Yıl: 2020 Cilt: 7 Sayı: 1 Sayfa Aralığı: 55 - 64 Metin Dili: İngilizce DOI: 10.34087/cbusbed.543107 İndeks Tarihi: 09-12-2020

Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury

Öz:
Objective: This study was aimed to determine the antioxidant and soft tissue wound healing properties ofpretreatment (p) of topical Hypericum perforatum (HP) oil in rabbits.Materials and Methods: Thirty-six New Zealand albino rabbits were randomly classified as p-HP oil (test, n = 18)and p-olive oil (control, n = 18) groups. Each groups of rabbits were topically pretreated with either HP or olive oilbefore the surgery. Gingival biopsies were excised and analyzed for re-epithelialization (RE), and granulation tissuematuration (GTM) and clinical appearance were scored for erythema, epithelial confluence and general woundappearance. Levels of VEGF and FGF-2 were assessed immunohistochemically. Tissue catalase (CAT) andmalondialdehyde (MDA) levels were measured using ELISA. All analyses were performed on days 3, 7 and 14 aftersurgery.Results: Higher epithelial confluence (on the 7th day) and general wound appearance scores (on the 7th and 14thdays) were determined in p-HP oil than that of p-olive oil group (p<0.05). On the other hand RE scores did notdiffer between groups (p>0.05). The number of positively stained FGF-2 cells was higher in the test group than thatof the control group (p<0.05), but there were no significant differences between the groups for the number ofpositively stained VEGF vessels. p-HP oil administration increased the tissue CAT levels and reduced the MDAlevels compared to p-olive oil (p<0.05).Conclusions: The results obtained from this study indicated that pretreatment with topical HP oil has antioxidanteffects and may be used to accelerate the secondary wound healing compared to its base, olive oil.
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  • 1.Orhan, IE, Kartal, et al. Inhibitory effect of St. Johns Wort oil macerates on TNFalpha-induced NF-kappaB activation and their fatty acid composition, Journal of Ethnopharmacology, 2014, 155, 1086-1092.
  • 2.Yadollah-Damavandi, S, Chavoshi-Nejad, et al. Topical Hypericum perforatum Improves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model, Evidence- Based Complementary and Alternative Medicine, 2015,article id:245328.
  • 3.Saddiqe, Z, Naeem, I, Maimoona, A, A review of the antibacterial activity of Hypericum perforatum L, Journal of Ethnopharmacology, 2010, 131, 511-521.
  • 4. Ozturk, N, Korkmaz, S, et al, Wound-healing activity of St. John's Wort (Hypericum perforatum L.) on chicken embryonic fibroblasts, Journal of Ethnopharmacology, 2007, 111, 33-39.
  • 5. Altiparmak, M, Eskitascioglu, T, Comparison of Systemic and Topical Hypericum Perforatum on Diabetic Surgical Wounds, Journal of Investigative Surgery, 2018, 31, 29-37.
  • 6. Samadi, S, Khadivzadeh, T, et al, The effect of Hypericum perforatum on the wound healing and scar of cesarean, The Journal of Alternative and Complementary Medicine, 2010, 16, 113-117.
  • 7. Lavagna SM, Secci D, et al, B, Efficacy of Hypericum and Calendula oils in the epithelial reconstruction of surgical wounds in childbirth with caesarean section, IL Farmaco, 2001, 56, 451-453.
  • 8. Mendonca, RJ, Coutinho-Netto, J, Cellular aspects of wound healing, Anais Brasileiros de Dermatologia, 2009, 84, 257-262.
  • 9. Guo, S, Dipietro, LA, Factors affecting wound healing, Journal of Dental Research, 2010, 89, 219-229.
  • 10. Dunnill, C, Patton, T, et al, Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROSmodulating technologies for augmentation of the healing process, International Wound Journal, 2017, 14, 89-96.
  • 11. Oberoi, SS, Dhingra, C, et al, Antibiotics in dental practice: how justified are we, International Dental Journal, 2015, 65, 4-10.
  • 12. Gunes, S, Tihminlioglu, F, Hypericum perforatum incorporated chitosan films as potential bioactive wound dressing material, International Journal of Biological Macromolecules, 2017, 102, 933- 943.
  • 13. Tanideh, N, Namazi, F, et al, Comparative assessment of the therapeutic effects of the topical and systemic forms of Hypericum perforatum extract on induced oral mucositis in golden hamsters, International Journal of Oral and Maxillofacial Surgery, 2014, 43, 1286-1292.
