Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis
Yıl: 2020 Cilt: 36 Sayı: 2 Sayfa Aralığı: 127 - 139 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2020.270 İndeks Tarihi: 04-11-2020
Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis
Öz: Aim: Mastitis is one of the most common diseases of dairy cattle andcauses significant economic losses. Staphylococcus aureus producesmany virulence factors that facilitate the adhesion and penetrationof damaged tissues, and thereby, cause subclinical mastitis. Thisstudy was aimed at investigating the phenotypic and genotypic cha-racteristics and antimicrobial susceptibility of S. aureus..Materials and Methods: A total of 241 S. aureus strains isolatedfrom bovine mastitis cases were tested phenotypically (catalase,coagulase, haemolysis, DNase, mannitol fermentation and biofilmformation) and genotypically (by the polymerase chain reaction(PCR) technique). Antimicrobial susceptibility was tested using 15different antibiotics.Results: While the isolates showed different levels of haemolytic ac-tivity (β 47%, α 42%, γ 10% and δ 1%), only the β-haemolytic strainsproduced a positive CAMP-like reaction. Although all isolates wereable to grow on MSA containing 7.5% NaCl, mannitol fermentationactivity was observed in 80.5% of the isolates. The nuc gene was de-tected in all isolates, but only 84.2% of the isolates showed DNaseactivity. The Congo red agar method can be used to detect the biofilmforming capability of isolates, but the crystal violet staining methodgives more reliable results. The sec gene was the most common en-terotoxin genes (84%). Three isolates harboured the mecA gene, butwere sensitive to methicillin.Conclusion: Phenotypic variations among isolates result in themisclassification of S. aureus strains and require the use ofmolecularmethods. The rapid and accurate molecular typing of S. aureus canaid in both determining the prevalence of this infectious microorga-nism and preventing epidemic infections.
Anahtar Kelime: Mastitisli süt ineklerinden izole edilen Staphylococcus aureus’ların fenotipik ve genotipik karakterizasyonu ve antimikrobiyal direnç genlerinin belirlenmesi
Öz: Amaç: Mastitis, önemli ekonomik kayıplara neden olan süt inekleri- nin en yaygın hastalıklarından biridir. Staphylococcus aureus, subk- linik mastitise neden olan penetrasyonunu ve dokuların tahribatını kolaylaştıran çeşitli virülans faktörlerine sahiptir. Bu çalışmada, S. aureus'un fenotipik ve genotipik özelliklerini ve antimikrobiyal du- yarlılığını araştırılması amaçlanmıştır. Gereç ve Yöntem: Mastitisli süt ineklerinden izole edilen toplam 241 S. aureus suşu fenotipik olarak (katalaz, koagülaz, hemoliz, DNaz, mannitol fermantasyonu ve biyofilm oluşturma) ve genotipik olarak polimeraz zincir reaksiyon tekniği (PCR) kullanılarak test edildi. An- timikrobiyal duyarlılık testi 15 farklı antibiyotik kullanılarak yapıldı. Bulgular: İzolatlar farklı hemoliz aktivitesi göstermiştir (β %47, α %42, γ %10 ve δ %1), ancak sadece β-hemolitik suşlar CAMP reak- siyonu göstermiştir. Tüm izolatlar %7,5 NaCl içeren MSA üzerinde büyüyebilmesine rağmen, izolatların %80,5'inde mannitol ferman- tasyon aktivitesi gözlenmiştir. Nuc geni tüm izolatlarda tespit edil- di, ancak izolatların sadece %84,2'si DNase aktivitesi göstermiştir. Kongo kırmızı agar biyofilmleri tespit etmek için bir tarama yöntemi olarak kullanılabilirken, kristal viyole boyama yöntemi daha yeterli sonuçlar verir. Sec geni, enterotoksin genleri arasında en sık rastla- nan gendir (%84). MecA genini barındırdığı üç izolat tespit edildi, ancak metisiline duyarlıydı. Öneri: Sonuç olarak, izolatların fenotipik özelliklerindeki değişim S. aureus izolatlatının yanlış sınıflandırılmasına yol açmakta ve mole- küler yöntemlerin kullanılması ihtiyacını arttırmaktadır. S. aureus'un hızlı ve doğru moleküler tiplenmesi, bu bulaşıcı mikroorganizmanın prevalansını belirleyebilir ve salgın enfeksiyonları önleyebilir.m kul- lanımının oksidatif strese karşı koruyucu etkisi görülmüştür.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | M. Saeed M, HADIMLI H (2020). Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. , 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| Chicago | M. Saeed Mustafa,HADIMLI HASAN HÜSEYIN Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. (2020): 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| MLA | M. Saeed Mustafa,HADIMLI HASAN HÜSEYIN Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. , 2020, ss.127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| AMA | M. Saeed M,HADIMLI H Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. . 2020; 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| Vancouver | M. Saeed M,HADIMLI H Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. . 2020; 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| IEEE | M. Saeed M,HADIMLI H "Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis." , ss.127 - 139, 2020. 10.15312/EurasianJVetSci.2020.270 |
| ISNAD | M. Saeed, Mustafa - HADIMLI, HASAN HÜSEYIN. "Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis". (2020), 127-139. https://doi.org/10.15312/EurasianJVetSci.2020.270 |
| APA | M. Saeed M, HADIMLI H (2020). Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. Eurasian Journal of Veterinary Sciences, 36(2), 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| Chicago | M. Saeed Mustafa,HADIMLI HASAN HÜSEYIN Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. Eurasian Journal of Veterinary Sciences 36, no.2 (2020): 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| MLA | M. Saeed Mustafa,HADIMLI HASAN HÜSEYIN Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. Eurasian Journal of Veterinary Sciences, vol.36, no.2, 2020, ss.127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| AMA | M. Saeed M,HADIMLI H Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. Eurasian Journal of Veterinary Sciences. 2020; 36(2): 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| Vancouver | M. Saeed M,HADIMLI H Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis. Eurasian Journal of Veterinary Sciences. 2020; 36(2): 127 - 139. 10.15312/EurasianJVetSci.2020.270 |
| IEEE | M. Saeed M,HADIMLI H "Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis." Eurasian Journal of Veterinary Sciences, 36, ss.127 - 139, 2020. 10.15312/EurasianJVetSci.2020.270 |
| ISNAD | M. Saeed, Mustafa - HADIMLI, HASAN HÜSEYIN. "Determination of phenotypical and genotypical characterization and antimicrobial re - sistance genes of Staphylococcus aureus isolated from milk of dairy cows with mastitis". Eurasian Journal of Veterinary Sciences 36/2 (2020), 127-139. https://doi.org/10.15312/EurasianJVetSci.2020.270 |
