Mustafa DEMIR
(İzmir Katip Çelebi Üniversitesi Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir, Türkiye)
Onur İNCE
(Kütahya Sağlık Bilimleri Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı, Kütahya, Türkiye)
Bulent YILMAZ
(Recep Tayyip Erdoğan Üniversitesi Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Rize, Türkiye)
Mert Ulas BARUT
(Harran Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı, Şanlıurfa, Türkiye)
Ulviye CANSU OZTURK
(Recep Tayyip Erdoğan Üniversitesi Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Rize, Türkiye)
Avni KILIC
(Şanlıurfa Eğitim ve Araştırma Hastanesi Kadın Hastalıkları ve Doğum Kliniği, Şanlıurfa, Türkiye)
Ahmet Berkiz TURP
(Silifke Devlet Hastanesi Kadın Hastalıkları ve Doğum Kliniği, Mersin, Türkiye)
Recep SUTCU
(İzmir Katip Çelebi Üniversitesi Tıp Fakültesi, Biyokimya Anabilim Dalı, İzmir, Türkiye)
SEYİTHAN TAYSI
(Gaziantep Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı, Gaziantep, Türkiye)
Yıl: 2020Cilt: 26Sayı: 2ISSN: 1300-4751 / 2602-4918Sayfa Aralığı: 123 - 129İngilizce

24 0
Oxidative Stress in Endometrial Flushing Fluid of Patients with Polycystic Ovary Syndrome, Endometrioma and Uterine Leiomyoma: Comparison with Healthy Controls
OBJECTIVE: Polycystic ovary syndrome, myoma uteri and endometrioma are frequently seen gynecologic problems and all three diseases may cause infertility. Aim of this cross-sectional study was to compare total antioxidant status, ceruloplasmin, total sulfhydryl , total oxidant status, lipid hydroperoxide and oxidative stress index levels in endometrial flushing fluid of patients with Polycystic ovary syndrome (n=20), uterine leiomyoma (n=20), endometrioma (n=19), and healthy women (n=20). STUDY DESIGN: We compare endometrial flushing fluid of patients with polycystic ovary syndrome (n=20), uterine leiomyoma (n=20), endometrioma (n=19) and healthy women (n=20). Endometrial flushing fluid samples were collected during the implantation window of all women. RESULTS: Mean age of groups was 28.90±5.45, 37.25±2.73, 32.84±6.62 and 32.15±5.18 in Polycystic ovary syndrome, myoma uteri, endometrioma and control groups, respectively (p<0.05). Mean total antioxidant status, ceruloplasmin and total sulfhydryl levels indicating antioxidant state were comparable between Polycystic ovary syndrome, myoma uteri, endometrioma and control groups (p=0.806, p=0.156, p=0.328 respectively for markers). Similarly, oxidant state-related markers didn’t differ significantly between 4 groups (p=0.090 for total oxidant status, p=0.087 for lipid hydroperoxide, p=0.312 for oxidative stress index). CONCLUSION: Endometrial flushing fluid total antioxidant status, total oxidant status, lipid hydroperoxide, ceruloplasmin, and total sulfhydryl levels during implantation window didn’t differ between women with Polycystic ovary syndrome, uterine leiomyoma, endometrioma, and healthy controls.
DergiAraştırma MakalesiErişime Açık
  • 1. Yamamoto Y. Role of active oxygen species and antioxidants in photoaging. J Dermatol Sci. 2001;27 Suppl 1:S1-4.
  • 2. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril. 2003;79(4):829-43.
  • 3. Sharma RK, Agarwal A. Role of reactive oxygen species in gynecologic diseases. Reprod Med Biol. 2004;3(4): 177-199.
  • 4. Agarwal A, Gupta S, Sharma R. Oxidative stress and its implications in female infertility-a clinician's perspective. Reprod Biomed Online. 2005;11(5):641-50.
  • 5. Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress. Curr Biol. 2014;24(10):R453-62.
  • 6. Taysi S, Cikman O, Kaya A, Demircan B, Gumustekin K, Yilmaz A, et al. Increased oxidant stress and decreased antioxidant status in erythrocytes of rats fed with zinc-deficient diet. Biol Trace Elem Res. 2008;123(1-3):161-7.
  • 7. Baysal E, Gulsen S, Aytac I, Celenk F, Ensari N, Taysi S, et al. Oxidative stress in otosclerosis. Redox Rep. 2017;22(5):235-9.
  • 8. Aksoy H, Taysi S, Altinkaynak K, Bakan E, Bakan N, Kumtepe Y. Antioxidant potential and transferrin, ceruloplasmin, and lipid peroxidation levels in women with preeclampsia. J Investig Med. 2003;51(5):284-7.
  • 9. Akinci S, Özcan HC, Balat Ö, Uğur MG, Öztürk E, Taysi S, et al. Assessment of β-hydroxydeoxyguanosine levels in patients with preeclampsia: a prospective study. Clin Exp Obstet Gynecol. 2017;44(2):226-9.
  • 10. Geyik S, Altunisik E, Neyal AM, Taysi S. Oxidative stress and DNA damage in patients with migraine. J Headache Pain. 2016;17:10.
  • 11. Zengin S, Behcet A, Karta S, Can B, Orkmez M, Taskin A, et al. An assessment of antioxidant status in patients with carbon monoxide poisoning. World J Emerg Med. 2014;5(2):91-5.
  • 12. Patel BN, Dunn RJ, Jeong SY, Zhu Q, Julien JP, David S. Ceruloplasmin regulates iron levels in the CNS and prevents free radical injury. J Neurosci. 2002;22(15):657886.
