Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi
Yıl: 2019 Cilt: 44 Sayı: Ek Sayı 1 Sayfa Aralığı: 434 - 446 Metin Dili: Türkçe DOI: 10.17826/cumj.573200 İndeks Tarihi: 09-12-2020
Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi
Öz: Amaç: Bu çalışmada Asherman sendromundaki (AS)endometriyal hasarlarda cerrahi yöntemlere ve medikaltedavilere alternatif olarak kök hücre tedavisinin etkileriaraştırılmıştır.Gereç ve Yöntem: Bu çalışmada sıçanlarda AS modelikimyasal olarak oluşturuldu. Aynı türün erkek bireylerinintibia ve femur kemiğinden izole edilen kemik iliği kaynaklımezenkimal kök hücreler (KİMKH) AS oluşturulan dişisıçanlara verilerek endometriyumda meydana gelendeğişiklikler histopatolojik parametrelerle değerlendirildi.Asherman+ besiyeri, Asherman+ niş, Asherman+KİMKH, Asherman+ KİMKH+ niş olmak üzere toplamdört grup oluşturuldu.Bulgular: Tedavi gruplarında epitel ve laminapropriyadaki rejenerasyonla birilkte endometriyalkalınlığın, bez sayısının ve vaskülarizasyonun arttığı, fibrözalanların ve apoptotik hücre ölümünün azaldığıgözlenmiştir. Kontrol grubu olarak değerlendirilen sağuterin hornlarda ise her hangi bir histopatolojik değişiklikgörülmemiştir.Sonuç: KİMKH ve Niş uygulamalarının, infertiliteyeneden olan mekanizmaların içerisinde yer alan adezyonoluşumunu azaltarakedavi ile kliniğe katkı sağlayabileceğigösterildi. Bu olumlu sonuçlar Asherman çalışmalarınınkliniğe taşınabilmesi açısından ümit vericidir.
Anahtar Kelime: Antiapoptotic and proliferative effect of bone marrow-derived mesenchymal stem cells on experimental Asherman model
Öz: Purpose: We investigated the effects of stem cell therapy as an alternative to surgical methods and medical treatments in endometrial injuries in Asherman syndrome (AS). Materials and Methods: In this study, AS model was created chemically in rats. The bone marrow-derived mesenchymal stem cells isolated from the tibia and femoral bone of male individuals of the same species (BMDSC) were given to female rats with asherman syndrome and the changes in the endometrium were evaluated by histopathological parameters. Asherman + medium, Asherman + niche, Asherman + BMDSCs, Asherman + BMDSCs + niche were formed in four groups. Results: It was observed that increased endometrial thickness, gland count and vascularization and decreased fibrous areas and apoptotic cell death with regeneration in epithelium and lamina propria in treatment groups. No histopathologic changes were observed in the right uterine horns, which were evaluated as control group. . Conclusion: BMDSCs and Niche applications can contribute to the clinic by reducing the formation of adhesion within the mechanisms causing infertility. These positive results are promising in terms of transporting Asherman studies to the clinic.It has been shown that BMDSCs and Niche may contribute to the clinic by treatment with adhesion molecules in mechanisms that cause infertility.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÖZTÜRK Ş, Kılıçaslan P, OZDEMIR I, TOPDAĞI Y, Tuğlu M (2019). Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. , 434 - 446. 10.17826/cumj.573200 |
| Chicago | ÖZTÜRK Şamil,Kılıçaslan Pınar,OZDEMIR ILHAN,TOPDAĞI YUNUS EMRE,Tuğlu Mehmet İbrahim Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. (2019): 434 - 446. 10.17826/cumj.573200 |
| MLA | ÖZTÜRK Şamil,Kılıçaslan Pınar,OZDEMIR ILHAN,TOPDAĞI YUNUS EMRE,Tuğlu Mehmet İbrahim Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. , 2019, ss.434 - 446. 10.17826/cumj.573200 |
| AMA | ÖZTÜRK Ş,Kılıçaslan P,OZDEMIR I,TOPDAĞI Y,Tuğlu M Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. . 2019; 434 - 446. 10.17826/cumj.573200 |
| Vancouver | ÖZTÜRK Ş,Kılıçaslan P,OZDEMIR I,TOPDAĞI Y,Tuğlu M Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. . 2019; 434 - 446. 10.17826/cumj.573200 |
| IEEE | ÖZTÜRK Ş,Kılıçaslan P,OZDEMIR I,TOPDAĞI Y,Tuğlu M "Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi." , ss.434 - 446, 2019. 10.17826/cumj.573200 |
| ISNAD | ÖZTÜRK, Şamil vd. "Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi". (2019), 434-446. https://doi.org/10.17826/cumj.573200 |
| APA | ÖZTÜRK Ş, Kılıçaslan P, OZDEMIR I, TOPDAĞI Y, Tuğlu M (2019). Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. Cukurova Medical Journal, 44(Ek Sayı 1), 434 - 446. 10.17826/cumj.573200 |
| Chicago | ÖZTÜRK Şamil,Kılıçaslan Pınar,OZDEMIR ILHAN,TOPDAĞI YUNUS EMRE,Tuğlu Mehmet İbrahim Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. Cukurova Medical Journal 44, no.Ek Sayı 1 (2019): 434 - 446. 10.17826/cumj.573200 |
| MLA | ÖZTÜRK Şamil,Kılıçaslan Pınar,OZDEMIR ILHAN,TOPDAĞI YUNUS EMRE,Tuğlu Mehmet İbrahim Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. Cukurova Medical Journal, vol.44, no.Ek Sayı 1, 2019, ss.434 - 446. 10.17826/cumj.573200 |
| AMA | ÖZTÜRK Ş,Kılıçaslan P,OZDEMIR I,TOPDAĞI Y,Tuğlu M Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. Cukurova Medical Journal. 2019; 44(Ek Sayı 1): 434 - 446. 10.17826/cumj.573200 |
| Vancouver | ÖZTÜRK Ş,Kılıçaslan P,OZDEMIR I,TOPDAĞI Y,Tuğlu M Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi. Cukurova Medical Journal. 2019; 44(Ek Sayı 1): 434 - 446. 10.17826/cumj.573200 |
| IEEE | ÖZTÜRK Ş,Kılıçaslan P,OZDEMIR I,TOPDAĞI Y,Tuğlu M "Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi." Cukurova Medical Journal, 44, ss.434 - 446, 2019. 10.17826/cumj.573200 |
| ISNAD | ÖZTÜRK, Şamil vd. "Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi". Cukurova Medical Journal 44/Ek Sayı 1 (2019), 434-446. https://doi.org/10.17826/cumj.573200 |
