The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease

Sucu, Seyhun; balat, ozcan; bademkıran, muhammed hanifi; özcan, Hüseyin çağlayan; obut, mehmet; CEBESOY, fatma bahar

doi:10.4274/tjod.galenos.2019.54815

The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease

Muhammet Hanifi BADEMKIRAN, (Diyarbakır Sağlık Bilimleri Üniversitesi, Gazi Yaşargil Eğitim ve Araştırma Hastanesi, Kadın Hastalıkları ve Doğum Kliniği, Diyarbakır, Türkiye)

Özcan BALAT, (Gaziantep Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Gaziantep, Türkiye)

Seyhun SUCU, (Gaziantep Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Gaziantep, Türkiye)

Mehmet OBUT, (Diyarbakır Sağlık Bilimleri Üniversitesi, Gazi Yaşargil Eğitim ve Araştırma Hastanesi, Kadın Hastalıkları ve Doğum Kliniği, Diyarbakır, Türkiye)

Hüseyin ÖZCAN, (Gaziantep Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Gaziantep, Türkiye)

Fatma Bahar CEBESOY (Özel Muayenehane, Kadın Hastalıkları ve Doğum Kliniği, Gaziantep, Türkiye)

Turkish Journal of Obstetrics and Gynecology

0 0

Yıl: 2019 Cilt: 16 Sayı: 4 Sayfa Aralığı: 249 - 254 Metin Dili: İngilizce DOI: 10.4274/tjod.galenos.2019.54815 İndeks Tarihi: 30-10-2020

The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease

Öz:

Objective: To study cell-free DNA (cfDNA) levels in patients with gestational trophoblastic disease (GTD) in order to test the hypothesis that cfDNA circulating inmaternal plasma could provide early detection of GTD.Materials and Methods: This study included 32 patients with GTD (complete mole and partial mole) and 30 non-GTD patients in the first trimester of pregnancywith no other medical problems. cfDNA levels in maternal serum were measured using polymerase chain reaction analysis on Y-chromosome–specific sequences.Results: cfDNA was found as 327±367 pg on average in the control group and 600±535 pg in the GTD group. Within the GTD group, the partial mole grouphad an cfDNA average of 636±549 pg, and the complete mole group had an cfDNA average of 563±536 pg. Although there was a statistically significant differencebetween the GTD group and the control group in terms of cfDNA (p=0.02), there was no statistically significant difference between the complete mole group andthe partial mole group (p=0.76).Conclusion: Non-parametric analysis of covariance in terms of cfDNA in GTD was performed, thereby increasing its power and revealing a significant differencecompared with the control group. This indicates that maternal peripheral bloodstream cfDNA monitoring might be significant in the early diagnosis of GTD.

Anahtar Kelime:

Gestasyonal trofoblastik hastalığın erken tanısında serum fetal DNA’nın potansiyeli

Öz:

