The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy

GÜNAL, Mehmet Yalçın; ÇANKAYA, Şeyda; YULUĞ, Burak; COŞKUN, Ebru; Bayraktaroglu, Zubeyir; OZANSOY, Muzaffer Beyza; Ozansoy, Mehmet; Hanoğlu, Lütfü

doi:10.30565/medalanya.553846

The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy

Mehmet OZANSOY, (Türkiye Cumhuriyeti, İstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Fizyoloji Bölümü, İstanbul, Türkiye)

Ebru COŞKUN, (Türkiye Cumhuriyeti, İstanbul Medipol Üniversitesi, Rejeneratif ve Restoratif Tıp Araştırma Merkezi, İstanbul, Türkiye)

Muzaffer Beyza OZANSOY, (Türkiye Cumhuriyeti, İstanbul Medipol Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Elektronörofizyoloji Bölümü, İstanbul, Türkiye)

Şeyda ÇANKAYA, (Alanya Alaaddin Keykubat Üniversitesi, Tıp Fakültesi, Nöroloji Bölümü, Antalya, Alanya, Türkiye)

Mehmet Yalçın GÜNAL, (Alanya Alaaddin Keykubat Üniversitesi, Tıp Fakültesi, Fizyoloji Bölümü, Antalya, Alanya, Türkiye)

Zübeyir BAYRAKTAROĞLU, (Türkiye Cumhuriyeti, İstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Fizyoloji Bölümü, İstanbul, Türkiye)

Burak YULUĞ, (Alanya Alaaddin Keykubat Üniversitesi, Tıp Fakültesi, Nöroloji Bölümü, Antalya, Alanya, Türkiye)

Lütfü HANOĞLU (Türkiye Cumhuriyeti, İstanbul Medipol Üniversitesi, Tıp Fakültesi, Nöroloji Bölümü, İstanbul, Türkiye)

Acta Medica Alanya

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Yıl: 2019 Cilt: 3 Sayı: 2 Sayfa Aralığı: 135 - 140 Metin Dili: İngilizce DOI: 10.30565/medalanya.553846 İndeks Tarihi: 12-11-2020

The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy

Öz:

Aim: Neurodegeneration caused by the axonal injury is a widely seen phenomenonin spinal cord and traumatic brain injuries. Due to the disintegration of the synapticconnection neurotrophic factors could not be transported retrogradely towards thecell body and the deprivation of the trophic factors lead to the degeneration and deathof the injured neuron. Rifampicin is an antibiotic exhibiting several neuroprotectivefunctions in various neurodegenerative conditions. Here we aim to investigate theacute neuroprotective effect of rifampicin in primary cortical neuron culture in whichneurons are axotomized by laser axotomy.Methods: Neonatal male mice were used in order to isolate cortical neurons. Isolated primary cortical neurons were cultured. After 24 hours three different rifampicinconcentrations (1 µM, 10 µM and 100 µM) were applied to the neurons and after 15minutes of rifampicin addition, neurons were laser axotomized. Viability of the cellswas evaluated by propidium iodide staining after 24 hours of axotomy.Results: Laser axotomy decreases the cortical neuron viability significantly by80.45%, while rifampicin pre-treatment increases their viability in all three dosages ina statistically significant manner.Conclusion: Rifampicin has an acute neuroprotective effect on the viability of thelaser axotomized cortical neurons.

Anahtar Kelime:

Lazer aksotomi sonrası primer kortikal nöron kültüründe rifampisinin nöronal hücre sağkalımı üzerine etkisi

Öz:

