Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus

ÇINAR, Miyase; ERAT, Serkan; Macun, Hasan Ceyhun; KALENDER, Hakan; KÜRŞAT AZKUR, Ahmet

doi:10.17094/ataunivbd.315776

Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus

Hasan Ceyhun MACUN, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Kırıkkale, Türkiye)

Miyase ÇINAR, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kırıkkale, Türkiye)

Ahmet Kürşat AZKUR, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Kırıkkale, Türkiye)

Hakan KALENDER, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Kırıkkale, Türkiye)

Serkan ERAT (Kırıkkale Üniversitesi, Veteriner Fakültesi, Hayvancılık Anabilim Dalı, Kırıkkale, Türkiye)

Atatürk Üniversitesi Veteriner Bilimleri Dergisi

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Yıl: 2018 Cilt: 13 Sayı: 2 Sayfa Aralığı: 128 - 134 Metin Dili: İngilizce DOI: 10.17094/ataunivbd.315776 İndeks Tarihi: 01-11-2020

Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus

Öz:

The aim of this study was to determine oxidant-antioxidant status by evaluating some oxidative stress parameters(nitric oxide (NO), malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), catalase activity) inclinically healthy Akkaraman ewes with seropositive for Schmallenberg virus (SBV). The study consisted of four positive, sixsuspected and seven negative-control blood sera in terms of specific antibody for SBV by using commercial ELISA kits. Eventhough there were no significant statistical differences (P>0.05), the highest NO level was in sera with SBV specific antibodypositive while the lowest TOS level wasin sera with SBV specific antibody negative. The values of TAS in sera were very similarin the groups (P=0.981). While the MDA levels of sera with SBV specific antibody positive and suspected were similar, thestatistical difference was found between the MDA level of sera with SBV specific antibody positive and negative (P=0.021).The catalase activity of sera with SBV specific antibody positive was lower (P=0.030) than the catalase activity of sera withSBV suspected whereas the catalase activities in positive and negative groups and the catalase activities in suspected andnegative groups were similar. In conclusion, it was determined that MDA level was affected in Akkaraman ewes that are SBVspecific antibody positive without clinical symptoms.

Anahtar Kelime:

Schmallenberg Virüs Seropozitif Akkaraman Koyunlarda Oksidatif Stres

Öz:

Bu çalışmanın amacı Schmallenberg virüs (SBV) seropozitif belirlenen klinik olarak sağlıklı Akkaraman koyunlarda bazı oksidatif stres parametrelerinin (nitrik oksit (NO), malondialdehid (MDA), total antioksidan kapasite (TAK), total oksidan kapasite (TOK), katalaz aktivitesi) değerlendirilmesiyle oksidan-antioksidan durumun belirlenmesidir. Çalışmanın materyalini, ELISA yöntemiyle SBV özgül antikorları yönünden 4 pozitif, 6 şüpheli ve 7 negatif-kontrol kan serumu oluşturdu. Gruplar arasında istatistiksel bir fark olmamasına rağmen (P>0.05) en yüksek NO seviyesi SBV seropozitif serumlarındayken, en düşük TOK seviyesi SBV negatif serumlarda bulundu. Gruplardaki TAS değerleri oldukça benzerdi (P=0.981). SBV seropozitif ve şüpheli serum MDA seviyeleri benzerken SBV seropozitif ve negatif serumların MDA seviyeleri istatistiki olarak farklı bulundu (P=0.021). SBV seropozitif olan serumların katalaz aktiviteleri, SBV şüpheli serumların katalaz aktivitelerinden daha düşük (P=0.030) iken pozitif ve negatif grupların katalaz aktiviteleri ile şüpheli ve negatif grupların katalaz aktiviteleri benzer bulundu. Sonuç olarak; hastalığa ait klinik semptom bulunmayan SBV seropozitif Akkaraman ırkı hayvanlarda MDA seviyesinin etkilendiği belirlendi.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Macun H, ÇINAR M, KÜRŞAT AZKUR A, KALENDER H, ERAT S (2018). Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. , 128 - 134. 10.17094/ataunivbd.315776
Chicago	Macun Hasan Ceyhun,ÇINAR Miyase,KÜRŞAT AZKUR Ahmet,KALENDER Hakan,ERAT Serkan Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. (2018): 128 - 134. 10.17094/ataunivbd.315776
MLA	Macun Hasan Ceyhun,ÇINAR Miyase,KÜRŞAT AZKUR Ahmet,KALENDER Hakan,ERAT Serkan Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. , 2018, ss.128 - 134. 10.17094/ataunivbd.315776
AMA	Macun H,ÇINAR M,KÜRŞAT AZKUR A,KALENDER H,ERAT S Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. . 2018; 128 - 134. 10.17094/ataunivbd.315776
Vancouver	Macun H,ÇINAR M,KÜRŞAT AZKUR A,KALENDER H,ERAT S Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. . 2018; 128 - 134. 10.17094/ataunivbd.315776
IEEE	Macun H,ÇINAR M,KÜRŞAT AZKUR A,KALENDER H,ERAT S "Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus." , ss.128 - 134, 2018. 10.17094/ataunivbd.315776
ISNAD	Macun, Hasan Ceyhun vd. "Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus". (2018), 128-134. https://doi.org/10.17094/ataunivbd.315776

APA	Macun H, ÇINAR M, KÜRŞAT AZKUR A, KALENDER H, ERAT S (2018). Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13(2), 128 - 134. 10.17094/ataunivbd.315776
Chicago	Macun Hasan Ceyhun,ÇINAR Miyase,KÜRŞAT AZKUR Ahmet,KALENDER Hakan,ERAT Serkan Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13, no.2 (2018): 128 - 134. 10.17094/ataunivbd.315776
MLA	Macun Hasan Ceyhun,ÇINAR Miyase,KÜRŞAT AZKUR Ahmet,KALENDER Hakan,ERAT Serkan Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol.13, no.2, 2018, ss.128 - 134. 10.17094/ataunivbd.315776
AMA	Macun H,ÇINAR M,KÜRŞAT AZKUR A,KALENDER H,ERAT S Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018; 13(2): 128 - 134. 10.17094/ataunivbd.315776
Vancouver	Macun H,ÇINAR M,KÜRŞAT AZKUR A,KALENDER H,ERAT S Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018; 13(2): 128 - 134. 10.17094/ataunivbd.315776
IEEE	Macun H,ÇINAR M,KÜRŞAT AZKUR A,KALENDER H,ERAT S "Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus." Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13, ss.128 - 134, 2018. 10.17094/ataunivbd.315776
ISNAD	Macun, Hasan Ceyhun vd. "Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13/2 (2018), 128-134. https://doi.org/10.17094/ataunivbd.315776