  • 13. Demir M, Ince O, Ozkan B, Kelekci S, Sutcu R, Yilmaz B. Endometrial flushing alphaVbeta3 integrin, glycodelin and PGF2alpha levels for evaluating endometrial receptivity in women with polycystic ovary syndrome, myoma uteri and endometrioma. Gynecol Endocrinol. 2017; 33(9):716-720.
  • 14. Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril. 2004;81(1):19-25.
  • 15. Erel O. Automated measurement of serum ferroxidase activity. Clin Chem. 1998;44(11):2313-9.
  • 16. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70-7.
  • 17. Nourooz-Zadeh J. Ferrous ion oxidation in presence of xylenol orange for detection of lipid hydroperoxides in plasma. Methods Enzymol. 1999;300:58-62.
  • 18. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12): 110311.
  • 19. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37(4): 277-85.
  • 20. Harma M, Harma M, Erel O. Oxidative stress in women with preeclampsia. Am J Obstet Gynecol. 2005;192(2): 656-7;author reply 657.
  • 21. Pasqualotto EB, Agarwal A, Sharma RK, Izzo VM, Pinotti JA, Joshi NJ, et al. Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures. Fertil Steril. 2004;81(4):973-6.
  • 22. Yeon Lee J, Baw C-K, Gupta S, Aziz N, Agarwal A. Role of oxidative stress in polycystic ovary syndrome. Current Women's Health Reviews. 2010;2(6):96-107.
  • 23. Hilali N, Vural M, Camuzcuoglu H, Camuzcuoglu A, Aksoy N. Increased prolidase activity and oxidative stress in PCOS. Clin Endocrinol (Oxf). 2013;79(1):105-10.
  • 24. Zuo T, Zhu M, Xu W. Roles of oxidative stress in polycystic ovary syndrome and cancers. Oxid Med Cell Longev. 2016;2016:8589318.35.
  • 25. Torun AN, Vural M, Cece H, Camuzcuoglu H, Toy H, Aksoy N. Paraoxonase-1 is not affected in polycystic ovary syndrome without metabolic syndrome and insulin resistance, but oxidative stress is altered. Gynecol Endocrinol. 2011;27(12):988-92.36.
  • 26. Zhang R, Liu H, Bai H, Zhang Y, Liu Q, Guan L, et al. et al. Oxidative stress status in Chinese women with different clinical phenotypes of polycystic ovary syndrome. Clin Endocrinol (Oxf). 2017;86(1):88-96.
  • 27. Turan V, Sezer ED, Zeybek B, Sendag F. Infertility and the presence of insulin resistance are associated with increased oxidative stress in young, non-obese Turkish women with polycystic ovary syndrome. J Pediatr Adolesc Gynecol. 2015;28(2):119-23.
  • 28. Jackson LW, Schisterman EF, Dey-Rao R, Browne R, Armstrong D. Oxidative stress and endometriosis. Hum Reprod. 2005;20(7):2014-20.
  • 29. Bedaiwy MA, Falcone T, Sharma RK, Goldberg JM, Attaran M, Nelson DR, et al. Prediction of endometriosis with serum and peritoneal fluid markers: a prospective controlled trial. Hum Reprod. 2002;17(2):426-31.
  • 30. Wang Y, Sharma RK, Falcone T, Goldberg J, Agarwal A. Importance of reactive oxygen species in the peritoneal fluid of women with endometriosis or idiopathic infertility. Fertil Steril. 1997;68(5):826-30.
  • 31. Turkyilmaz E, Yildirim M, Cendek BD, Baran P, Alisik M, Dalgaci F, et al. Evaluation of oxidative stress markers and intra-extracellular antioxidant activities in patients with endometriosis. Eur J Obstet Gynecol Reprod Biol. 2016;199:164-8.
  • 32. Turgut A, Özler A, Görük NY, Tunc SY, Evliyaoglu O, Gül T. Copper, ceruloplasmin and oxidative stress in patients with advanced-stage endometriosis. Eur Rev Med Pharmacol Sci. 2013;17(11):1472-8.
  • 33. Oner-Iyidoğan Y1, Koçak H, Gürdöl F, Korkmaz D, Buyru F. Indices of oxidative stress in eutopic and ectopic endometria of women with endometriosis. Gynecol Obstet Invest. 2004;57(4):214-7.
  • 34. Van Langendonckt A, Casanas-Roux F, Donnez J. Oxidative stress and peritoneal endometriosis. Fertil Steril. 2002;77(5):861-70.48.
  • 35. Rackow BW, Taylor HS. Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity. Fertil Steril. 2010;93(6):2027-34.
  • 36. Pejić S, Todorović A, Stojiljković V, Gavrilović L, Popović N, Pajović SB. Antioxidant status in women with uterine leiomyoma: relation with sex hormones. An Acad Bras Cienc. 2015 Sep;87(3):1771-82.
  • 37. Pejić S, Todorović A, Stojiljković V, Kasapović J, Pajović SB. Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma. Reprod Biol Endocrinol. 2009; 7:149.
  • 38. Ghiselli A, Serafini M, Natella F, Scaccini C. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic Biol Med. 2000; 29(11):1106-14.
  • 39. Pejić S, Todorović A, Stojiljković V, Cvetković D, Lucić N, Radojicić RM, et al. Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium. An Acad Bras Cienc. 2008;80(3):515-22.
  • 40. Nayki C, Nayki U, Gunay M, Kulhan M, Cankaya M, Humeyra Taskin Kafa A, et al. Oxidative and antioxidative status in the endometrium of patients with benign gynecological disorders. J Gynecol Obstet Hum Reprod. 2017; 46(3):243-7.

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