First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan
Yıl: 2020 Cilt: 26 Sayı: 1 Sayfa Aralığı: 143 - 146 Metin Dili: İngilizce DOI: 10.9775/kvfd.2019.22196 İndeks Tarihi: 15-11-2020
First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan
Öz: Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) have been investigated using species specific PCR andsequencing in 170 dogs from Kyrgyzstan. Maximum likelihood estimation (MLE) of the infection rates with 95% confidence intervals (CI) wascalculated. The molecular prevalence of hemoplasma infection was 5.29% (CI 2.57-9.34). It was found that, five (2.94%, CI 1.06-6.22) sampleswere found to be infected with Mhc, one (0.59%, CI 0.03-2.57) sample with CMhp and three (1.76%, CI 0.44-4.52) samples with both species.These results demonstrate that dogs can be exposed to each haemoplasma species and provide first molecular evidence for these speciesin Kyrgyzstan
Anahtar Kelime: Kırgızistan’da Asemptomatik Barınak Köpeklerinde Mycoplasma haemocanis ve Candidatus Mycoplasma haematoparvum İçin İlk Moleküler Kanıt
Öz: Kırgızistan’dan 170 köpekde tür spesifik PZR ve sekanslama ile Mycoplasma haemocanis (Mhc) ve Candidatus Mycoplasma haematoparvum (CMhp) araştırılmıştır. Enfeksiyon oranlarının en büyük olabilirlik tahmini (MLE) %95 güven aralığında (CI) hesaplandı. Hemoplasma enfeksiyonunun moleküler prevalansı %5.29 idi (CI 2.57-9.34). Beş (%2.94, CI 1.06-6.22) numunenin Mhc ile, bir (%0.59, CI 0.03-2.57) numunenin CMhp ile ve üç (%1.76, CI 0.44-4.52) numunenin de her iki tür ile enfekte olduğu bulundu. Bu sonuçlar, Kırgızistan’daki köpeklerin hemoplasma türlerinin her biri ile maruz kalabileceğini göstermekte ve bu türler için Kırgızistan’daki ilk moleküler kanıtı sağlamaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Kısa Bildiri Erişim Türü: Erişime Açık
- 1. Messick JB: New perspectives about Hemotrophic mycoplasma (formerly, Haemobartonella and Eperythrozoon species) infections in dogs and cats. Vet Clin North Am Small Anim Pract, 33 (6): 1453-1465, 2003. DOI: 10.1016/j.cvsm.2003.08.002
- 2. Novacco M, Meli ML, Gentilini F, Marsilio F, Ceci C, Pennisi MG, Lombardo G, Lloret A, Santos L, Carrapic OT, Willi B, Wolf G, Lutz H, Hofmann-Lehmann R: Prevalence and geographical distribution of canine hemotropic mycoplasma infections in Mediterranean countries and analysis of risk factors for infection. Vet Microbiol, 142 (3-4): 276-284, 2010. DOI: 10.1016/j.vetmic.2009.09.069
- 3. Compton SM, Maggi RG, Breitschwerdt EB: Candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis infections in dogs from the United States. Comp Immunol Microbiol Infect Dis, 35 (6): 557-562, 2012. DOI: 10.1016/j.cimid.2012.06.004
- 4. Aktas M, Ozubek S: A molecular survey of hemoplasmas in domestic dogs from Turkey. Vet Microbiol, 221, 94-97, 2018. DOI: 10.1016/j. vetmic.2018.06.004
- 5. Aquino LC, Kamani J, Haruna AM, Paludo GR, Hicks CA, Helps CR, Tasker S: Analysis of risk factors and prevalence of haemoplasma infection in dogs. Vet Parasitol, 221, 111-117, 2016. DOI: 10.1016/j. vetpar.2016.03.014
- 6. de Sousa KCM, Herrera HM, Secato CT, Oliveira ADV, Santos FM, Rocha FL, Barreto WTG, Macedo GC, de Andrade Pinto PCE, Machado RZ, Costa MT, André MR: Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland. Acta Trop, 171, 172-181, 2017. DOI: 10.1016/j.actatropica.2017.03.030
- 7. Kaewmongkol G, Lukkana N, Yangtara S, Kaewmongkol S, Thengchaisri N, Sirinarumitr T, Jittapalapong S, Fenwick SG: Association of Ehrlichia canis, Hemotropic Mycoplasma spp. and Anaplasma platys and severe anemia in dogs in Thailand. Vet Microbiol, 201, 195-200, 2017. DOI: 10.1016/j.vetmic.2017.01.022
- 8. Sharifiyazdi H, Abbaszadeh Hasiri M, Amini AH: Intravascular hemolysis associated with Candidatus Mycoplasma hematoparvum in a non-splenectomized dog in the south region of Iran. Vet Res Forum, 5 (3): 243-246, 2014.
