Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study

Gündüz, Reyhan; UGUR, Mete Gurol; özcan, Hüseyin çağlayan; Taysı, Seyithan; bayramoğlu tepe, neslihan; Sari demir, Sevgi; balat, ozcan

doi:10.5798/dicletip.706022

Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study

Reyhan GÜNDÜZ, (Dicle Üniversitesi, Tıp Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı, Diyarbakır, Türkiye)

Mete Gürol UĞUR, (Gaziantep Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Gaziantep, Türkiye)

Neslihan TEPE, (Gaziantep Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Gaziantep, Türkiye)

Hüseyin ÖZCAN, (Gaziantep Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Gaziantep, Türkiye)

Özcan BALAT, (Gaziantep Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Gaziantep, Türkiye)

Sevgi SARI DEMİR, (Adana Medline Hastanesi, Kadın Hastalıkları ve Doğum Kliniği, Adana, Türkiye)

SEYİTHAN TAYSI (Gaziantep Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Gaziantep, Türkiye)

Dicle Tıp Dergisi

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Yıl: 2020 Cilt: 47 Sayı: 1 Sayfa Aralığı: 74 - 81 Metin Dili: İngilizce DOI: 10.5798/dicletip.706022 İndeks Tarihi: 29-10-2020

Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study

Öz:

Objective: This study was aimed to evaluate 8-hydroxy-2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levelsin patients with first trimester miscarriage.Methods: This prospective cohort study included 35 women, who were diagnosed with miscarriage (patient group) and35 healthy pregnant women below 14 weeks of gestation (control group), who did not report any complaints. 8-OHdGlevels were measured with a competitive ELISA (Enzyme-Linked ImmunoSorbent Assay) high sensitivity kit and MDAlevels were also measured in both groups.Results: There wasn’t difference in terms of complete blood count, thyroid-stimulating hormone (TSH), homocysteinevalues, parity, age, body mass index (BMI) and gestational age between the groups. Serum 8-OHdG and MDA levels weresignificantly higher in the patient group (p=0.001). A moderate positive significant correlation between the gestationalweek and MDA values and a weak but significant positive correlation between 8-OHdG values and gestational week weredetected in the patient group.Conclusion: 8-OHdG and MDA could play a role in the etiopathogenesis of first-trimester miscarriage. However, this rolemust be further delineated in large-scaled studies for early diagnosis and management of this condition.

Anahtar Kelime:

Missed Abortuslarda 8-Hidroksi-2-Deoksiguanozin Ve Malondialdehit Seviyelerinin Değerlendirilmesi: Prospektif Kohort Çalışması

Öz:

