Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate

GÜREL, Aynur; NARTOP, Pınar; AKGÜN, İsmail Hakkı; Bedir, Erdal

Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate

Pınar NARTOP, (Namık Kemal Üniversitesi, Çorlu Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümü, Çorlu, Tekirdağ, Türkiye)

Aynur GÜREL, (Ege Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, İzmir, Türkiye)

İsmail Hakkı AKGÜN, (Ege Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, İzmir, Türkiye)

Erdal BEDİR (İzmir Yüksek Teknoloji Enstitüsü, Mühendislik Fakültesi, Biyomühendislik Bölümü, İzmir, Türkiye)

Hacettepe Journal of Biology and Chemistry

1 0

Yıl: 2019 Cilt: 47 Sayı: 2 Sayfa Aralığı: 185 - 192 Metin Dili: İngilizce İndeks Tarihi: 11-11-2020

Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate

Öz:

In this study, two grams of callus regenerated from stem and leaf explants of Astragalus trojanus Stev. were cultured in Woody Plant Medium (WPM) supplemented with 1 mg/L 2,4-D for four weeks and used as inoculum in order to investigate theeffects of working volume and media composition. The highest biomass was obtained in 250 mL flask with astragaloside IV(1.66 µg/mg) and cycloastragenol (0.19 µg/mg) accumulation. Different concentrations of sucrose and casein hydrolysate (1and 2 g/L) were also tested and the effect of pH was also investigated. Biomass accumulation cannot be enhanced, however,astragaloside IV and cycloastragenol content was ascended. The highest astragaloside IV (95.23 µg/mg) and cycloastragenol(5.93 mg/mg) accumulations were obtained at pH 6.8 and 2 g/L casein hydrolysate, respectively

Anahtar Kelime:

Astragalus trojanus Stev. Kesikli Kültürleri: pH, Sükroz ve Kazein Hidrolizat Etkisi ile Sikloartan-tip Metabolit Birikimi

Öz:

Bu çalışmada, Astragalus trojanus Stev. gövde ve yaprak eksplantlarından elde edilen iki gram ağırlığında kalluslar 1 mg/L 2,4-D içeren Woody Plant Medium (WPM) besin ortamında dört hafta boyunca kültüre alınmışlar ve çalışma hacminin ve besin ortamının etkilerinin incelenmesi için başlangıç materyali (inokulum) olarak kullanılmışlardır. En fazla biyokütle astragaloside IV (1.66 µg/mg) ve cycloastragenol (0.19 g/mg) içeriğiyle 250 ml’lik erlenden elde edilmiştir. Farklı konsantrasyonlarda sükroz ve kazein hidrolizat (1 ve 2 g/L) denemeleri yapılmış ve pH’ın etkisi de incelenmiştir. Biyokütle birikiminde artış sağlanamamış ancak astragaloside IV ve cycloastragenol içerikleri artmıştır. En yüksek astragaloside IV (95.23 µg/mg) ve cycloastragenol (5.93 µg/mg) içerikleri pH 6.8’de ve 2 g/L kazein hidrolizat denemesinde elde edilmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	NARTOP P, GÜREL A, AKGÜN İ, Bedir E (2019). Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. , 185 - 192.
Chicago	NARTOP Pınar,GÜREL Aynur,AKGÜN İsmail Hakkı,Bedir Erdal Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. (2019): 185 - 192.
MLA	NARTOP Pınar,GÜREL Aynur,AKGÜN İsmail Hakkı,Bedir Erdal Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. , 2019, ss.185 - 192.
AMA	NARTOP P,GÜREL A,AKGÜN İ,Bedir E Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. . 2019; 185 - 192.
Vancouver	NARTOP P,GÜREL A,AKGÜN İ,Bedir E Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. . 2019; 185 - 192.
IEEE	NARTOP P,GÜREL A,AKGÜN İ,Bedir E "Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate." , ss.185 - 192, 2019.
ISNAD	NARTOP, Pınar vd. "Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate". (2019), 185-192.

APA	NARTOP P, GÜREL A, AKGÜN İ, Bedir E (2019). Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. Hacettepe Journal of Biology and Chemistry, 47(2), 185 - 192.
Chicago	NARTOP Pınar,GÜREL Aynur,AKGÜN İsmail Hakkı,Bedir Erdal Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. Hacettepe Journal of Biology and Chemistry 47, no.2 (2019): 185 - 192.
MLA	NARTOP Pınar,GÜREL Aynur,AKGÜN İsmail Hakkı,Bedir Erdal Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. Hacettepe Journal of Biology and Chemistry, vol.47, no.2, 2019, ss.185 - 192.
AMA	NARTOP P,GÜREL A,AKGÜN İ,Bedir E Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. Hacettepe Journal of Biology and Chemistry. 2019; 47(2): 185 - 192.
Vancouver	NARTOP P,GÜREL A,AKGÜN İ,Bedir E Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. Hacettepe Journal of Biology and Chemistry. 2019; 47(2): 185 - 192.
IEEE	NARTOP P,GÜREL A,AKGÜN İ,Bedir E "Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate." Hacettepe Journal of Biology and Chemistry, 47, ss.185 - 192, 2019.
ISNAD	NARTOP, Pınar vd. "Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate". Hacettepe Journal of Biology and Chemistry 47/2 (2019), 185-192.