Amaç: Maternal plazmada dolaşan serum fetal DNA’nın (cfDNA) gestasyonel trofoblastik hastalığın (GTH) erken tanısında potansiyeli olduğunu tespit etmek için GTH’de cfDNA düzeylerini inceledik. Gereç ve Yöntemler: Çalışmaya, gebeliğin ilk üç ayında diğer tıbbi problemleri olmayan 32 GTH hastası (komplet mol ve inkomplet mol) ve GTH olmayan 30 hasta dahil edildi. Maternal serumdaki cfDNA seviyeleri, Y-kromozomuna spesifik dizinlerde polimeraz zincir reaksiyonu analizi kullanılarak ölçüldü. Bulgular: Kontrol grubunda cfDNA ortalama olarak 327±367 pg, GTH grubunda 600±535 pg olarak bulundu. GTH grubunda Inkomplet mol grubu cfDNA ortalaması 636±549 pg idi ve komplet mol grubu cfDNA ortalaması 563±536 pg idi. GTH grubu ile kontrol grubu arasında istatistiksel olarak anlamlı bir fark vardı (p=0,02). cfDNA açısından, komplet mol grubu ile inkomplet mol grubu arasında istatistiksel olarak anlamlı bir fark bulunmadı (p=0,76). Sonuç: GTH’de cfDNA açısından kontrol grubuyla karşılaştırıldığında anlamlı bir fark ortaya çıktı. Bu maternal periferik kan akımı cfDNA izlemenin GTH’nin erken tanısında önemli olabileceğini göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Coletta JM, Hou JY. 104-Gestational Trophoblastic Disease. Second Edi. Obstetric Imaging: Fetal Diagnosis and Care: Second Edition. Elsevier Inc.; 2017.449-452.e1 s.
2. Flamini E, Mercatali L, Nanni O, Calistri D, Nunziatini R, Zoli W, et al. Free DNA and carcinoembryonic antigen serum levels: An important combination for diagnosis of colorectal cancer. Clin Cancer Res 2006;12:6985-8.
3. Xue X, Teare MD, Holen I, Zhu YM, Woll PJ. Optimizing the yield and utility of circulating cell-free DNA from plasma and serum. Clin Chim Acta 2009;404:100-4.
4. Wichmann D, Panning M, Quack T, Kramme S, Burchard G-D, Grevelding C, et al. Diagnosing Schistosomiasis by Detection of Cell-Free Parasite DNA in Human Plasma. Correa-Oliveira R, editör. PLoS Negl Trop Dis 2009;3:e422.
5. Litton C, Stone J, Eddleman K, Lee M-J. Noninvasive Prenatal Diagnosis: Past, Present, and Future. Mt Sinai J Med A J Transl Pers Med 2009;76:521-8.
6. Lazar L, Rigó J, Nagy B, Balogh K, Makó V, Cervenak L, et al. Relationship of circulating cell-free DNA levels to cell-free fetal DNA levels, clinical characteristics and laboratory parameters in preeclampsia. BMC Med Genet 2009;10:120.
7. Lo YMD, Leung TN, Tein MSC, Sargent IL, Zhang J, Lau TK, et al. Quantitative abnormalities of fetal DNA in maternal serum in preeclampsia. Clin Chem 1999;45:184-8.
8. Zhang J, Lau TK, Leung TN, Lo YMD, Hjelm NM. Maternal plasma fetal DNA as a marker for preterm labour. Lancet 2005;352:1904-5.
9. González-González MC, Trujillo MJ, Rodríguez de Alba M, GarcíaHoyos M, Lorda-Sánchez I, Díaz-Recasens J, et al. Huntington disease-unaffected fetus diagnosed from maternal plasma using QFPCR. Prenat Diagn 2003;23:232-4.
10. Chiu RWK, Lau TK, Cheung PT, Gong ZQ, Leung TN, Lo YMD. Noninvasive prenatal exclusion of congenital adrenal hyperplasia by maternal plasma analysis: a feasibility study. Clin Chem 2002;48:778-80.
11. Hardison RC, Chui DHK, Giardine B, Riemer C, Patrinos GP, Anagnou N, et al. HbVar: A relational database of human hemoglobin variants and thalassemia mutations at the globin gene server. Hum Mutat 2002;19:225-33.
12. Sekizawa A, Jimbo M, Saito H, Iwasaki M, Sugito Y, Yukimoto Y, et al. Increased cell-free fetal DNA in plasma of two women with invasive placenta. Clin Chem 2002;48:353-4.
13. Sekizawa A, Sugito Y, Iwasaki M, Watanabe A, Jimbo M, Hoshi S, et al. Cell-free fetal DNA is increased in plasma of women with hyperemesis gravidarum. Clin Chem 2001;47:2164-5.
14. Caramelli E, Rizzo N, Concu M, Simonazzi G, Carinci P, Bondavalli C, et al. Cell-free fetal DNA concentration in plasma of patients with abnormal uterine artery Doppler waveform and intrauterine growth restriction--a pilot study. Prenat Diagn 2003;23:367-71.
15. Lau TK, Lo KW, Chan LYS, Leung TY, Lo YM. Cell-free fetal deoxyribonucleic acid in maternal circulation as a marker of fetalmaternal hemorrhage in patients undergoing external cephalic version near term. Am J Obstet Gynecol 2000;183:712-6.
16. Zhong XY, Holzgreve W, Li JC, Aydinli K, Hahn S. High levels of fetal erythroblasts and fetal extracellular DNA in the peripheral blood of a pregnant woman with idiopathic polyhydramnios: case report. Prenat Diagn 2000;20:838-41.
17. Saito H, Sekizawa A, Morimoto T, Suzuki M, Yanaihara T. Prenatal DNA diagnosis of a single-gene disorder from maternal plasma. Lancet 2000;356:1170.
18. González-González MC, García-Hoyos M, Trujillo MJ, Rodríguez de Alba M, Lorda-Sánchez I, Díaz-Recasens J, et al. Prenatal detection of a cystic fibrosis mutation in fetal DNA from maternal plasma. Prenat Diagn 2002;22:946-8.
19. Amicucci P, Gennarelli M, Novelli G, Dallapiccola B. Prenatal diagnosis of myotonic dystrophy using fetal DNA obtained from maternal plasma. Clin Chem 2000;46:301-2.
20. Tjoa ML, Cindrova-Davies T, Spasic-Boskovic O, Bianchi DW, Burton GJ. Trophoblastic oxidative stress and the release of cell-free feto-placental DNA. Am J Pathol. Ağustos 2006;169:400-4.
21. Alberry M, Maddocks D, Jones M, Abdel Hadi M, Abdel-Fattah S, Avent N, et al. Free fetal DNA in maternal plasma in anembryonic pregnancies: confirmation that the origin is the trophoblast. Prenat Diagn 2007;27:415-8.
22. Sifakis S, Zaravinos A, Maiz N, Spandidos DA, Nicolaides KH. Firsttrimester maternal plasma cell-free fetal DNA and preeclampsia. Am J Obstet Gynecol 2009;201:472.e1-7.
23. Yin A, Ng EHY, Zhang X, He Y, Wu J, Leung KY. Correlation of maternal plasma total cell-free DNA and fetal DNA levels with short term outcome of first-trimester vaginal bleeding. Hum Reprod 2007;22:1736-43.
24. Taglauer ES, Wilkins-Haug L, Bianchi DW. Review: Cell-free fetal DNA in the maternal circulation as an indication of placental health and disease. Placenta 2014;35:S64-8.
25. Openshaw MR, Harvey RA, Sebire NJ, Kaur B, Sarwar N, Seckl MJ, et al. Circulating Cell Free DNA in the Diagnosis of Trophoblastic Tumors. EBioMedicine 2016;4:146-52.
26. Zeybek YG, Günel T, Benian A, Aydınlı K, Kaleli S. Clinical evaluations of cell-free fetal DNA quantities in pre-eclamptic pregnancies. J Obstet Gynaecol Res 2013;39:632-40.
27. Karuserci ÖK, Uğur MG, Balat Ö, Sucu S. Ektopik gebelik tanısında serbest fetal DNA’nın yeri: ön hazırlık çalışması. Turk Jinekoloji ve Obstet Dern Derg 2017;14:156-9.