  • 14. Gunpinar, S, Kilic, OA, et al, Evaluation of the Effect of Topical Hypericum perforatum Oil on Excisional Palatal Wound Healing in Rabbits, Journal of Investigative Surgery, 2018, 1-10.
  • 15. Carr, AC, Maggini, S, Vitamin C and Immune Function, Nutrients, 2017, 9 (11), 1211.
  • 16. Neiva, RF, Steigenga, J, et al, Effects of specific nutrients on periodontal disease onset, progression and treatment, Journal of Clinical Periodontology, 2003, 30, 579-589.
  • 17. Slomiany, BL, Slomiany, A, Differential role of platelet-activating factor in gastric mucosal ulcer healing, Inflammopharmacology, 2003, 11, 237-248.
  • 18. Slomiany, BL, Slomiany, A, Biphasic role of platelet-activating factor in oral mucosal ulcer healing, IUBMB Life, 2003, 55, 483-490.
  • 19. Lawrence, T, Gilroy, DW, et al, Possible new role for NF-kB in the resolution of inflammation Nature Medicine, 2001, 7, 1291-1297.
  • 20. Aliasl, J, Khoshzaban, F, et al, Comparing the Healing Effects of Arnebia euchroma Ointment With Petrolatum on the Ulcers Caused by Fractional CO2 Laser: A Single-Blinded Clinical Trial, Iranian Red Crescent Medical Journal, 2014, 16, e16239.
  • 21. Li, K, Diao, Y, et al, Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats, BMC Complementary and Alternative Medicine, 2011, 11, 86.
  • 22. Nayak, SB, Isik, K, Marshall, JR, Wound-Healing Potential of Oil of Hypercium perforatum in Excision Wounds of Male Sprague Dawley Rats, Advances in Wound Care (New Rochelle), 2017, 6, 401-406.
  • 23. Schempp, CM, Winghofer, B, et al, Topical application of St John's wort (Hypericum perforatum L.) and of its metabolite hyperforin inhibits the allostimulatory capacity of epidermal cells, British Journal of Dermatology, 2000, 142, 979-984.
  • 24. Albert, D, Zundorf, I, et al, Hyperforin is a dual inhibitor of cyclooxygenase-1 and 5-lipoxygenase, Biochemical Pharmacology, 2002, 64, 1767-1775.
  • 25. Fuller, J, Muller-Goymann, CC, Anti-proliferative and antimigratory effects of hyperforin in 2D and 3D artificial constructs of human dermal fibroblasts - A new option for hypertrophic scar treatment?, European Journal of Pharmaceutics and Biopharmaceutics, 2018, 126, 108-114.
  • 26. Barrientos, S, Brem, H, et al, Clinical application of growth factors and cytokines in wound healing, Wound Repair and Regeneration, 2014, 22, 569-578.
  • 27. Przybylski, M, A review of the current research on the role of bFGF and VEGF in angiogenesis, Journal of Wound Care, 2009, 18, 516- 519.
  • 28. Zhang, X, Kang, X, et al, Stimulation of wound healing using bioinspired hydrogels with basic fibroblast growth factor (bFGF), International Journal of Nanomedicine, 2018, 13, 3897-3906.
  • 29. Khan, S, Villalobos, MA, et al, Fibroblast growth factor and vascular endothelial growth factor play a critical role in endotheliogenesis from human adipose-derived stem cells, Journal of Vascular Surgery, 2017, 65, 1483-1492.
  • 30. Kobayashi, F, Matsuzaka, K, Inoue T, The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands, International Journal of Oral Science, 2016, 8, 16-23.
  • 31. Murakami, S, Takayama, S, et al, Recombinant human basic fibroblast growth factor (bFGF) stimulates periodontal regeneration in class II furcation defects created in beagle dogs, Journal of Periodontal Research, 2003, 38, 97-103.
  • 32. Kiyan, HT, Demirci, B, et al, The in vivo evaluation of antiangiogenic effects of Hypericum essential oils using the chorioallantoic membrane assay, Pharmaceutical Biology, 2014, 52, 44-50.
  • 33. Billard, C, Merhi, F, Bauvois, B, Mechanistic insights into the antileukemic activity of hyperforin, Current Cancer Drug Targets, 2013, 13, 1-10.