Amaç: Bu çalışma missed abortusu olan hastalarda 8-hidroksi-2-deoksiguanozin (8-OHdG) ve malondialdehit (MDA) seviyelerini değerlendirmeği amaçladı. Metods: Bu prospektif kohort çalışmasına missed abortus tanısı alan 35 kadın (hasta grubu) ve herhangi bir şikayeti olmayan 14 hafta altı 35 sağlıklı gebe kadın (kontrol grubu) dahil edildi. 8- OHdG seviyeleri yarışmalı sensitivitesi yüksek ELISA (Enzyme-Linked ImmunoSorbent Assay) kiti ile ölçüldü ve MDA seviyeleri de her iki grupta ölçüldü. Bulgular: Gruplar arasında tam kan sayımı, tiroid stimulan hormon (TSH), homosistein değerleri, parite, yaş, vücut kitle indeksi (VKİ) ve gebelik yaşı açısından fark yoktu. Hasta grubunda serum 8-OHdG ve MDA düzeyleri önemli ölçüde daha yüksek bulundu (p=0.001). Hasta grubunda, gebelik haftası ile MDA değerleri arasında orta derecede pozitif anlamlı bir korelasyon vardı ve 8-OHdG değerleri ve gebelik haftası arasında zayıf ama anlamlı pozitif bir korelasyon olduğu tespit edildi. Sonuç: 8-OHdG ve MDA missed abortus etiyopatogenezinde rol oynayabilir. Ancak, bu durumun erken teşhis ve tedavisi için geniş ölçekli çalışmalarda bu rol daha fazla tanımlanmalıdır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Cunningham FG, Leveno KJ, Bloom SL, et al. Williams Obstetrics, 24 th ed. NewYork: McGraw-Hill, 2014.
2. Simpson JL, Carson SA. Causes of fetal loss. In; Gray R, Leridon L, Spira F (eds). Symposium on Biological and Demographic Determinants of Human Reproduction. Oxford, Oxford University. 1993; 287.
3. Gergerlioglu HS, Savas HA, Bulbul F, et al. Changes in nitric oxide level and superoxide dismutase activity during antimanic treatment. Prog Neuropsychopharmacol Biol Psychiatry. 2007; 31: 697-702.
4. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemicobiological interactions. 2006; 160: 1-40.
5. Long JD, Matson WR, Juhl AR, Leavitt BR, Paulsen JS. PREDICT-HD Investigators and Coordinators of the Huntington Study Group. 8OHdG as a marker for Huntington disease progression. Neurobiology of Disease. 2012; 625-34.
6. Kaçmaz A, Polat A, User Y, et al. Octreotide improves reperfusion-induced oxidative injury in acute abdominal hypertension in rats. J Gastrointest Surg. 2004; 113-9.
7. Okutan H, Savas C, Delibas N. The antioxidant effect of melatonin in lung injury after aortic occlusion-reperfusion. Interact CardiovascThorac Surg. 2004; 3: 519-22.
8. Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: Reference interval and effects of life-style factors. Clin Chem. 1997; 43: 1209-14.
9. Altuntaş H, Bayram F, Bitgen N, et al. Increased Chromosomal and Oxidative DNA Damage in Patients with Multinodular Goiter and Their Association with Cancer. Int J Endocrinol. 2017; Article ID 2907281.
10. Hunter MI, Nlemadim BC, Davidson DL. Lipid peroxidation products and antioxidant proteins in plasma and cerebrospinal fluid from multiple sclerosis patients. Neurochem Res. 1985; 10: 1645-52.
11. Schorge JO, Schaffer JI, Halvorson LM, et al. Williams Gynecology, 2nd ed. NewYork: McGraw-Hill, 2010.
12. Li TC, Makris M, Tomsu M, Tuckerman E, Laird S. Recurrent miscarriage: aetiology, management, and prognosis. Hum Reprod Update. 2002; 8: 463-81.
13. Wilcox AJ, Weinberg CR, O'Connor JF, et al. Incidence of early loss of pregnancy. N Engl J Med. 1988; 319: 189-94.
14. Maisonneuve E, Delvin E, Ouellet A, et al. Oxidative conditions prevail in severe IUGR with vascular disease and Doppler anomalies. J Matern Fetal Neonatal Med. 2015; 28: 1471-5.
15. Atiba AS, Abbiyesuku FM, Adekanle DA, et al. Malondialdehyde and antioxidant enzymes in second and third trimesters of pre-eclamptic Nigerian women. Niger Postgrad Med J. 2014; 21: 150-4.
16. Ferguson KK, McElrath TF, Chen YH, et al. Repeated measures of urinary oxidative stress biomarkers during pregnancy and preterm birth. Am J Obstet Gynecol. 2015; 212: 208.
17. Negi R, Pande D, Kumar A, Khanna RS, Khanna HD. In vivo oxidative DNA damage and lipid peroxidation as a biomarker of oxidative stress in preterm low-birthweight infants. J Trop Pediatr. 2012; 58: 326-8.
18. Qiu C, Hevner K, Abetew D, Enquobahrie DA, Williams MA. Oxidative DNA damage in early pregnancy and risk of gestational diabetes mellitus: A pilot study. Clinical biochemistry. 2011; 44: 804-8.
19. Peter Stein T, Scholl TO, Schluter MD, et al. Oxidative stress early in pregnancy and pregnancy outcome. Free radical research. 2008; 42: 841-8.
20. Mol BW, Lijmer JG, Ankum WM, Van der Veen F, Bossuyt PM. The accuracy of single serum progesterone measurement in the diagnosis of ectopic pregnancy: a metaanalysis. Hum Reprod Nov. 1998; 13: 3220-7.
21. Onan A, Kurdoglu M, Sancak B, Bukan N, Yildirim M. Lipid peroxidation in nuchal cord cases: implication for fetal distress. J Matern Fetal Neonatal Med. 2009; 22: 254-8.
22. Singh AK, Pandey P, Tewari M, et al. Free radicals hasten head and neck cancer risk: A study of total oxidant, total antioxidant, DNA damage, and histological grade. J Postgrad Med. 2016; 62: 96–101.

