Objective: This study focused on assessing nurses’ emotional labor levels. Method: This descriptive study was done with 208 nurses who worked at intensive care service and inpatient services of a university hospital. The data were collected using Information Request Form and Emotional Labor Scale and were analyzed with percentages, arithmetical means, ANOVA, Kruskall-Wallis, Student’s t or Mann-Whitney U test. Results: Nurses received an average score of 2.80±0.68 from total emotional labor scale. As for subdimensions, nurses received 3.29±0.88 in deep acting subdimension, 3.23±0.77 in emotional effort subdimension and 2.26±0.86 in surface acting subdimension. Nurses who were aged ≥36 years and served for 1-10 patients per a day had statistically significantly higher scores in surface acting subdimension and total emotional labor scale than others. Besides, scores of emotional effort subdimension of those nurses who were married and scores of deep acting subdimension of those nurses who were satisfied with managers were statistically significantly higher than others; which was (p<0.05). Conclusion: Nurses use moderate level of emotional labor while they are caring for their patients and they demonstrate more surface acting and use more emotional labor as their ages increase and when they care for less than 10 patients every day.