Amaç: Aksonal hasarlanma sonucu oluşan nörodejenerasyon travmatik beyin hasarı ve omurilik yaralanmalarında sıklıkla görülen bir tablodur. Sinaptik disintegrasyon nedeniyle trofik faktörlerin nöron gövdesine retrograd olarak taşınamaması sonucu trofik faktör yetersizliği aksonu hasarlanmış olan nöronun ölümüne yol açar. Rifampisin antibiyotik etkilerinin yanı sıra çeşitli nörodejeneratif süreçlerde nöroprotektif etkileri de olan bir antibiyotiktir. Bu çalışmadaki amacımız lazer aksotomi ile hasarlanmış primer kortikal nöronlarda rifampisinin akut nöroprotektif etkilerinin araştırılmasıdır. Yöntemler: Yenidoğan farelerin kortikal nöronları izole edilerek kültür edildi. Yirmi dört saat sonra seçilen üç rifampisin dozu (1 µM, 10 µM ve 100 µM) primer kortikal nöronlara uygulandı. Rifampisin uygulamasından 15 dakika sonra lazer aksotomi gerçekleştirildi. Aksotomiden 24 saat sonra propidiyum iyodit boyaması ile nöronların canlılığı ölçüldü. Bulgular: Lazer aksotomi nöronal canlılığı %80,45 oranında azalmaktadır. Buna karşılık rifampisin ön uygulaması ise aksonları hasarlı nöronların canlılığını seçilen üç dozda da anlamlı ölçüde arttırmıştır. Sonuç: Lazer aksotomi ile hasarlanmış kortikal nöronlardaki canlılığı arttırması nedeniyle rifampisinin söz konusu deneysel modelde akut nöroprotektif etkisi olduğu düşünülmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Ozansoy M, COŞKUN E, OZANSOY M, ÇANKAYA Ş, GÜNAL M, Bayraktaroglu Z, YULUĞ B, Hanoğlu L (2019). The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. , 135 - 140. 10.30565/medalanya.553846
Chicago	Ozansoy Mehmet,COŞKUN Ebru,OZANSOY Muzaffer Beyza,ÇANKAYA Şeyda,GÜNAL Mehmet Yalçın,Bayraktaroglu Zubeyir,YULUĞ Burak,Hanoğlu Lütfü The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. (2019): 135 - 140. 10.30565/medalanya.553846
MLA	Ozansoy Mehmet,COŞKUN Ebru,OZANSOY Muzaffer Beyza,ÇANKAYA Şeyda,GÜNAL Mehmet Yalçın,Bayraktaroglu Zubeyir,YULUĞ Burak,Hanoğlu Lütfü The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. , 2019, ss.135 - 140. 10.30565/medalanya.553846
AMA	Ozansoy M,COŞKUN E,OZANSOY M,ÇANKAYA Ş,GÜNAL M,Bayraktaroglu Z,YULUĞ B,Hanoğlu L The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. . 2019; 135 - 140. 10.30565/medalanya.553846
Vancouver	Ozansoy M,COŞKUN E,OZANSOY M,ÇANKAYA Ş,GÜNAL M,Bayraktaroglu Z,YULUĞ B,Hanoğlu L The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. . 2019; 135 - 140. 10.30565/medalanya.553846
IEEE	Ozansoy M,COŞKUN E,OZANSOY M,ÇANKAYA Ş,GÜNAL M,Bayraktaroglu Z,YULUĞ B,Hanoğlu L "The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy." , ss.135 - 140, 2019. 10.30565/medalanya.553846
ISNAD	Ozansoy, Mehmet vd. "The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy". (2019), 135-140. https://doi.org/10.30565/medalanya.553846

APA	Ozansoy M, COŞKUN E, OZANSOY M, ÇANKAYA Ş, GÜNAL M, Bayraktaroglu Z, YULUĞ B, Hanoğlu L (2019). The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. Acta Medica Alanya, 3(2), 135 - 140. 10.30565/medalanya.553846
Chicago	Ozansoy Mehmet,COŞKUN Ebru,OZANSOY Muzaffer Beyza,ÇANKAYA Şeyda,GÜNAL Mehmet Yalçın,Bayraktaroglu Zubeyir,YULUĞ Burak,Hanoğlu Lütfü The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. Acta Medica Alanya 3, no.2 (2019): 135 - 140. 10.30565/medalanya.553846
MLA	Ozansoy Mehmet,COŞKUN Ebru,OZANSOY Muzaffer Beyza,ÇANKAYA Şeyda,GÜNAL Mehmet Yalçın,Bayraktaroglu Zubeyir,YULUĞ Burak,Hanoğlu Lütfü The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. Acta Medica Alanya, vol.3, no.2, 2019, ss.135 - 140. 10.30565/medalanya.553846
AMA	Ozansoy M,COŞKUN E,OZANSOY M,ÇANKAYA Ş,GÜNAL M,Bayraktaroglu Z,YULUĞ B,Hanoğlu L The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. Acta Medica Alanya. 2019; 3(2): 135 - 140. 10.30565/medalanya.553846
Vancouver	Ozansoy M,COŞKUN E,OZANSOY M,ÇANKAYA Ş,GÜNAL M,Bayraktaroglu Z,YULUĞ B,Hanoğlu L The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy. Acta Medica Alanya. 2019; 3(2): 135 - 140. 10.30565/medalanya.553846
IEEE	Ozansoy M,COŞKUN E,OZANSOY M,ÇANKAYA Ş,GÜNAL M,Bayraktaroglu Z,YULUĞ B,Hanoğlu L "The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy." Acta Medica Alanya, 3, ss.135 - 140, 2019. 10.30565/medalanya.553846
ISNAD	Ozansoy, Mehmet vd. "The effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy". Acta Medica Alanya 3/2 (2019), 135-140. https://doi.org/10.30565/medalanya.553846