APA	bademkıran m, balat o, Sucu S, obut m, özcan H, CEBESOY f (2019). The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. , 249 - 254. 10.4274/tjod.galenos.2019.54815
Chicago	bademkıran muhammed hanifi,balat ozcan,Sucu Seyhun,obut mehmet,özcan Hüseyin çağlayan,CEBESOY fatma bahar The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. (2019): 249 - 254. 10.4274/tjod.galenos.2019.54815
MLA	bademkıran muhammed hanifi,balat ozcan,Sucu Seyhun,obut mehmet,özcan Hüseyin çağlayan,CEBESOY fatma bahar The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. , 2019, ss.249 - 254. 10.4274/tjod.galenos.2019.54815
AMA	bademkıran m,balat o,Sucu S,obut m,özcan H,CEBESOY f The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. . 2019; 249 - 254. 10.4274/tjod.galenos.2019.54815
Vancouver	bademkıran m,balat o,Sucu S,obut m,özcan H,CEBESOY f The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. . 2019; 249 - 254. 10.4274/tjod.galenos.2019.54815
IEEE	bademkıran m,balat o,Sucu S,obut m,özcan H,CEBESOY f "The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease." , ss.249 - 254, 2019. 10.4274/tjod.galenos.2019.54815
ISNAD	bademkıran, muhammed hanifi vd. "The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease". (2019), 249-254. https://doi.org/10.4274/tjod.galenos.2019.54815

APA	bademkıran m, balat o, Sucu S, obut m, özcan H, CEBESOY f (2019). The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. Turkish Journal of Obstetrics and Gynecology, 16(4), 249 - 254. 10.4274/tjod.galenos.2019.54815
Chicago	bademkıran muhammed hanifi,balat ozcan,Sucu Seyhun,obut mehmet,özcan Hüseyin çağlayan,CEBESOY fatma bahar The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. Turkish Journal of Obstetrics and Gynecology 16, no.4 (2019): 249 - 254. 10.4274/tjod.galenos.2019.54815
MLA	bademkıran muhammed hanifi,balat ozcan,Sucu Seyhun,obut mehmet,özcan Hüseyin çağlayan,CEBESOY fatma bahar The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. Turkish Journal of Obstetrics and Gynecology, vol.16, no.4, 2019, ss.249 - 254. 10.4274/tjod.galenos.2019.54815
AMA	bademkıran m,balat o,Sucu S,obut m,özcan H,CEBESOY f The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. Turkish Journal of Obstetrics and Gynecology. 2019; 16(4): 249 - 254. 10.4274/tjod.galenos.2019.54815
Vancouver	bademkıran m,balat o,Sucu S,obut m,özcan H,CEBESOY f The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease. Turkish Journal of Obstetrics and Gynecology. 2019; 16(4): 249 - 254. 10.4274/tjod.galenos.2019.54815
IEEE	bademkıran m,balat o,Sucu S,obut m,özcan H,CEBESOY f "The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease." Turkish Journal of Obstetrics and Gynecology, 16, ss.249 - 254, 2019. 10.4274/tjod.galenos.2019.54815
ISNAD	bademkıran, muhammed hanifi vd. "The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease". Turkish Journal of Obstetrics and Gynecology 16/4 (2019), 249-254. https://doi.org/10.4274/tjod.galenos.2019.54815