  • 34. Dell'Aica, I, Niero, R, et al, Hyperforin blocks neutrophil activation of matrix metalloproteinase-9, motility and recruitment, and restrains inflammation-triggered angiogenesis and lung fibrosis, The Journal of Pharmacology and Experimental Therapeutics, 2007, 321, 492- 500.
  • 35. Tassone, E, Maran, C, et al, Antidepressant hyperforin up-regulates VEGF in CNS tumour cells, Pharmacological Research, 2011, 63, 37-43.
  • 36. Heinrich, M, Lorenz, P, et al, Lipid and Phenolic Constituents from Seeds of Hypericum perforatum L. and Hypericum tetrapterum Fr. and their Antioxidant Activity, Chemistry Biodiversity, 2017, 14 (8).
  • 37. Suzen, A, Tekin, L, et al, Protective Effects of Hypericum perforatum and Quercetin in a Rat Model of Ischemia/Reperfusion Injury of Testes, European Journal of Pediatric Surgery, 2018, 28, 96- 100.
  • 38. Abd El Motteleb, DM, Abd El Aleem, DI, Renoprotective effect of Hypericum perforatum against diabetic nephropathy in rats: Insights in the underlying mechanisms, Clinical and Experimental Pharmacology and Physiology, 2017, 44, 509-521.
  • 39. Ghosian, Moghaddam, MH, Roghani, M, Maleki M, Effect of Hypericum perforatum Aqueous Extracts on Serum Lipids, Aminotransferases, and Lipid Peroxidation in Hyperlipidemic Rats, Research in Cardiovascular Medicine, 2016, 5, e31326.
  • 40. Cakir, M, Duzova, H, et al, The effect of hypericum perforatum on kidney ischemia/reperfusion damage, Renal Failure, 2017, 39, 385- 391.
  • 41. Naziroglu, M, Kutluhan, S, et al, Modulation of oxidative stress, apoptosis, and calcium entry in leukocytes of patients with multiple sclerosis by Hypericum perforatum, Nutritional Neuroscience, 2014, 17, 214-221.
APA Gunpinar S, Alperat Kilic O, Tosun M, Fırat T (2020). Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. , 55 - 64. 10.34087/cbusbed.543107
Chicago Gunpinar Sadiye,Alperat Kilic Olgu,Tosun Mehmet,Fırat Tülin Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. (2020): 55 - 64. 10.34087/cbusbed.543107
MLA Gunpinar Sadiye,Alperat Kilic Olgu,Tosun Mehmet,Fırat Tülin Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. , 2020, ss.55 - 64. 10.34087/cbusbed.543107
AMA Gunpinar S,Alperat Kilic O,Tosun M,Fırat T Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. . 2020; 55 - 64. 10.34087/cbusbed.543107
Vancouver Gunpinar S,Alperat Kilic O,Tosun M,Fırat T Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. . 2020; 55 - 64. 10.34087/cbusbed.543107
IEEE Gunpinar S,Alperat Kilic O,Tosun M,Fırat T "Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury." , ss.55 - 64, 2020. 10.34087/cbusbed.543107
ISNAD Gunpinar, Sadiye vd. "Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury". (2020), 55-64. https://doi.org/10.34087/cbusbed.543107
APA Gunpinar S, Alperat Kilic O, Tosun M, Fırat T (2020). Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 7(1), 55 - 64. 10.34087/cbusbed.543107
Chicago Gunpinar Sadiye,Alperat Kilic Olgu,Tosun Mehmet,Fırat Tülin Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 7, no.1 (2020): 55 - 64. 10.34087/cbusbed.543107
MLA Gunpinar Sadiye,Alperat Kilic Olgu,Tosun Mehmet,Fırat Tülin Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol.7, no.1, 2020, ss.55 - 64. 10.34087/cbusbed.543107
AMA Gunpinar S,Alperat Kilic O,Tosun M,Fırat T Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2020; 7(1): 55 - 64. 10.34087/cbusbed.543107
Vancouver Gunpinar S,Alperat Kilic O,Tosun M,Fırat T Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2020; 7(1): 55 - 64. 10.34087/cbusbed.543107
IEEE Gunpinar S,Alperat Kilic O,Tosun M,Fırat T "Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury." Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 7, ss.55 - 64, 2020. 10.34087/cbusbed.543107
ISNAD Gunpinar, Sadiye vd. "Antioxidant Characteristic of Pretreatment Hypericum Perforatum Oil Administration in a Rabbit Model of Palatal Mucosal Injury". Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 7/1 (2020), 55-64. https://doi.org/10.34087/cbusbed.543107