BACKGROUND/AIMSTemporomandibular joint (TMJ) hypermobility is characterized by condyle hypertranslation, which moves anteriorly to the articular eminence as the mouth opens. This study investigated the efficacy of organic silicon prolotherapy for treating TMJ hypermobility.MATERIAL and METHODSThe study sample consisted of six young New Zealand White rabbits. One of the rabbit’s TMJ’s was injected with organic silicon while the other joint was injected with isotonic saline. Isotonic saline (1.5 mL) was bilaterally administered into the upper joint space (1 mL) and pericapsular tissue (0.5 mL). The rabbits were sacrificed using high-dose anesthetics one month after the follow-up. The sample sections were stained with Mallory-Azan dye in order to identify collagen fibers. The samples were analyzed in terms of fibrosis and tissue reactions. Statistical analysis was performed using the Mann-Whitney U test.RESULTSThere was a significant difference in the severity of fibrosis amongst the study groups (p=0.00512). It was noted that more collagen fibers and adipose tissue were produced from organic silicon than saline in the retrodiscal ligament.CONCLUSIONThe study concluded that organic silicon could be used as an alternative to dextrose injection for prolotherapy. Future studies and clinical trials are necessary to gain further insight.