The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model

çoban, ganime; göksel, ferdi; GÖLGE, umut hatay; kaymaz, burak; Toman, Hüseyin; KÖMÜRCÜ, Erkam

The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model

Burak KAYMAZ, (Çanakkale Onsekiz Mart Üniversitesi, Ortopedi ve Travmatoloji Anabilim Dalı, Çanakkale, Türkiye)

Ganime ÇOBAN, (Bezmialem Vakıf Üniversitesi, Patoloji Anabilim Dalı, İstanbul, Türkiye)

Ferdi GÖKSEL, (Ereğli Medical Park Hastanesi, Ortopedi ve Travmatoloji Kliniği, Zonguldak, Türkiye)

Hüseyin TOMAN, (Medicana International Hastanesi, Anesteziyoloji ve Reanimasyon Bölümü, İstanbul, Türkiye)

Umut Hatay GÖLGE, (Girilmemişş)

Erkam KÖMÜRCÜ (Medical Park Gaziosmanpaşa Hastanesi, Ortopedi ve Travmatoloji Kliniği, İstanbul, Türkiye)

Aile Hekimliği ve Palyatif Bakım

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Yıl: 2019 Cilt: 4 Sayı: 3 Sayfa Aralığı: 79 - 84 Metin Dili: İngilizce İndeks Tarihi: 02-11-2020

The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model

Öz:

Introduction: Ischemia is reversible or irreversible cell/tissue damage that is secondary to insufficient blood flow to tissues or organs. Ischemia causesmany metabolic and structural changes at the cellular level. Ozone therapy is often used as an antioxidant remedy. This study aimed to investigate theeffects of ozone therapy on extremity ischemia-reperfusion (IR) injury.Methods: Twenty-four New Zealand White (NZW) rabbits were randomly allocated into three groups. Each group consisted of eight rabbits. Group Iwas the control group, Group II was the ischemia group, and Group III, the ozone group. Femoral arteries of the right legs were dissected, and femoralarterial occlusion was performed in Group II and III. Hematological and histopathological evaluation was performed in all groups.Results: The levels of total antioxidant status (TAS) after the surgical procedure was higher in the ozone group compared to the ischemia group(p=0.036). In addition, the levels of malondialdehyde (MDA) after the surgical procedure were lower in the ozone group compared to the ischemiagroup but not statistically significant (p=0.093). The control and ozone groups were histopathologically similar.Conclusions: Ozone therapy may be used as an alternative treatment modality with its anti-inflammatory and antioxidant effects for the treatment ofextremity IR injury

Anahtar Kelime:

Tavşan modelinde ekstremite iskemi-reperfüzyon hasarına ozon tedavisinin etkisi

Öz:

Giriş: İskemi; organı veya dokuyu perfüze eden kan akımındaki yetersizliğe bağlı olarak gelişen geri dönüşümlü veya dönüşümsüz hücre/doku zedelenmesidir. İskemi sonrasında hücrelerde pek çok metabolik ve yapısal değişiklikler oluşmaktadır. Ozon (O3) çeşitli hastalıkları tedavisi için antioksidan olarak terapötik amaçlı kullanılmaktadır. Bu çalışmada, ozon tedavisinin ekstremite iskemi-reperfüzyon hasarı üzerine olan etkisinin incelenmesi amaçlanmıştır. Yöntem: 24 adet New Zealand White (NZW) erişkin erkek tavşan rastgele 3 gruba ayrıldı.Grup1: Kontrol grubu (n:8) Grup II: İskemi grubu (n:8) ve Grup III: Ozon grubu (n:8). Deneklerin sağ bacaklarına femoral arter oklüzyonu uygulanıp Grup II ve Grup III de iskemi-reperfüzyon hasarı uygulandı. Kontrol grubunda disseksiyon yapılıp oklüzyon uygulanmadı. Deneklerin kan değerleri ve sakrifikasyon sonrası kas dokusu histopatolojik değerlendirmesi yapıldı. Bulgular: İşlem sonrası iskemi grubuyla ozon tedavisi uygulanan gruplar karşılaştırıldığında; TAS (total antioksidan status) düzeyinin ozon tedavisi uygulanan grupta anlamlı düzeyde daha yüksek (p=0,036) ve MDA (malondialdehit) düzeyinin de ozon tedavisi uygulanan grupta daha düşük olduğu, ancak istatistiksel olarak anlamlılığa (p=0,093) ulaşmadığı görüldü. Histopatolojik açıdan da ozon grubu ile kontrol grubunun benzer olduğu görüldü. Sonuç: Ozon tedavisi güçlü anti-inflamatuvar ve antioksidan etkinliği ile ekstermite iskemi-reperfüzyon (IR) hasarını azaltmada alternatif bir tedavi seçeneği olabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	kaymaz b, çoban g, göksel f, Toman H, GÖLGE u, KÖMÜRCÜ E (2019). The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. , 79 - 84.
Chicago	kaymaz burak,çoban ganime,göksel ferdi,Toman Hüseyin,GÖLGE umut hatay,KÖMÜRCÜ Erkam The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. (2019): 79 - 84.
MLA	kaymaz burak,çoban ganime,göksel ferdi,Toman Hüseyin,GÖLGE umut hatay,KÖMÜRCÜ Erkam The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. , 2019, ss.79 - 84.
AMA	kaymaz b,çoban g,göksel f,Toman H,GÖLGE u,KÖMÜRCÜ E The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. . 2019; 79 - 84.
Vancouver	kaymaz b,çoban g,göksel f,Toman H,GÖLGE u,KÖMÜRCÜ E The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. . 2019; 79 - 84.
IEEE	kaymaz b,çoban g,göksel f,Toman H,GÖLGE u,KÖMÜRCÜ E "The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model." , ss.79 - 84, 2019.
ISNAD	kaymaz, burak vd. "The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model". (2019), 79-84.

APA	kaymaz b, çoban g, göksel f, Toman H, GÖLGE u, KÖMÜRCÜ E (2019). The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. Aile Hekimliği ve Palyatif Bakım, 4(3), 79 - 84.
Chicago	kaymaz burak,çoban ganime,göksel ferdi,Toman Hüseyin,GÖLGE umut hatay,KÖMÜRCÜ Erkam The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. Aile Hekimliği ve Palyatif Bakım 4, no.3 (2019): 79 - 84.
MLA	kaymaz burak,çoban ganime,göksel ferdi,Toman Hüseyin,GÖLGE umut hatay,KÖMÜRCÜ Erkam The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. Aile Hekimliği ve Palyatif Bakım, vol.4, no.3, 2019, ss.79 - 84.
AMA	kaymaz b,çoban g,göksel f,Toman H,GÖLGE u,KÖMÜRCÜ E The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. Aile Hekimliği ve Palyatif Bakım. 2019; 4(3): 79 - 84.
Vancouver	kaymaz b,çoban g,göksel f,Toman H,GÖLGE u,KÖMÜRCÜ E The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model. Aile Hekimliği ve Palyatif Bakım. 2019; 4(3): 79 - 84.
IEEE	kaymaz b,çoban g,göksel f,Toman H,GÖLGE u,KÖMÜRCÜ E "The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model." Aile Hekimliği ve Palyatif Bakım, 4, ss.79 - 84, 2019.
ISNAD	kaymaz, burak vd. "The effects of ozone therapy on extremity ischemiareperfusion injury in a rabbit model". Aile Hekimliği ve Palyatif Bakım 4/3 (2019), 79-84.