The aim of the present study was to evaluate the effects of exogenous oxytocin and PGF2α on seminal quality and libido sexualis in bucks.To investigate the role of these hormones on male fertility, semen samples from 20 Norduz bucks (3-4 years of age) were collected withan artificial vagina twice a week with five replications in breeding season. Bucks were randomly assigned to five groups, control groupwas administered with 2 mL of sodium chloride, 0.9% (w/v)) i.m., whilst the experimental groups were administered with oxytocin 10 IU,i.v. (Group 1, n = 5), oxytocin 20 IU, i.v. (Group 2, n = 5), PGF2α 5 mg, i.m. (Group 3, n = 5) or PGF2α 10 mg, i.m. (Group 4, n = 5) 20 min beforeeach collection. There was no statistical difference between the treatment groups in terms of motility (P>0.05). However, semen volume,concentration, abnormal spermatozoa rate, intact membrane rate and libido results were statistically significant among the different groups(P<0.05). As a result, administration of 20 IU oxytocin twenty minutes prior to semen collection in bucks did not improve overall semenquality, however, libido, semen volume, and sperm concentrations were increased. In contrast to oxytocin, PGF2α administration has led to aslight decrease in libido and has shown moderate effects on semen quality.