Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods

ÜTÜK, Armağan Erdem; GÜNAYDIN, Elçin; sezer, osman; guven gokmen, tulin; AYVAZOĞLU DEMİR, Pınar

Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods

Tülin GÜVEN GÖKMEN, (Çukurova Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Adana, Türkiye)

Elçin GÜNAYDIN, (Hitit Üniversitesi, Alaca Avni Çelik Meslek Yüksekokulu, Çorum, Türkiye)

Osman SEZER, (Adana Veteriner Kontrol Enstitüsü, Parazitoloji Laboratuvarı, Adana, Türkiye)

Pınar AYVAZOĞLU DEMİR, (Kafkas Üniversitesi, Veteriner Fakültesi, Hayvancılık Ekonomisi Anabilim Dalı, Kars, Türkiye)

Armağan Erdem ÜTÜK (Çukurova Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Adana, Türkiye)

Etlik Veteriner Mikrobiyoloji Dergisi

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Yıl: 2019 Cilt: 30 Sayı: 2 Sayfa Aralığı: 109 - 114 Metin Dili: İngilizce İndeks Tarihi: 27-10-2020

Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods

Öz:

Anaplasmosis is a common disease in tropical and subtropical climate zone and is transmitted by vectors.Especially in large cattle management systems, it has started to be detected frequently in recent years. The aim ofthis study was to determine the prevalence of Anaplasma spp. in cattle in Adana province. For this aim, 187 bloodsamples were collected from cattle from fifteen districts of Adana that have different climatic zones and examined byCompetitive ELISA (cELISA) and Nested-PCR methods. Seropositivity was determined as 38.5% (72/187) in cattle.The molecular prevalence was detected as 1.6% (3/187) for Anaplasma centrale and 3.2% (6/187) for Anaplasmamarginale by Nested-Polymerase Chain Reaction (PCR) methods. In this study, epidemiological data related to bovineanaplasmosis in Adana province of Turkey were discussed in detail and it was thought that the obtained data wouldcontribute to disease prevention and control programs.

Anahtar Kelime:

Adana İlinde Sığırlarda Anaplasma marginale ve Anaplasma centrale’nin Serolojik ve Moleküler Yöntemler ile Araştırılması

Öz:

Anaplazmozis tropikal ve subtropikal iklim bölgelerinde vektörler tarafından taşınan yaygın bir hastalıktır. Özellikle büyükbaş hayvancılık işletmelerinde son yıllarda sık tespit edilmeye başlanmıştır. Bu çalışmanın amacı, Adana ilindeki sığırlarda Anaplasma spp. prevalansını belirlemekti. Bu amaçla Adana’nın farklı iklim bölgelerine sahip on beş ilçesinde sığırlardan 187 kan örneği alınarak kompetetif ELISA (cELISA) ve Nested-PCR yöntemleriyle incelenmiştir. Sığırlarda seropozitiflik %38,5 (72/187) olarak belirlenmiştir. Ayrıca moleküler prevalans, Nested-PCR yöntemleriyle Anaplasma centrale için %1,6 (3/187), Anaplasma marginale için %3,2 (6/187) olarak tespit edilmiştir. Bu çalışma ile Türkiye’nin Adana ilinde sığır anaplazmozisi ile ilgili epidemiyolojik verileri ayrıntılı bir şekilde ele alınmış ve elde edilen verilerin hastalığın önlenmesine ve kontrol programlarına katkıda bulunacağı düşünülmüştür.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	guven gokmen t, GÜNAYDIN E, sezer o, AYVAZOĞLU DEMİR P, ÜTÜK A (2019). Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. , 109 - 114.
Chicago	guven gokmen tulin,GÜNAYDIN Elçin,sezer osman,AYVAZOĞLU DEMİR Pınar,ÜTÜK Armağan Erdem Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. (2019): 109 - 114.
MLA	guven gokmen tulin,GÜNAYDIN Elçin,sezer osman,AYVAZOĞLU DEMİR Pınar,ÜTÜK Armağan Erdem Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. , 2019, ss.109 - 114.
AMA	guven gokmen t,GÜNAYDIN E,sezer o,AYVAZOĞLU DEMİR P,ÜTÜK A Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. . 2019; 109 - 114.
Vancouver	guven gokmen t,GÜNAYDIN E,sezer o,AYVAZOĞLU DEMİR P,ÜTÜK A Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. . 2019; 109 - 114.
IEEE	guven gokmen t,GÜNAYDIN E,sezer o,AYVAZOĞLU DEMİR P,ÜTÜK A "Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods." , ss.109 - 114, 2019.
ISNAD	guven gokmen, tulin vd. "Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods". (2019), 109-114.

APA	guven gokmen t, GÜNAYDIN E, sezer o, AYVAZOĞLU DEMİR P, ÜTÜK A (2019). Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. Etlik Veteriner Mikrobiyoloji Dergisi, 30(2), 109 - 114.
Chicago	guven gokmen tulin,GÜNAYDIN Elçin,sezer osman,AYVAZOĞLU DEMİR Pınar,ÜTÜK Armağan Erdem Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. Etlik Veteriner Mikrobiyoloji Dergisi 30, no.2 (2019): 109 - 114.
MLA	guven gokmen tulin,GÜNAYDIN Elçin,sezer osman,AYVAZOĞLU DEMİR Pınar,ÜTÜK Armağan Erdem Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. Etlik Veteriner Mikrobiyoloji Dergisi, vol.30, no.2, 2019, ss.109 - 114.
AMA	guven gokmen t,GÜNAYDIN E,sezer o,AYVAZOĞLU DEMİR P,ÜTÜK A Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. Etlik Veteriner Mikrobiyoloji Dergisi. 2019; 30(2): 109 - 114.
Vancouver	guven gokmen t,GÜNAYDIN E,sezer o,AYVAZOĞLU DEMİR P,ÜTÜK A Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods. Etlik Veteriner Mikrobiyoloji Dergisi. 2019; 30(2): 109 - 114.
IEEE	guven gokmen t,GÜNAYDIN E,sezer o,AYVAZOĞLU DEMİR P,ÜTÜK A "Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods." Etlik Veteriner Mikrobiyoloji Dergisi, 30, ss.109 - 114, 2019.
ISNAD	guven gokmen, tulin vd. "Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods". Etlik Veteriner Mikrobiyoloji Dergisi 30/2 (2019), 109-114.