Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR
Yıl: 2020 Cilt: 31 Sayı: 1 Sayfa Aralığı: 47 - 51 Metin Dili: İngilizce İndeks Tarihi: 07-11-2020
Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR
Öz: Staphylococcus aureus (S.aureus) can cause serious fatal infections due to several enzymes and toxins that it secretes when it encounters favorable conditions.Thus, S.aureus is one of the most important pathogens currently causing hospital and community infections. Methicillin-resistant S.aureus (MRSA) strains are resistant to many antibiotics and have the ability to rapidly develop resistance to currently used antibiotics during treatment. MRSA origins cause serious infections with high mortality and also increase the rate of hospital infections that require difficult and longer hospitalization and the use of greater quantities of antibiotics in risk-prone units in hospitals. Another factor that is as important as MRSA in S.aureus pathogenicity is the Panton-Valentine leucocidin (PVL) toxin secreted by S.aureus strains, most commonly isolated from deadly necrotizing pneumonias and community-acquired soft skin tissue infections. PVL is especially observed in community-acquired S.aureus strains. However, studies showed that PVL-positive origins began to spread rapidly to hospital environments. In this study, methicillin-sensitive S.aureus, methicillin-resistant coagulase-negative Staphylococcus (MR-CNS), and Panton-Valentine leukocidine genes were identified by real-time polymerase chain reaction (RT-PCR) by investigating the rate of nasal S.aureus carriers (NSAC) in nasal swab samples collected from hospital staff belonging to the highest risk group for staphylococcal infections, at which screening is aimed. Classical NSAC scans were performed on nasal swap samples collected from 98 healthcare professionals in Hospital. Nasal S.aureus (4 MSSA,13 MRSA) was detected in 17 samples(17.35%). During detection, 48 MR-CNS were detected. In addition, 61 mecA (+),17 nuc (+),1 pvl (+) genes were detected in RT-PCR samples directly from the nasal swabs collected in accordance with the culture method.
Anahtar Kelime: Hastane çalışanlarında Nazal Staphylococcus aureus taşıyıcılığı ve PVL ile mecA genlerinin RT-PCR ile hızlı tespiti
Öz: Staphylococcus aureus (S. aureus) kommensal bir bakteri olarak hem insanla beraber yaşamakta, hem de fırsatçı olarak uygun koşulları bulduğunda salgıladığı bazı enzim ve toksinler nedeniyle çok ciddi ölümcül enfeksiyonlara neden olabilmektedir. Bu nedenle günümüzde S. aureus hastane ve toplum kaynaklı enfeksiyonlara neden olan en önemli patojenlerden biridir. Metisilin dirençli S. aureus (MRSA) kökenleri birçok antibiyotiğe karşı dirençlidirler ve tedavi süresince kullanılan günümüz antibiyotiklerine karşı çok hızlı bir şeklide direnç geliştirme kabiliyetine sahiptirler. Bundan dolayıdır ki MRSA enfeksiyonları normal S. aureus enfeksiyonlarına oranla daha şiddetli ve inatçı enfeksiyonlara neden olmaktadırlar. S. aureus patojenitesinde MRSA kadar önemli olan diğer bir etken, özellikle ölümcül nekrotizan pnömonilerden ve toplum kökenli deri, yumuşak doku enfeksiyonlarından izole edilen S.aureus suşları tarafından salgılanan Panton-Valentine lökosidin (PVL) toksinidir. PVL özellikle toplum kökenli S.aureus suşlarında gözlenmektedir. Ancak yapılan çalışmalar PVL pozitif kökenlilerin hastane ortamlarına hızla yayılmaya başladığını göstermiştir. Bu çalışmada stafilokok enfeksiyonları için en yüksek risk grubunda yer alan hastane personelinden alınan nazal swap örneklerinde nazal S.aureus taşıyıcılığı (NSAT) oranının araştırılması ile rt-PCR ile Metisiline Duyarlı S. aureus, Metisiline Dirençli Koagülaz Negatif Stafilokok ve Panton-Valentine lökosidin virülans genlerin taramasının yapılması amaçlanmıştır. Devlet hastanesinde çalışan personelden alınan nazal swap örneklerinden klasik NSAT taraması yapılmış 98 sağlık personelinin 17’sinde (%17,35) nazal S.aureus taşıyıcılığı (4’ü MSSA, 13’ü MRSA ) saptanırken, 48’inde MR-KNS tespit edilmiştir. Ayrıca alınan nazal swablardan direk olarak yapılan RT-PCR örneklerinde kültür metodu ile birebir uyumlu olarak 61 mecA(+), 17 nuc(+), 1 pvl(+) genleri tespit edilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA | Dülger D, Ekici S, ALBUZ O, Pakdemirli A (2020). Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. , 47 - 51. |
Chicago | Dülger Dilek,Ekici Seda,ALBUZ OZGUR,Pakdemirli Ahu Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. (2020): 47 - 51. |
MLA | Dülger Dilek,Ekici Seda,ALBUZ OZGUR,Pakdemirli Ahu Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. , 2020, ss.47 - 51. |
AMA | Dülger D,Ekici S,ALBUZ O,Pakdemirli A Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. . 2020; 47 - 51. |
Vancouver | Dülger D,Ekici S,ALBUZ O,Pakdemirli A Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. . 2020; 47 - 51. |
IEEE | Dülger D,Ekici S,ALBUZ O,Pakdemirli A "Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR." , ss.47 - 51, 2020. |
ISNAD | Dülger, Dilek vd. "Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR". (2020), 47-51. |
APA | Dülger D, Ekici S, ALBUZ O, Pakdemirli A (2020). Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. Etlik Veteriner Mikrobiyoloji Dergisi, 31(1), 47 - 51. |
Chicago | Dülger Dilek,Ekici Seda,ALBUZ OZGUR,Pakdemirli Ahu Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. Etlik Veteriner Mikrobiyoloji Dergisi 31, no.1 (2020): 47 - 51. |
MLA | Dülger Dilek,Ekici Seda,ALBUZ OZGUR,Pakdemirli Ahu Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. Etlik Veteriner Mikrobiyoloji Dergisi, vol.31, no.1, 2020, ss.47 - 51. |
AMA | Dülger D,Ekici S,ALBUZ O,Pakdemirli A Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. Etlik Veteriner Mikrobiyoloji Dergisi. 2020; 31(1): 47 - 51. |
Vancouver | Dülger D,Ekici S,ALBUZ O,Pakdemirli A Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR. Etlik Veteriner Mikrobiyoloji Dergisi. 2020; 31(1): 47 - 51. |
IEEE | Dülger D,Ekici S,ALBUZ O,Pakdemirli A "Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR." Etlik Veteriner Mikrobiyoloji Dergisi, 31, ss.47 - 51, 2020. |
ISNAD | Dülger, Dilek vd. "Investigation of Nasal Staphylococcus aureus carriage in hospital employees and rapid detection of PVL and mecA genes by RT-PCR". Etlik Veteriner Mikrobiyoloji Dergisi 31/1 (2020), 47-51. |