Investigation of effects of PALB2 genetic variations on breast cancer predisposition

YALIN, Ali Erdinç; Bilen, Müge Yüksel; Yalın, Serap; eroğlu, pelin; Çömelekoğlu, Ülkü; Çalıkuşu, Züleyha; Berkoz, Mehmet

doi:10.17826/cumj.634598

Investigation of effects of PALB2 genetic variations on breast cancer predisposition

Müge Yüksel Bilen, (Mersin Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Mersin, Türkiye)

Mehmet Berköz, (Van Yüzüncü Yıl Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Van, Türkiye)

Ali Erdinç Yalın, (Mersin Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Mersin, Türkiye)

Züleyha Çalıkuşu, (Acıbadem Mehmet Ali Aydınlar Üniversitesi, Tıp Fakültesi Acıbadem Adana Hastanesi, Tıbbi Onkoloji Kliniği, Adana, Türkiye)

Pelin Eroğlu, (Mersin Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Mersin, Türkiye)

Ülkü Çömelekoğlu, (Mersin Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Mersin, Türkiye)

Serap Yalın (Mersin Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Mersin, Türkiye)

Cukurova Medical Journal

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Yıl: 2020 Cilt: 45 Sayı: 1 Sayfa Aralığı: 186 - 194 Metin Dili: İngilizce DOI: 10.17826/cumj.634598 İndeks Tarihi: 10-12-2020

Investigation of effects of PALB2 genetic variations on breast cancer predisposition

Öz:

Purpose: In this study, the effects of three different singlenucleotide polymorphisms (rs249954, rs249935, andrs16940342) of partner and localizer of breast cancer gene2 (PALB2) on breast cancer predisposition have beeninvestigated.Materials and Methods: For this purpose, 150 patientsdiagnosed to have breast cancer and 150 healthyindividuals have been included. By using real timepolymerase chain reaction (PCR) method isolateddeoxyribonucleic acid (DNA) from each case has beeninvestigated for the PALB2 genetic variations.Results: The distribution of homozygote wild type (AA)and heterozygote (AG) genotypes at rs16940342polymorphism has been observed to be 44.7% and 55.3%in breast cancer group and 32.7% and 67.3% in controlgroup. The homozygote polymorphic (GG) genotype wasnot observed in both groups. The discrepancy between thegroups in terms of genotype distribution regardingrs16940342 polymorphism has been found statisticallysignificant. However, there was no significant difference inthe frequencies of rs249954 and rs249935 polymorphismscomparing both groups.Conclusion: These results show that rs16940342polymorphism may be an important determinant in termsof breast cancer predisposition in the Turkish population.

Anahtar Kelime:

PALB2 genetik varyasyonlarının meme kanseri yatkınlığı üzerindeki etkilerinin araştırılması

Öz:

Amaç: Bu çalışmada, meme kanseri geni partner ve lokalizeri-2’nin (PALB2) üç farklı tek nükleotid polimorfizminin (rs249954, rs249935 ve rs16940342) meme kanseri predispozisyonu üzerindeki etkileri araştırılmıştır. Gereç ve Yöntem: Bu amaçla, çalışmamıza meme kanseri tanısı almış 150 hasta ve 150 sağlıklı birey dahil edilmiştir. Gerçek zamanlı polimeraz zincir reaksiyonu (PCR) yöntemi kullanılarak, her bir vakadan izole edilmiş deoksiribonükleik asit (DNA), PALB2 genetik varyasyonları için araştırılmıştır. Bulgular: rs16940342 polimorfizminin homozigot yabanıl tip (AA) ve heterozigot (AG) genotip dağılımı meme kanseri grubunda sırasıyla % 44,7 ve % 55,3, kontrol grubunda ise % 32,7 ve % 67,3 olarak bulunmuştur. Her iki grupta da homozigot polimorfik (GG) genotip gözlenmemiştir. rs16940342 polimorfizmi için gruplar arasındaki genotip dağılımı açısından fark istatistiksel olarak anlamlı bulunmuştur. Bununla birlikte, her iki grup karşılaştırıldığında rs249954 ve rs249935 polimorfizmlerinin sıklığı bakımından istatistiksel olarak anlamlı bir fark bulunamamıştır. Sonuç: Bu sonuçlar rs16940342 polimorfizminin Türk popülasyonunda meme kanseri yatkınlığı açısından önemli bir belirleyici rol üstlenebileceğini göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Bilen M, Berkoz M, YALIN A, Çalıkuşu Z, eroğlu p, Çömelekoğlu Ü, Yalın S (2020). Investigation of effects of PALB2 genetic variations on breast cancer predisposition. , 186 - 194. 10.17826/cumj.634598
Chicago	Bilen Müge Yüksel,Berkoz Mehmet,YALIN Ali Erdinç,Çalıkuşu Züleyha,eroğlu pelin,Çömelekoğlu Ülkü,Yalın Serap Investigation of effects of PALB2 genetic variations on breast cancer predisposition. (2020): 186 - 194. 10.17826/cumj.634598
MLA	Bilen Müge Yüksel,Berkoz Mehmet,YALIN Ali Erdinç,Çalıkuşu Züleyha,eroğlu pelin,Çömelekoğlu Ülkü,Yalın Serap Investigation of effects of PALB2 genetic variations on breast cancer predisposition. , 2020, ss.186 - 194. 10.17826/cumj.634598
AMA	Bilen M,Berkoz M,YALIN A,Çalıkuşu Z,eroğlu p,Çömelekoğlu Ü,Yalın S Investigation of effects of PALB2 genetic variations on breast cancer predisposition. . 2020; 186 - 194. 10.17826/cumj.634598
Vancouver	Bilen M,Berkoz M,YALIN A,Çalıkuşu Z,eroğlu p,Çömelekoğlu Ü,Yalın S Investigation of effects of PALB2 genetic variations on breast cancer predisposition. . 2020; 186 - 194. 10.17826/cumj.634598
IEEE	Bilen M,Berkoz M,YALIN A,Çalıkuşu Z,eroğlu p,Çömelekoğlu Ü,Yalın S "Investigation of effects of PALB2 genetic variations on breast cancer predisposition." , ss.186 - 194, 2020. 10.17826/cumj.634598
ISNAD	Bilen, Müge Yüksel vd. "Investigation of effects of PALB2 genetic variations on breast cancer predisposition". (2020), 186-194. https://doi.org/10.17826/cumj.634598

APA	Bilen M, Berkoz M, YALIN A, Çalıkuşu Z, eroğlu p, Çömelekoğlu Ü, Yalın S (2020). Investigation of effects of PALB2 genetic variations on breast cancer predisposition. Cukurova Medical Journal, 45(1), 186 - 194. 10.17826/cumj.634598
Chicago	Bilen Müge Yüksel,Berkoz Mehmet,YALIN Ali Erdinç,Çalıkuşu Züleyha,eroğlu pelin,Çömelekoğlu Ülkü,Yalın Serap Investigation of effects of PALB2 genetic variations on breast cancer predisposition. Cukurova Medical Journal 45, no.1 (2020): 186 - 194. 10.17826/cumj.634598
MLA	Bilen Müge Yüksel,Berkoz Mehmet,YALIN Ali Erdinç,Çalıkuşu Züleyha,eroğlu pelin,Çömelekoğlu Ülkü,Yalın Serap Investigation of effects of PALB2 genetic variations on breast cancer predisposition. Cukurova Medical Journal, vol.45, no.1, 2020, ss.186 - 194. 10.17826/cumj.634598
AMA	Bilen M,Berkoz M,YALIN A,Çalıkuşu Z,eroğlu p,Çömelekoğlu Ü,Yalın S Investigation of effects of PALB2 genetic variations on breast cancer predisposition. Cukurova Medical Journal. 2020; 45(1): 186 - 194. 10.17826/cumj.634598
Vancouver	Bilen M,Berkoz M,YALIN A,Çalıkuşu Z,eroğlu p,Çömelekoğlu Ü,Yalın S Investigation of effects of PALB2 genetic variations on breast cancer predisposition. Cukurova Medical Journal. 2020; 45(1): 186 - 194. 10.17826/cumj.634598
IEEE	Bilen M,Berkoz M,YALIN A,Çalıkuşu Z,eroğlu p,Çömelekoğlu Ü,Yalın S "Investigation of effects of PALB2 genetic variations on breast cancer predisposition." Cukurova Medical Journal, 45, ss.186 - 194, 2020. 10.17826/cumj.634598
ISNAD	Bilen, Müge Yüksel vd. "Investigation of effects of PALB2 genetic variations on breast cancer predisposition". Cukurova Medical Journal 45/1 (2020), 186-194. https://doi.org/10.17826/cumj.634598