- 9. Torkan S, Aldavood SJ, Sekhavatmandi A, Moshkelani S: Detection of haemotropic Mycoplasma (Haemobartonella) using multiplex PCR and its relationship with epidemiological factors in dogs. Comp Clin Pathol, 23 (3): 669-672, 2014. DOI: 10.1007/s00580-012-1668-2
- 10. Ravagnan S, Carli E, Piseddu E, Da Rold G, Porcellato E, Zanardello C, Carminato A, Vascellari M, Capelli G: Prevalence and molecular characterization of canine and feline hemotropic mycoplasmas (hemoplasmas) in northern Italy. Parasit Vectors, 10 (1): 132, 2017. DOI: 10.1186/s13071-017-2069-9
- 11. Altay K, Aydin MF, Aytmirzakizi A, Jumakanova Z, Cunusova A, Dumanli N: Molecular survey of hepatozoonosis in natural infected dogs: First detection and molecular characterisation of Hepatozoon canis in Kyrgyzstan. Kafkas Univ Vet Fak Derg, 25 (1): 77-81, 2019. DOI: 10.9775/ kvfd.2018.20352
- 12. Messick JB: Hemotrophic mycoplasmas (hemoplasmas): A review and new insights into pathogenic potential. Vet Clin Pathol, 33 (1): 2-13, 2004. DOI: 10.1111/j.1939-165X.2004.tb00342.x
- 13. Tasker S, Binns SH, Day MJ, Gruffdd-Jones TJ, Harbour DA, Helps CR, Jensen WA, Olver CS, Lappin MR: Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in cats in the United Kingdom. Vet Rec, 152, 193-198, 2003. DOI: 10.1136/vr.152.7.193
- 14. Aydin MF, Aktas M, Dumanli N: Molecular identification of Theileria and Babesia in sheep and goats in the Black Sea Region in Turkey. Parasitol Res, 112 (8): 2817-2824, 2013. DOI: 10.1007/s00436-013-3452-x
- 15. Maggi RG, Mascarelli PE, Havenga LN, Naidoo V, Breitschwerdt EB: Co infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian. Parasit Vectors, 6:103, 2013. DOI: 10.1186/1756-3305-6-103
| APA | ALTAY K, Aydin M, Aytmirzakızı A, Jumakanova Z, Zhunushova A, DUMANLI N (2020). First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. , 143 - 146. 10.9775/kvfd.2019.22196 |
| Chicago | ALTAY KÜRSAT,Aydin Mehmet Fatih,Aytmirzakızı Ayperi,Jumakanova Zarima,Zhunushova Aidai,DUMANLI Nazir First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. (2020): 143 - 146. 10.9775/kvfd.2019.22196 |
| MLA | ALTAY KÜRSAT,Aydin Mehmet Fatih,Aytmirzakızı Ayperi,Jumakanova Zarima,Zhunushova Aidai,DUMANLI Nazir First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. , 2020, ss.143 - 146. 10.9775/kvfd.2019.22196 |
| AMA | ALTAY K,Aydin M,Aytmirzakızı A,Jumakanova Z,Zhunushova A,DUMANLI N First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. . 2020; 143 - 146. 10.9775/kvfd.2019.22196 |
| Vancouver | ALTAY K,Aydin M,Aytmirzakızı A,Jumakanova Z,Zhunushova A,DUMANLI N First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. . 2020; 143 - 146. 10.9775/kvfd.2019.22196 |
| IEEE | ALTAY K,Aydin M,Aytmirzakızı A,Jumakanova Z,Zhunushova A,DUMANLI N "First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan." , ss.143 - 146, 2020. 10.9775/kvfd.2019.22196 |
| ISNAD | ALTAY, KÜRSAT vd. "First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan". (2020), 143-146. https://doi.org/10.9775/kvfd.2019.22196 |
| APA | ALTAY K, Aydin M, Aytmirzakızı A, Jumakanova Z, Zhunushova A, DUMANLI N (2020). First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(1), 143 - 146. 10.9775/kvfd.2019.22196 |
| Chicago | ALTAY KÜRSAT,Aydin Mehmet Fatih,Aytmirzakızı Ayperi,Jumakanova Zarima,Zhunushova Aidai,DUMANLI Nazir First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26, no.1 (2020): 143 - 146. 10.9775/kvfd.2019.22196 |
| MLA | ALTAY KÜRSAT,Aydin Mehmet Fatih,Aytmirzakızı Ayperi,Jumakanova Zarima,Zhunushova Aidai,DUMANLI Nazir First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.26, no.1, 2020, ss.143 - 146. 10.9775/kvfd.2019.22196 |
| AMA | ALTAY K,Aydin M,Aytmirzakızı A,Jumakanova Z,Zhunushova A,DUMANLI N First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(1): 143 - 146. 10.9775/kvfd.2019.22196 |
| Vancouver | ALTAY K,Aydin M,Aytmirzakızı A,Jumakanova Z,Zhunushova A,DUMANLI N First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(1): 143 - 146. 10.9775/kvfd.2019.22196 |
| IEEE | ALTAY K,Aydin M,Aytmirzakızı A,Jumakanova Z,Zhunushova A,DUMANLI N "First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26, ss.143 - 146, 2020. 10.9775/kvfd.2019.22196 |
| ISNAD | ALTAY, KÜRSAT vd. "First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26/1 (2020), 143-146. https://doi.org/10.9775/kvfd.2019.22196 |
