Yıl: 2020 Cilt: 5 Sayı: 2 Sayfa Aralığı: 153 - 156 Metin Dili: İngilizce DOI: 10.5152/cjms.2020.1542 İndeks Tarihi: 10-12-2020

Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia

Öz:
BACKGROUND/AIMSThe purpose of this study was to research the effects of genistein on telomerase activity and apoptosis in an acute promyelocytic leukemia cell line (HL-60).MATERIAL and METHODSIn HL-60 cells, the cytotoxic effect of commercially available genistein was evaluated by the XTT method. The XTT method is a Cell Proliferation Assay. The Annexin V-EGFP method was used to determine apoptosis. The human telomerase reverse transcriptase (hTERT) is a marker of telomerase activity. hTERT gene expression analysis was performed by LightCycler real-time RT-PCR.RESULTSIn HL-60 cells, the IC50 of genistein was found to be 50 μM at 72 hours. It was observed that the induction of apoptosis was 4.25-fold higher compared to the genistein untreated cells used as the control group. Compared to the control group, hTERT activity was found to decrease by 5.16, 3.81 and 5.04-fold at 24, 48 and 72 hours, respectively.CONCLUSIONInduced apoptosis of HL-60 cells by the reduction of human telomerase reverse transcriptase activity may be a beneficial parameter in leukemia patients
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APA OKÇANOĞLU T, BIRAY AVCI C, YILMAZ SUSLUER S, Kayabasi C, Saydam G, Gunduz C (2020). Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. , 153 - 156. 10.5152/cjms.2020.1542
Chicago OKÇANOĞLU Tuğçe BALCI,BIRAY AVCI Cigir,YILMAZ SUSLUER SUNDE,Kayabasi Cagla,Saydam Guray,Gunduz Cumhur Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. (2020): 153 - 156. 10.5152/cjms.2020.1542
MLA OKÇANOĞLU Tuğçe BALCI,BIRAY AVCI Cigir,YILMAZ SUSLUER SUNDE,Kayabasi Cagla,Saydam Guray,Gunduz Cumhur Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. , 2020, ss.153 - 156. 10.5152/cjms.2020.1542
AMA OKÇANOĞLU T,BIRAY AVCI C,YILMAZ SUSLUER S,Kayabasi C,Saydam G,Gunduz C Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. . 2020; 153 - 156. 10.5152/cjms.2020.1542
Vancouver OKÇANOĞLU T,BIRAY AVCI C,YILMAZ SUSLUER S,Kayabasi C,Saydam G,Gunduz C Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. . 2020; 153 - 156. 10.5152/cjms.2020.1542
IEEE OKÇANOĞLU T,BIRAY AVCI C,YILMAZ SUSLUER S,Kayabasi C,Saydam G,Gunduz C "Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia." , ss.153 - 156, 2020. 10.5152/cjms.2020.1542
ISNAD OKÇANOĞLU, Tuğçe BALCI vd. "Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia". (2020), 153-156. https://doi.org/10.5152/cjms.2020.1542
APA OKÇANOĞLU T, BIRAY AVCI C, YILMAZ SUSLUER S, Kayabasi C, Saydam G, Gunduz C (2020). Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. Cyprus Journal of Medical Sciences, 5(2), 153 - 156. 10.5152/cjms.2020.1542
Chicago OKÇANOĞLU Tuğçe BALCI,BIRAY AVCI Cigir,YILMAZ SUSLUER SUNDE,Kayabasi Cagla,Saydam Guray,Gunduz Cumhur Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. Cyprus Journal of Medical Sciences 5, no.2 (2020): 153 - 156. 10.5152/cjms.2020.1542
MLA OKÇANOĞLU Tuğçe BALCI,BIRAY AVCI Cigir,YILMAZ SUSLUER SUNDE,Kayabasi Cagla,Saydam Guray,Gunduz Cumhur Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. Cyprus Journal of Medical Sciences, vol.5, no.2, 2020, ss.153 - 156. 10.5152/cjms.2020.1542
AMA OKÇANOĞLU T,BIRAY AVCI C,YILMAZ SUSLUER S,Kayabasi C,Saydam G,Gunduz C Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. Cyprus Journal of Medical Sciences. 2020; 5(2): 153 - 156. 10.5152/cjms.2020.1542
Vancouver OKÇANOĞLU T,BIRAY AVCI C,YILMAZ SUSLUER S,Kayabasi C,Saydam G,Gunduz C Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. Cyprus Journal of Medical Sciences. 2020; 5(2): 153 - 156. 10.5152/cjms.2020.1542
IEEE OKÇANOĞLU T,BIRAY AVCI C,YILMAZ SUSLUER S,Kayabasi C,Saydam G,Gunduz C "Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia." Cyprus Journal of Medical Sciences, 5, ss.153 - 156, 2020. 10.5152/cjms.2020.1542
ISNAD OKÇANOĞLU, Tuğçe BALCI vd. "Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia". Cyprus Journal of Medical Sciences 5/2 (2020), 153-156. https://doi.org/10.5152/cjms.2020.1542