Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains

Öztürk, İsmail Cengiz; Kaya, Selçuk; ERMERTCAN, Şafak; nemli, salih atakan; Gul Yurtsever, Sureyya; AYGÜL, Abdurrahman

doi:DOI: 10.5152/EurJTher.2019.19062

Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains

Süreyya Gül YURTSEVER, (İzmir Katip Çelebi Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İzmir, Türkiye.)

Abdurrahman AYGÜL, (Çukurova Üniversitesi Tıp Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Adana, Türkiye.)

İsmail Cengiz ÖZTÜRK, (İzmir Katip Çelebi Üniversitesi Tıp Fakültesi Farmasötik Mikrobiyoloji Anabilim Dalı, İzmir, Türkiye)

Salih Atakan NEMLİ, (İzmir Katip Çelebi Üniversitesi Tıp Fakültesi Enfeksiyon Hastalıkları Anabilim Dalı, İzmir, Türkiye)

Selçuk KAYA, (İzmir Katip Çelebi Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İzmir, Türkiye)

Şafak ERMERTCAN (Ege Üniversitesi Tıp Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, İzmir, Türkiye)

European Journal of Therapeutics

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Yıl: 2020 Cilt: 26 Sayı: 2 Sayfa Aralığı: 113 - 118 Metin Dili: İngilizce DOI: DOI: 10.5152/EurJTher.2019.19062 İndeks Tarihi: 20-11-2020

Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains

Öz:

Objective: The objective of this study was to investigate some virulence genes and SCCmec types of methicillin-resistant Staphylococcus aureus (MRSA) isolates and to determine their relationship with virulence factors. Methods: A total of 100 MRSA strains, 64 from healthcare-associated and 36 from community-associated infections, were included in the study. The presence of mecA gene was investigated by PCR. SCCmec types and efb, clfB, agrA gene were detected by multiplex PCR and their relationship with virulence factors has been analyzed. Results: All of the isolates contain the mecA gene. At the same time, in 66 strains (66%) agrA gene, in 58 strains (58%) clfB gene, and in 47 strains (47%) efb gene were positive. In terms of SCCmec types, the distribution of these types among the 64 HA-SA strains was 53% similar-to-type-III, 16% type IV, 2% type I and 30% unclassified. The distribution of the types among the 36 CA-SA strains was 19% similar-to-type-III, 25% type IV, 8% type I and 47% unclassified, respectively. When SCCmec types were evaluated according to clinical sample type, similar-to-type-III isolates were found to be dominant in wound samples. Efb (78%), clfB (85%), agrA (88%) were the dominant genes in similar-to-type-III strains, whereas clfB (74%), agrA (100%) were the main genes detected in the type IV strains. Conclusions: It is of clinical and epidemiological importance to know the origin of MRSA strains because this affects the empirical treatment choice.

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Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Gul Yurtsever S, AYGÜL A, Öztürk İ, nemli s, Kaya S, ERMERTCAN Ş (2020). Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. , 113 - 118. DOI: 10.5152/EurJTher.2019.19062
Chicago	Gul Yurtsever Sureyya,AYGÜL Abdurrahman,Öztürk İsmail Cengiz,nemli salih atakan,Kaya Selçuk,ERMERTCAN Şafak Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. (2020): 113 - 118. DOI: 10.5152/EurJTher.2019.19062
MLA	Gul Yurtsever Sureyya,AYGÜL Abdurrahman,Öztürk İsmail Cengiz,nemli salih atakan,Kaya Selçuk,ERMERTCAN Şafak Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. , 2020, ss.113 - 118. DOI: 10.5152/EurJTher.2019.19062
AMA	Gul Yurtsever S,AYGÜL A,Öztürk İ,nemli s,Kaya S,ERMERTCAN Ş Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. . 2020; 113 - 118. DOI: 10.5152/EurJTher.2019.19062
Vancouver	Gul Yurtsever S,AYGÜL A,Öztürk İ,nemli s,Kaya S,ERMERTCAN Ş Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. . 2020; 113 - 118. DOI: 10.5152/EurJTher.2019.19062
IEEE	Gul Yurtsever S,AYGÜL A,Öztürk İ,nemli s,Kaya S,ERMERTCAN Ş "Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains." , ss.113 - 118, 2020. DOI: 10.5152/EurJTher.2019.19062
ISNAD	Gul Yurtsever, Sureyya vd. "Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains". (2020), 113-118. https://doi.org/DOI: 10.5152/EurJTher.2019.19062

APA	Gul Yurtsever S, AYGÜL A, Öztürk İ, nemli s, Kaya S, ERMERTCAN Ş (2020). Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. European Journal of Therapeutics, 26(2), 113 - 118. DOI: 10.5152/EurJTher.2019.19062
Chicago	Gul Yurtsever Sureyya,AYGÜL Abdurrahman,Öztürk İsmail Cengiz,nemli salih atakan,Kaya Selçuk,ERMERTCAN Şafak Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. European Journal of Therapeutics 26, no.2 (2020): 113 - 118. DOI: 10.5152/EurJTher.2019.19062
MLA	Gul Yurtsever Sureyya,AYGÜL Abdurrahman,Öztürk İsmail Cengiz,nemli salih atakan,Kaya Selçuk,ERMERTCAN Şafak Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. European Journal of Therapeutics, vol.26, no.2, 2020, ss.113 - 118. DOI: 10.5152/EurJTher.2019.19062
AMA	Gul Yurtsever S,AYGÜL A,Öztürk İ,nemli s,Kaya S,ERMERTCAN Ş Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. European Journal of Therapeutics. 2020; 26(2): 113 - 118. DOI: 10.5152/EurJTher.2019.19062
Vancouver	Gul Yurtsever S,AYGÜL A,Öztürk İ,nemli s,Kaya S,ERMERTCAN Ş Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains. European Journal of Therapeutics. 2020; 26(2): 113 - 118. DOI: 10.5152/EurJTher.2019.19062
IEEE	Gul Yurtsever S,AYGÜL A,Öztürk İ,nemli s,Kaya S,ERMERTCAN Ş "Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains." European Journal of Therapeutics, 26, ss.113 - 118, 2020. DOI: 10.5152/EurJTher.2019.19062
ISNAD	Gul Yurtsever, Sureyya vd. "Investigation of Various Virulence Factors and SCCmec Types in the Healthcare-associated and Community-associated Methicillin Resistance Staphylococcus aureus Strains". European Journal of Therapeutics 26/2 (2020), 113-118. https://doi.org/DOI: 10.5152/EurJTher.2019.19062