Yıl: 2020 Cilt: 36 Sayı: 2 Sayfa Aralığı: 107 - 114 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2020.267 İndeks Tarihi: 04-11-2020

Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers

Öz:
Aim: It is aimed to determine the preventive effect of black seed (Ni-gella sativa L; NS) and its pharmaceutically active substance volatilefatty acid thymoquinone on relative liver weight increase in broilerin aflatoxicosis..Materials and Methods: One hundred twenty 1-d-old broiler chickswere used and divided into 6 equal groups and fed for 28 days. Ani-mals were received feed with 2 mg/kg total aflatoxin (AF; AFB1,AFB2, AFG1 and AFG2), 5% black seed (NS) and 300 mg/kg thymo-quinone (TQ), according to the groups; Control, AF, NS, TQ, AF+NSand AF+TQ. At the end of the experiment, euthanasia was performedby decapitation after determination of the live weight of ten broi-ler chickens in all groups, and systemic necropsies were performed.The liver, the main target organ for AF, was removed and weighedto determine relative organ weight. The relative liver weights werecalculated as % (liver weight x 100 / live body weight).Results: Aflatotoxin application significantly increased the relativeweight of the liver (P<0,01). While the difference was not significantin the TQ group compared to the control group, a significant increasein liver weight was found in the NS group. Addition of TQ to feedswith AF significantly reduced the relative liver weight values to nor-mal. NS effect was found more partial compared to TQ.Conclusion: In conclusion, it was thought that TQ and NS preventedthe relative liver weight increase, an important biomarker for afla-toxicosis, by probably ameliorating lipid metabolism and transportwhich is impaired by the toxic effect of AF
Anahtar Kelime:

Çörek otu tohumu ve timokinonun broylerlerde aflatoksinin neden olduğu relatif karaciğer ağırlığı artışı üzerine koruyucu etkisi

Öz:
Amaç: Bu çalışmada çörek otu tohumu ve bunun farmasötik olarak etkin maddesi timokinonun, broylerlerde aflatoksikozisde şekille- nen relatif karaciğer ağırlık artışını önleyici etkinliklerinin belirlen- mesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada 120 adet 1 günlük broyler civciv kul- lanıldı. Bu civcivler 6 eşit gruba bölünerek 28 gün beslendi. Hayvan- lara, gruplarına göre (Kontrol, AF, NS, TQ, AF+NS ve AF+TQ) 2 mg/ kg total aflatoksin (AF; AFB1, AFB2, AFG1 ve AFG2), % 5 çörek otu tohumu (NS) ve 300 mg/kg timokinon (TQ) içeren yemler verildi. Deneme süresi sonunda gruplardaki 10’ar pilicin canlı ağırlıkları belirlendikten sonra dekapitasyon ile ötenazi uygulanarak sistemik nekropsileri yapıldı. Aflatoksin için hedef organ kabul edilen karaci- ğer uzaklaştırılarak relatiforgan ağırlığının belirlenmesi için tartıldı. Karaciğerin relatif organ ağırlığı % olarak (karaciğer ağırlığı x 100 / canlı vücut ağırlığı) hesaplandı. Bulgular: Aflatoksin uygulamasının relatif karaciğer ağırlığını önemli oranda artırdığı belirlendi (P<0,01). Timokinon grubu pi- liçlerin relatif karaciğer ağrılığı ile sağlıklı kontrol grubu arasındaki fark önemsizken, NS grubunda önemli derecede artış bulundu. Afla- toksinli diyete TQ ilavesinin relatif karaciğer ağırlığını önemli oran- da normal değerlere düşürdüğü saptandı.Çörek otu tohumunun etkisinin ise TQ’a kıyasla daha kısmi olduğu gözlendi. Öneri: Sonuç olarak, TQ ve NS'nin, AF'nin toksik etkisinden dolayı bozulmuş lipit metabolizmasını ve taşınmasını düzelterek, aflatok- sikozisin önemli belirteçlerinden biri olan relatif karaciğer ağırlığı artışını önlediği değerlendirilmiştir
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ATEŞ M, ORTATATLI M (2020). Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. , 107 - 114. 10.15312/EurasianJVetSci.2020.267
Chicago ATEŞ Mehmet Burak,ORTATATLI MUSTAFA Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. (2020): 107 - 114. 10.15312/EurasianJVetSci.2020.267
MLA ATEŞ Mehmet Burak,ORTATATLI MUSTAFA Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. , 2020, ss.107 - 114. 10.15312/EurasianJVetSci.2020.267
AMA ATEŞ M,ORTATATLI M Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. . 2020; 107 - 114. 10.15312/EurasianJVetSci.2020.267
Vancouver ATEŞ M,ORTATATLI M Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. . 2020; 107 - 114. 10.15312/EurasianJVetSci.2020.267
IEEE ATEŞ M,ORTATATLI M "Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers." , ss.107 - 114, 2020. 10.15312/EurasianJVetSci.2020.267
ISNAD ATEŞ, Mehmet Burak - ORTATATLI, MUSTAFA. "Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers". (2020), 107-114. https://doi.org/10.15312/EurasianJVetSci.2020.267
APA ATEŞ M, ORTATATLI M (2020). Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. Eurasian Journal of Veterinary Sciences, 36(2), 107 - 114. 10.15312/EurasianJVetSci.2020.267
Chicago ATEŞ Mehmet Burak,ORTATATLI MUSTAFA Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. Eurasian Journal of Veterinary Sciences 36, no.2 (2020): 107 - 114. 10.15312/EurasianJVetSci.2020.267
MLA ATEŞ Mehmet Burak,ORTATATLI MUSTAFA Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. Eurasian Journal of Veterinary Sciences, vol.36, no.2, 2020, ss.107 - 114. 10.15312/EurasianJVetSci.2020.267
AMA ATEŞ M,ORTATATLI M Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. Eurasian Journal of Veterinary Sciences. 2020; 36(2): 107 - 114. 10.15312/EurasianJVetSci.2020.267
Vancouver ATEŞ M,ORTATATLI M Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers. Eurasian Journal of Veterinary Sciences. 2020; 36(2): 107 - 114. 10.15312/EurasianJVetSci.2020.267
IEEE ATEŞ M,ORTATATLI M "Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers." Eurasian Journal of Veterinary Sciences, 36, ss.107 - 114, 2020. 10.15312/EurasianJVetSci.2020.267
ISNAD ATEŞ, Mehmet Burak - ORTATATLI, MUSTAFA. "Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers". Eurasian Journal of Veterinary Sciences 36/2 (2020), 107-114. https://doi.org/10.15312/EurasianJVetSci.2020.267