Yıl: 2020 Cilt: 26 Sayı: 5 Sayfa Aralığı: 663 - 670 Metin Dili: İngilizce DOI: 10.14744/tjtes.2019.45570 İndeks Tarihi: 03-11-2020

The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis

Öz:
BACKGROUND: Curcumin is an antioxidant and anti-inflammatory molecule known to be a potent inhibitor of nuclear factor kappaB (NF-kappa B). In this study, we aimed to investigate the therapeutic effects of curcumin on colitis induced by a 2,4,6-trinitrobenzenesulfonic acid (TNBS).METHODS: After the induction of colitis under anesthesia, 42 rats were divided into six groups as follows; the curcumin oral group,curcumin (20 mg/kg); the corn oil oral group, corn oil (20 mg/kg) using gastric gavage, the curcumin rectal group, curcumin; the cornoil rectal group, corn oil; the control group, 1 mL saline solution (0.9% NaCl) were administered using the rectal route. In the shamgroup, only rectal catheterization was performed. At the end of the seven day, the blood and intestinal tissue samples were obtainedfor histopathological examination and for MPO, MDA, NO, PDGF, IL-6, TNF-alpha, NF-kappaB.RESULTS: The macroscopic damage score was significantly higher in curcumin oral, corn oil oral and saline groups when compared tothe sham group (p<0.05). The significant differences between groups were evaluated using the biochemical analysis of intestinal tissuefor IL-6, NO, NF-κB, PDGF, TNF-α, MDA, MPO (p<0.05). NF-κB levels of blood in curcumin oral, curcumin rectal, sham, corn oiloral, corn oil rectal groups were significantly increased when compared to saline rectal group (p≤0.001). NF-κB serum levels of cornoil rectal and control group (p≤0.001) were lower than the sham group (p=0.012).CONCLUSION: The effects of curcumin improved possibly by modulating the NF-κB signaling pathway should be considered againstcolitis alone or in combination with the conventional anti-colitic therapies in future studies.
Anahtar Kelime:

Kurkumin’in PDGF ekspresyonu ve NF-kappa B üzerindeki etkisi: TNBS kolit modeli

Öz:
AMAÇ: Kurkumin, nükleer faktör kappa B’nin (NF-kappa B) güçlü bir inhibitörü olduğu bilinen antioksidan ve antienflamatuvar bir moleküldür. Kurkumin’in 2,4,6-trinitrobenzen sülfonik asit (TNBS) ile indüklenen kolit modeli üzerindeki etkisini araştırmayı amaçladık. GEREÇ VE YÖNTEM: Anestezi altında kolit indüksiyonundan sonra, 42 sıçan altı gruba ayrıldı; kurkumin oral grup, kurkumin (20 mg/kg); mısır yağı oral grubu, mısır yağı (20 mg/kg) mide gavajı yoluyla, kurkumin rektal grubu, kurkumin; mısır yağı rektal grubu, mısır yağı. Kontrol grubuna 1 mL salin solüsyonu (%0.9 NaCl) rektal yolla uygulandı. Sham grubunda sadece rektal kateterizasyon yapıldı. Yedi gün sonunda, histopatolojik inceleme ve MPO, MDA, NO, PDGF, IL-6, TNF-alfa, NF-kappa B için kan ve bağırsak doku örnekleri alındı. BULGULAR: Makroskobik hasar skoru, kurkumin oral, mısır yağı oral ve salin gruplarında sham grubuna göre anlamlı olarak yüksek bulundu (p<0.05). Gruplar arasındaki anlamlı fark, IL-6, NO, NF-kB, PDGF, TNF-α, MDA, MPO intestinal dokunun biyokimyasal analizinde değerlendirildi (p<0.05). Kurkumin oral, kurkumin rektal, sham, mısır yağı oral, mısır yağı rektal gruplarında NF-kB kan seviyeleri, salin rektal grubuna göre anlamlı olarak arttı (p=0.001). Mısır yağı rektal ve kontrol grubunun NF-kB serum seviyeleri (p≤0.001) sham grubundan düşük bulundu (p=0.012). TARTIŞMA: Kurkumin’in, NF-kB sinyal yolundaki duzenleyici etkisi muhtemelen iyileştirici özelliği ile gelecekteki çalışmalarda tek başına kolite karşı veya konvansiyonel anti-kolitik tedaviler de kombinasyon halinde düşünülmelidir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Altinel Y, Yalcin S, Ercan G, Yavuz E, ercetin c, Gülcicek O, Çelik A, Ozkaya G, Uzun H (2020). The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. , 663 - 670. 10.14744/tjtes.2019.45570
Chicago Altinel Yuksel,Yalcin Senay,Ercan Gulcin,Yavuz Erkan,ercetin candas,Gülcicek Osman Bilgin,Çelik Atilla,Ozkaya Guven,Uzun Hafize The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. (2020): 663 - 670. 10.14744/tjtes.2019.45570
MLA Altinel Yuksel,Yalcin Senay,Ercan Gulcin,Yavuz Erkan,ercetin candas,Gülcicek Osman Bilgin,Çelik Atilla,Ozkaya Guven,Uzun Hafize The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. , 2020, ss.663 - 670. 10.14744/tjtes.2019.45570
AMA Altinel Y,Yalcin S,Ercan G,Yavuz E,ercetin c,Gülcicek O,Çelik A,Ozkaya G,Uzun H The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. . 2020; 663 - 670. 10.14744/tjtes.2019.45570
Vancouver Altinel Y,Yalcin S,Ercan G,Yavuz E,ercetin c,Gülcicek O,Çelik A,Ozkaya G,Uzun H The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. . 2020; 663 - 670. 10.14744/tjtes.2019.45570
IEEE Altinel Y,Yalcin S,Ercan G,Yavuz E,ercetin c,Gülcicek O,Çelik A,Ozkaya G,Uzun H "The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis." , ss.663 - 670, 2020. 10.14744/tjtes.2019.45570
ISNAD Altinel, Yuksel vd. "The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis". (2020), 663-670. https://doi.org/10.14744/tjtes.2019.45570
APA Altinel Y, Yalcin S, Ercan G, Yavuz E, ercetin c, Gülcicek O, Çelik A, Ozkaya G, Uzun H (2020). The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. Ulusal Travma ve Acil Cerrahi Dergisi, 26(5), 663 - 670. 10.14744/tjtes.2019.45570
Chicago Altinel Yuksel,Yalcin Senay,Ercan Gulcin,Yavuz Erkan,ercetin candas,Gülcicek Osman Bilgin,Çelik Atilla,Ozkaya Guven,Uzun Hafize The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. Ulusal Travma ve Acil Cerrahi Dergisi 26, no.5 (2020): 663 - 670. 10.14744/tjtes.2019.45570
MLA Altinel Yuksel,Yalcin Senay,Ercan Gulcin,Yavuz Erkan,ercetin candas,Gülcicek Osman Bilgin,Çelik Atilla,Ozkaya Guven,Uzun Hafize The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. Ulusal Travma ve Acil Cerrahi Dergisi, vol.26, no.5, 2020, ss.663 - 670. 10.14744/tjtes.2019.45570
AMA Altinel Y,Yalcin S,Ercan G,Yavuz E,ercetin c,Gülcicek O,Çelik A,Ozkaya G,Uzun H The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(5): 663 - 670. 10.14744/tjtes.2019.45570
Vancouver Altinel Y,Yalcin S,Ercan G,Yavuz E,ercetin c,Gülcicek O,Çelik A,Ozkaya G,Uzun H The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(5): 663 - 670. 10.14744/tjtes.2019.45570
IEEE Altinel Y,Yalcin S,Ercan G,Yavuz E,ercetin c,Gülcicek O,Çelik A,Ozkaya G,Uzun H "The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis." Ulusal Travma ve Acil Cerrahi Dergisi, 26, ss.663 - 670, 2020. 10.14744/tjtes.2019.45570
ISNAD Altinel, Yuksel vd. "The efficacy of curcumin on PDGF expression and NF-kappa B pathway: TNBS-induced colitis". Ulusal Travma ve Acil Cerrahi Dergisi 26/5 (2020), 663-670. https://doi.org/10.14744/tjtes.2019.45570