surg 1989a; 209(4): 462-70.
  • Klausner JM, Paterson IS, Kozbik L, Valeri R, Shepro D, Hechtman HB. Oxygen free radicals mediate ischemia induced lung injury. Surgery 1989b; 105:192-9.
  • Meacham SL, Taper LJ, Volpe SL. Effects of boron supplementation on bone mineral density and dietary, blood, and urinary calcium, phosphorus, magnesium, and boron in female athletes. Environ Health Perspect 1994; 102: 79-82.
  • Paglia DE, Valentina WN. Studies on the quantitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; (70): 158-69.
  • Seekamp A, Mulligan MS, Till GO, Ward PA. Requirements for neutrophil products and l-arginine in ischemia-reperfusion injury. Am J Pathol 1993; 142 (4): 1217-26.
  • Sharma AK, Fernandez LG, Awad AS, KronI L, Laubach VE. Pro inflammatory response of alveolar epithelial cells is enhanced by alveolar macrophage -produced tnf-α during pulmonary ischemiareperfusion injury. Am J Physiol Lung Cell Mol Physiol 2007; 293(1): L105-13.
  • Sun Yi, Oberley LW, Ying Lİ. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 3413:497-500.
  • Tapiero H, Townsend DM, Tew KD. The role of carotenoids in the prevention of human pathologies. Biomed. Pharmacother 2004; 58: 100-10.
  • Tekeli A, Akgün S, Civelek A, İşbir S, Ak K, Çobanoğlu A. Alt ekstremite iskemi reperfüzyonu sonucunda gelişen akciğer hasarının önlenmesinde farklı bir ajan: FK 506 (takrolimus). Turk Gogus Kalp Dama 2001; 9(4): 242-6.
  • Tranquilli WJ, Thurmon JC, Grimm KA. Lumb and Jones' Veterinary Anesthesia and Analgesia. 4th Edition. New York: John Wiley & Sons, 2013; pp. 765-85.
  • Turkez H, Geyikoglu F, Tatar A, Keles S, Ozkanc A. Effects of some boron compounds on peripheral human blood. Zeitschrift für Natur for schung 2007; 62:889-96.
  • Uysal A, Protective Effect of melatonin on lung ınjury caused by ıschemia-reperfusion of the lower extremities. Turk Gogus Kalp Dama 2006; 14(4): 308-14.
APA ÖZCAN H, bengü a, AKKOYUN H, ULUCAN A, AKKOYUN M (2020). Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. , 57 - 62. 10.32707/ercivet.705004
Chicago ÖZCAN H. Cahit,bengü aydın şükrü,AKKOYUN H.Turan,ULUCAN Aykut,AKKOYUN Mahire BAYRAMOGLU Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. (2020): 57 - 62. 10.32707/ercivet.705004
MLA ÖZCAN H. Cahit,bengü aydın şükrü,AKKOYUN H.Turan,ULUCAN Aykut,AKKOYUN Mahire BAYRAMOGLU Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. , 2020, ss.57 - 62. 10.32707/ercivet.705004
AMA ÖZCAN H,bengü a,AKKOYUN H,ULUCAN A,AKKOYUN M Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. . 2020; 57 - 62. 10.32707/ercivet.705004
Vancouver ÖZCAN H,bengü a,AKKOYUN H,ULUCAN A,AKKOYUN M Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. . 2020; 57 - 62. 10.32707/ercivet.705004
IEEE ÖZCAN H,bengü a,AKKOYUN H,ULUCAN A,AKKOYUN M "Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats." , ss.57 - 62, 2020. 10.32707/ercivet.705004
ISNAD ÖZCAN, H. Cahit vd. "Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats". (2020), 57-62. https://doi.org/10.32707/ercivet.705004
APA ÖZCAN H, bengü a, AKKOYUN H, ULUCAN A, AKKOYUN M (2020). Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 17(1), 57 - 62. 10.32707/ercivet.705004
Chicago ÖZCAN H. Cahit,bengü aydın şükrü,AKKOYUN H.Turan,ULUCAN Aykut,AKKOYUN Mahire BAYRAMOGLU Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 17, no.1 (2020): 57 - 62. 10.32707/ercivet.705004
MLA ÖZCAN H. Cahit,bengü aydın şükrü,AKKOYUN H.Turan,ULUCAN Aykut,AKKOYUN Mahire BAYRAMOGLU Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol.17, no.1, 2020, ss.57 - 62. 10.32707/ercivet.705004
AMA ÖZCAN H,bengü a,AKKOYUN H,ULUCAN A,AKKOYUN M Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2020; 17(1): 57 - 62. 10.32707/ercivet.705004
Vancouver ÖZCAN H,bengü a,AKKOYUN H,ULUCAN A,AKKOYUN M Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2020; 17(1): 57 - 62. 10.32707/ercivet.705004
IEEE ÖZCAN H,bengü a,AKKOYUN H,ULUCAN A,AKKOYUN M "Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats." Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 17, ss.57 - 62, 2020. 10.32707/ercivet.705004
ISNAD ÖZCAN, H. Cahit vd. "Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats". Erciyes Üniversitesi Veteriner Fakültesi Dergisi 17/1 (2020), 57-62. https://doi.org/10.32707/ercivet.705004
Sayfa Oluşturulma Tarihi
23-04-2024 14:57

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