APA	Gündüz R, UGUR M, bayramoğlu tepe n, özcan H, balat o, Sari demir S, Taysı S (2020). Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. , 74 - 81. 10.5798/dicletip.706022
Chicago	Gündüz Reyhan,UGUR Mete Gurol,bayramoğlu tepe neslihan,özcan Hüseyin çağlayan,balat ozcan,Sari demir Sevgi,Taysı Seyithan Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. (2020): 74 - 81. 10.5798/dicletip.706022
MLA	Gündüz Reyhan,UGUR Mete Gurol,bayramoğlu tepe neslihan,özcan Hüseyin çağlayan,balat ozcan,Sari demir Sevgi,Taysı Seyithan Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. , 2020, ss.74 - 81. 10.5798/dicletip.706022
AMA	Gündüz R,UGUR M,bayramoğlu tepe n,özcan H,balat o,Sari demir S,Taysı S Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. . 2020; 74 - 81. 10.5798/dicletip.706022
Vancouver	Gündüz R,UGUR M,bayramoğlu tepe n,özcan H,balat o,Sari demir S,Taysı S Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. . 2020; 74 - 81. 10.5798/dicletip.706022
IEEE	Gündüz R,UGUR M,bayramoğlu tepe n,özcan H,balat o,Sari demir S,Taysı S "Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study." , ss.74 - 81, 2020. 10.5798/dicletip.706022
ISNAD	Gündüz, Reyhan vd. "Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study". (2020), 74-81. https://doi.org/10.5798/dicletip.706022

APA	Gündüz R, UGUR M, bayramoğlu tepe n, özcan H, balat o, Sari demir S, Taysı S (2020). Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. Dicle Tıp Dergisi, 47(1), 74 - 81. 10.5798/dicletip.706022
Chicago	Gündüz Reyhan,UGUR Mete Gurol,bayramoğlu tepe neslihan,özcan Hüseyin çağlayan,balat ozcan,Sari demir Sevgi,Taysı Seyithan Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. Dicle Tıp Dergisi 47, no.1 (2020): 74 - 81. 10.5798/dicletip.706022
MLA	Gündüz Reyhan,UGUR Mete Gurol,bayramoğlu tepe neslihan,özcan Hüseyin çağlayan,balat ozcan,Sari demir Sevgi,Taysı Seyithan Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. Dicle Tıp Dergisi, vol.47, no.1, 2020, ss.74 - 81. 10.5798/dicletip.706022
AMA	Gündüz R,UGUR M,bayramoğlu tepe n,özcan H,balat o,Sari demir S,Taysı S Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. Dicle Tıp Dergisi. 2020; 47(1): 74 - 81. 10.5798/dicletip.706022
Vancouver	Gündüz R,UGUR M,bayramoğlu tepe n,özcan H,balat o,Sari demir S,Taysı S Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study. Dicle Tıp Dergisi. 2020; 47(1): 74 - 81. 10.5798/dicletip.706022
IEEE	Gündüz R,UGUR M,bayramoğlu tepe n,özcan H,balat o,Sari demir S,Taysı S "Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study." Dicle Tıp Dergisi, 47, ss.74 - 81, 2020. 10.5798/dicletip.706022
ISNAD	Gündüz, Reyhan vd. "Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study". Dicle Tıp Dergisi 47/1 (2020), 74-81. https://doi.org/10.5798/dicletip.706022