Purpose: We aimed to determine the antibiotic susceptibility and distribution of factors leading to hospital-acquired infections in our hospital. Patients and Methods: In our hospital with a capacity of approximately 116 beds, hospital-acquired infections between January 2017 and December 2017 were evaluated retrospectively by the surveillance method. Results: 9931 patients were hospitalized in Acıbadem Adana Hospital between January 2017 and December 2017. Hospitalacquired infections (HAI) have been detected in 92 patients. The infection rate in the hospital was 1.32%. The ratio of HAI was determined as bacteremia in 30 patients (29.4%), surgical site infection in 22 patients (21.5%), urinary tract infection in 20 patients (19.6%), and pneumonia in 15 patients (14.7%). The two most common HAI in the pediatric age group were bacteremia and pneumonia while the urinary tract and surgical site infection were common in the adult age group. One or more agents were isolated in 97.7% of patients with HAI, while no agents were isolated in 2.3% of patients. The most common microorganisms isolated in the hospital were Klebsiella spp. (29.5%), E.coli (22.7%), Yeasts 20.5% and S.aureus (9%), respectively. HAI was mostly seen in oncology service (57.8%), general intensive care unit (24.5%) and pediatric bone marrow unit (17.6%). Conclusion: HAI is one of the most important indicators of the service quality of inpatient treatment institutions/centers and is seen as an important health problem all over the world. All hospitals and units should determine the flora and resistance rate of their patient profile by surveillance studies. Besides, treatment decisions should be given accordingly. Surveillance studies should be performed to prevent HAI and the results should be monitored regularly. Also, unnecessary invasive procedures should not be allowed.