Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants
Yıl: 2020 Cilt: 15 Sayı: 1 Sayfa Aralığı: 43 - 50 Metin Dili: İngilizce DOI: 10.17094/ataunivbd.680945 İndeks Tarihi: 03-11-2020
Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants
Öz: In this study, the protective effect of silymarin was investigated with histopathological methods in terms of cancerpatterns and cytological variants in the hepatocellular carcinoma model created by applying diethylnitrosamineexperimentally in rats. For this purpose, the rats were randomly divided into 5 groups. The rats in the control group were fedpellet feed and drinking water for 20 weeks. Animals in Sham group were administered with propylene glycol dissolved in0.9% NaCl intraperitoneally 3 times a week for 20 weeks. Animals in DEN group administered DEN at a dose of 50 mg/kg oncea week for 20 weeks. The rats in the DEN +Silymarin group were Silymarin intraperitoneally administered for 21 weeks 3 timesa week at a dose of 100 mg/kg, starting 1 week prior to the administration of DEN. Silymarin was administered to rats in thegroup of Silymarin 3 times a week intraperitoneally for 20 weeks at a dose of 100 mg/kg. At the end of the study, systemicnecropsy of the animals was performed and liver tissue samples were taken. After routine tissue procedures, sections wereexamined under the light microscope. We observed that Hepatocellular carcinoma occurred in DEN and DEN + Silymaringroups.
Anahtar Kelime: Wistar Ratlarında Dietilnitrozamin Uygulanarak Oluşturulan Hepatoselüler Karsinom Modelinde Silimarin’in Koruyucu Etkisi; Kanser Paternleri ve Sitolojik Varyantlar
Öz: Bu çalışmada, ratlarda deneysel olarak Dietilnitrozamin uygulanarak oluşturulan Hepatoselüler karsinom modelinde Silimarin’in koruyucu etkisi kanser paternleri ve sitolojik varyantlar açısından histopatolojik metodlarla araştırılmıştır. Bu amaçla ratlar 5 gruba ayrıldı. Kontrol grubundaki ratlara 20 hafta boyunca pelet yem ve içme suyu verildi. Sham grubundaki hayvanlara %0.9 NaCl içerisinde çözdürülmüş propilen glikol intraperitoneal yolla haftada 3 kez olmak üzere 20 hafta boyunca verildi. DEN grubundaki hayvanlara 50 mg/kg dozunda haftada 1 kez olmak üzere 20 hafta boyunca DEN uygulaması yapıldı. DEN+Silimarin grubundaki ratlara DEN uygulamasından 1 hafta önce başlayacak şekilde 100 mg/kg dozunda haftada 3 kez olmak üzere 21 hafta boyunca intraperitoneal yolla Silimarin uygulandı. Silimarin grubundaki ratlara 100 mg/kg dozunda 20 hafta boyunca intraperitoneal yolla haftada 3 kez olmak üzere Silimarin uygulandı. Çalışmanın sonunda, hayvanların sistemik nekropsisi yapıldı ve karaciğer doku örnekleri alındı. Rutin doku takip işlemlerinden sonra kesitler ışık mikroskobu altında değerlendirildi. DEN ve DEN+Silimarin gruplarında Hepatoselüler karsinom oluştuğunu gözlemledik.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Karakurt E, DAĞ S (2020). Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. , 43 - 50. 10.17094/ataunivbd.680945 |
| Chicago | Karakurt Emin,DAĞ SERPİL Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. (2020): 43 - 50. 10.17094/ataunivbd.680945 |
| MLA | Karakurt Emin,DAĞ SERPİL Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. , 2020, ss.43 - 50. 10.17094/ataunivbd.680945 |
| AMA | Karakurt E,DAĞ S Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. . 2020; 43 - 50. 10.17094/ataunivbd.680945 |
| Vancouver | Karakurt E,DAĞ S Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. . 2020; 43 - 50. 10.17094/ataunivbd.680945 |
| IEEE | Karakurt E,DAĞ S "Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants." , ss.43 - 50, 2020. 10.17094/ataunivbd.680945 |
| ISNAD | Karakurt, Emin - DAĞ, SERPİL. "Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants". (2020), 43-50. https://doi.org/10.17094/ataunivbd.680945 |
| APA | Karakurt E, DAĞ S (2020). Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15(1), 43 - 50. 10.17094/ataunivbd.680945 |
| Chicago | Karakurt Emin,DAĞ SERPİL Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 15, no.1 (2020): 43 - 50. 10.17094/ataunivbd.680945 |
| MLA | Karakurt Emin,DAĞ SERPİL Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol.15, no.1, 2020, ss.43 - 50. 10.17094/ataunivbd.680945 |
| AMA | Karakurt E,DAĞ S Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2020; 15(1): 43 - 50. 10.17094/ataunivbd.680945 |
| Vancouver | Karakurt E,DAĞ S Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2020; 15(1): 43 - 50. 10.17094/ataunivbd.680945 |
| IEEE | Karakurt E,DAĞ S "Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants." Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15, ss.43 - 50, 2020. 10.17094/ataunivbd.680945 |
| ISNAD | Karakurt, Emin - DAĞ, SERPİL. "Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 15/1 (2020), 43-50. https://doi.org/10.17094/ataunivbd.680945 |
