Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?
Yıl: 2020 Cilt: 26 Sayı: 5 Sayfa Aralığı: 657 - 662 Metin Dili: İngilizce DOI: 10.14744/tjtes.2019.79002 İndeks Tarihi: 03-11-2020
Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?
Öz: BACKGROUND: Very high mortality rate in sepsis may be related to oxidative stress. This study was conducted on the rats toinvestigate the presence of oxidative stress and also the potential protective effects of the ß-glucan in the intra-abdominal sepsis modelformed by cecal ligation-perforation (CLP).METHODS: In this study, 30 Male rats were equally divided into three groups as “Sham”, “Sepsis” and “ß-Glucan”. Only laparotomywas performed in the Sham group, and sepsis was induced by CLP in Sepsis and ß-Glucan groups. Following CLP, a single dose of 4mg ß-glucan/kg was also intraperitoneally administered to the β-Glucan group. Blood and tissue (liver, lung and kidney) samples weretaken from Sepsis and ß-Glucan groups after sepsis development determined at the end of the 48th hour, also from the Sham group.The levels of myeloperoxidase (MPO) and advanced oxidation protein products (AOPP) were determined in plasma samples, and themalondialdehyde (MDA) was measured in plasma and tissues.RESULTS: MPO and AOPP were higher in both the Sepsis and ß-Glucan groups; however, plasma and tissue MDA levels were higheronly in the Sepsis group than the Sham group (p<0.05). However, when compared to the Sepsis group, all parameters measured, exceptkidney MDA, were significantly lower in the ß-Glucan group (p<0.05).CONCLUSION: To our knowledge, this is the first study to investigate the AOPP levels in the CLP sepsis model, ROS produced bythe reaction of MPO derived from neutrophils may form oxidative damage to the proteins, compared to the lipids, and ß-glucan maybe used as an alternative agent for sepsis treatment.
Anahtar Kelime: ß-glukan, sıçanlarda deneysel sepsis modelinde oksidatif strese karşı koruyucu rol oynayabilir mi?
Öz: AMAÇ: Sepsiste çok yüksek mortalite oranı oksidatif stres ile ilişkili olabilir. Bu çalışma fareler üzerinde, çekal ligasyon-perforasyon (CLP) ile oluşturulan karıniçi sepsis modelinde oksidatif stresin varlığını ve ayrıca ß-glukanın potansiyel koruyucu etkisini araştırmak için yapılmıştır. GEREÇ VE YÖNTEM: Otuz erkek sıçan eşit olarak “Sham”, “Sepsis” ve “ß-Glukan” olarak üç gruba ayrıldı. Sham grubunda sadece laparotomi yapıldı, Sepsis ve ß-Glukan gruplarında CLP ile sepsis oluşturuldu. ß-Glukan grubuna CLP’yi takiben intraperitonal olarak tek bir doz 4 mg ß-glukan/ kg verildi. Sepsis gelişiminden sonra 48. saatin sonunda Sepsis ve ß-Glukan gruplarından ve Sham grubundan kan ve doku (karaciğer, akciğer ve böbrek) örnekleri alındı. Plazma örneklerinde miyeloperoksidaz (MPO) ve ileri oksidasyon protein ürünleri (AOPP) seviyeleri belirlendi ve plazma ve dokularda malondialdehit (MDA) ölçüldü. BULGULAR: MPO ve AOPP, Sepsis ve ß-Glukan gruplarında daha yüksekti; ancak plazma ve doku MDA düzeyleri sadece Sepsis grubunda Sham grubundan daha yüksekti (p<0.05). Bununla birlikte, Sepsis grubuyla karşılaştırıldığında, böbrek MDA değeri hariç ölçülen tüm parametreler, ß-Glukan grubunda anlamlı olarak daha düşüktü (p<0.05). TARTIŞMA: CLP sepsis modelinde AOPP düzeylerini araştırmak için yapılan bu ilk çalışmada; nötrofillerden salgılanan MPO reaksiyonu ile üretilen ROS, lipitlere kıyasla proteinlerde oksidatif hasar oluşturabilir ve ß-glukan, sepsis tedavisi için alternatif bir ajan olarak kullanılabilir. Anahtar sözcükler: ß-Glukan; ileri oksidasyon protein ürünleri; miyeloperoksidaz; reaktif oksijen türleri; sepsis.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Demir M, KÖSE K, YAZICI C, Sözüer E (2020). Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. , 657 - 662. 10.14744/tjtes.2019.79002 |
| Chicago | Demir Meltem,KÖSE KADER,YAZICI Cevat,Sözüer Erdoğan Mütevelli Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. (2020): 657 - 662. 10.14744/tjtes.2019.79002 |
| MLA | Demir Meltem,KÖSE KADER,YAZICI Cevat,Sözüer Erdoğan Mütevelli Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. , 2020, ss.657 - 662. 10.14744/tjtes.2019.79002 |
| AMA | Demir M,KÖSE K,YAZICI C,Sözüer E Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. . 2020; 657 - 662. 10.14744/tjtes.2019.79002 |
| Vancouver | Demir M,KÖSE K,YAZICI C,Sözüer E Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. . 2020; 657 - 662. 10.14744/tjtes.2019.79002 |
| IEEE | Demir M,KÖSE K,YAZICI C,Sözüer E "Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?." , ss.657 - 662, 2020. 10.14744/tjtes.2019.79002 |
| ISNAD | Demir, Meltem vd. "Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?". (2020), 657-662. https://doi.org/10.14744/tjtes.2019.79002 |
| APA | Demir M, KÖSE K, YAZICI C, Sözüer E (2020). Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. Ulusal Travma ve Acil Cerrahi Dergisi, 26(5), 657 - 662. 10.14744/tjtes.2019.79002 |
| Chicago | Demir Meltem,KÖSE KADER,YAZICI Cevat,Sözüer Erdoğan Mütevelli Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. Ulusal Travma ve Acil Cerrahi Dergisi 26, no.5 (2020): 657 - 662. 10.14744/tjtes.2019.79002 |
| MLA | Demir Meltem,KÖSE KADER,YAZICI Cevat,Sözüer Erdoğan Mütevelli Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. Ulusal Travma ve Acil Cerrahi Dergisi, vol.26, no.5, 2020, ss.657 - 662. 10.14744/tjtes.2019.79002 |
| AMA | Demir M,KÖSE K,YAZICI C,Sözüer E Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(5): 657 - 662. 10.14744/tjtes.2019.79002 |
| Vancouver | Demir M,KÖSE K,YAZICI C,Sözüer E Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(5): 657 - 662. 10.14744/tjtes.2019.79002 |
| IEEE | Demir M,KÖSE K,YAZICI C,Sözüer E "Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?." Ulusal Travma ve Acil Cerrahi Dergisi, 26, ss.657 - 662, 2020. 10.14744/tjtes.2019.79002 |
| ISNAD | Demir, Meltem vd. "Does ß-glucan can have a protective role against oxidative stress in the experimental sepsis model in rats?". Ulusal Travma ve Acil Cerrahi Dergisi 26/5 (2020), 657-662. https://doi.org/10.14744/tjtes.2019.79002 |
