Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods
Yıl: 2020 Cilt: 15 Sayı: 2 Sayfa Aralığı: 130 - 137 Metin Dili: İngilizce DOI: 10.17094/ataunivbd.699247 İndeks Tarihi: 03-11-2020
Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods
Öz: In this study, we aimed to determine acetamipridine’s (ACE) genotoxic effect in mus musculus var. albinos bonemarrow cells by chromosomal aberration (CA) and micronucleus (MN) analysis. Mice were divided into four groups. Group 1determined as negative control (NC), group 2, 3 and 4 were determined as 5 mg/kg ACE, 10 mg/kg ACE, and 15 mg/kg ACEgroups respectively. ACE was given orally for 14 days. CA, mitotic index (MI) and MN frequencies were determined in femoralcells. As a result, no genotoxic effects were observed in groups treated with 5-10 mg/kg ACE compared to negative control.CA rates of 15 mg/kg ACE group was determined to be high (P<0.001). In addition, MI ratio in the 15 mg/kg ACE group waslower than the control group. When the ratios of the polychromatic erythrocyte with micronucleus were examined, nosignificant difference was found between the 5 mg/kg and 10 mg/kg ACE groups and control group. However, a statisticallysignificant increase in MNPCE rates was observed in the 15 mg/kg ACE group compared to control ones (P<0.001). It wasconcluded that ACE at 15 mg/kg dose was genotoxic-cytotoxic.
Anahtar Kelime: Asetamipridin Fare Kemik İliği Hücrelerinde Genotoksik etkisinin KA (Kromozomal Aberasyon) ve MN (Mikronükleus) Test Yöntemleri ile Araştırılması
Öz: Bu çalışma ile asetamipridin (ACE) mus musculus var. albino türü farelerin kemik iliği hücrelerinde kromozomal aberasyon (KA) ve mikronükleus (MN) analizi aracılığı ile genotoksik etkisinin belirlenmesini amaçladık. Fareler dört gruba ayrıldı. 1. grup negatif kontrol grubu olarak belirlendi. 2. grup 5 mg/kg ACE grubu, 3. grup 10 mg/kg ACE grubu ve 4. grup, 15 mg/kg ACE grubu olarak belirlendi ve ACE 14 gün boyunca oral olarak verildi. Femur hücrelerinde kromozomal aberasyon, mitotik indeks (MI) ve MN frekansları belirlendi. Sonuç olarak negatif kontrol grubuyla karşılaştırıldığında 5 mg/kg ve 10 mg/kg ACE uygulanan gruplarda genotoksik etki gözlenmedi. 15 mg/kg ACE uygulanan grupta kromozomal aberasyon oranının yüksek olduğu (P<0.001) belirlendi. Ayrıca 15 mg/kg ACE uygulanan grupta mitotik indeks oranın kontrol grubuna göre düşük olduğu saptandı. MN frekansları incelendiğinde, 5 mg/kg ve 10 mg/kg ACE uygulanan gruplar ile kontrol grubu arasında anlamlı bir fark bulunamadı. Ancak kontrol grubuna göre 15 mg/kg asetamiprid uygulanan grupta, Mikronükleuslu polikromatik eritrosit oranlarında istatistiksel olarak anlamlı bir artış gözlendi (P<0.001). ACE 15 mg/kg dozlarının genotoksik-sitotoksik etkili olduğu sonucuna varıldı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | sandayuk ş, AKSU KILIÇLE P (2020). Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. , 130 - 137. 10.17094/ataunivbd.699247 |
| Chicago | sandayuk şafak,AKSU KILIÇLE PINAR Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. (2020): 130 - 137. 10.17094/ataunivbd.699247 |
| MLA | sandayuk şafak,AKSU KILIÇLE PINAR Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. , 2020, ss.130 - 137. 10.17094/ataunivbd.699247 |
| AMA | sandayuk ş,AKSU KILIÇLE P Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. . 2020; 130 - 137. 10.17094/ataunivbd.699247 |
| Vancouver | sandayuk ş,AKSU KILIÇLE P Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. . 2020; 130 - 137. 10.17094/ataunivbd.699247 |
| IEEE | sandayuk ş,AKSU KILIÇLE P "Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods." , ss.130 - 137, 2020. 10.17094/ataunivbd.699247 |
| ISNAD | sandayuk, şafak - AKSU KILIÇLE, PINAR. "Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods". (2020), 130-137. https://doi.org/10.17094/ataunivbd.699247 |
| APA | sandayuk ş, AKSU KILIÇLE P (2020). Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15(2), 130 - 137. 10.17094/ataunivbd.699247 |
| Chicago | sandayuk şafak,AKSU KILIÇLE PINAR Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 15, no.2 (2020): 130 - 137. 10.17094/ataunivbd.699247 |
| MLA | sandayuk şafak,AKSU KILIÇLE PINAR Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol.15, no.2, 2020, ss.130 - 137. 10.17094/ataunivbd.699247 |
| AMA | sandayuk ş,AKSU KILIÇLE P Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2020; 15(2): 130 - 137. 10.17094/ataunivbd.699247 |
| Vancouver | sandayuk ş,AKSU KILIÇLE P Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2020; 15(2): 130 - 137. 10.17094/ataunivbd.699247 |
| IEEE | sandayuk ş,AKSU KILIÇLE P "Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods." Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15, ss.130 - 137, 2020. 10.17094/ataunivbd.699247 |
| ISNAD | sandayuk, şafak - AKSU KILIÇLE, PINAR. "Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 15/2 (2020), 130-137. https://doi.org/10.17094/ataunivbd.699247 |
