Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis

Zenginkinet, Tülay; Özdamar, Osman İlkay; Acar, Gul Özbilen; Ozen, Filiz

doi:10.32448/entupdates.711666

Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis

Osman Ilkay OZDAMAR, (İstanbul Medeniyet Üniversitesi, Göztepe Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz ve Baş Boyun Cerrahisi Ana Bilim Dalı, İstanbul, Türkiye)

Gul OZBILEN ACAR, (İstanbul Medeniyet Üniversitesi, Göztepe Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz ve Baş Boyun Cerrahisi Ana Bilim Dalı, İstanbul, Türkiye)

Filiz ÖZEN, (İstanbul Medeniyet Üniversitesi, Göztepe Eğitim ve Araştırma Hastanesi, Tıbbi Genetik Bölümü, İstanbul, Türkiye)

Tülay ZENGİNKİNET (İstanbul Medeniyet Üniversitesi, Göztepe Eğitim ve Araştırma Hastanesi, Patoloji Kliniği, İstanbul, Türkiye)

ENT Updates

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Yıl: 2020 Cilt: 10 Sayı: 2 Sayfa Aralığı: 300 - 305 Metin Dili: İngilizce DOI: 10.32448/entupdates.711666 İndeks Tarihi: 12-12-2020

Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis

Öz:

Objective: The present study compared three studygroups composed of patients with only papillary thyroid carcinoma (PTC), patients with only Hashimoto’sthyroiditis (HT) and patients with PTC+HT in terms ofBRAF-V600E, KRAS, NRAS and epidermal growth factorreceptor (EGFR) mutations. Also, the association betweenclinicopathological prognostic indicators including tumormultifocality, extrathyroidal extension (ETE), lymph node(LN) metastasis and recurrence and BRAF-V600E mutations were investigated in the PTC and PTC+HT groups.Methods: A total of 53 patients (two males and 51 females) who underwent a hemi/total thyroidectomy dueto suspicion of malignancy or malignant lesion accordingto the thyroid cytopathology participated in the study.The study groups were composed of 19 patients withPTC, 18 with PTC+HT and 16 with HT according to thehistopathological examination records. Histopathologicalsections from the paraffin blocks of the patients were investigated for BRAF-V600E, KRAS, NRAS and EGFR genemutations using real-time polymerase chain reaction(PCR) assay.Results: There was no significant difference between thegroups in terms of BRAF-V600E KRAS, NRAS and EGFRmutation rates. Also, presence of the BRAF-V600E mutation was not correlated with the prognostic indicators forthe patients with PTC and PTC+HT.Conclusion: In the present study, no significant association was found between PTC and HT, and the BRAFV600E, KRAS, NRAS and EGFR mutations. Further studies with a larger number of patients may help to clarifythe clinicopathological and diagnostic importance of theBRAF-V600E, KRAS, NRAS and EGFR mutations in thyroiddiseases.

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APA	Özdamar O, Acar G, Ozen F, Zenginkinet T (2020). Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. , 300 - 305. 10.32448/entupdates.711666
Chicago	Özdamar Osman İlkay,Acar Gul Özbilen,Ozen Filiz,Zenginkinet Tülay Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. (2020): 300 - 305. 10.32448/entupdates.711666
MLA	Özdamar Osman İlkay,Acar Gul Özbilen,Ozen Filiz,Zenginkinet Tülay Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. , 2020, ss.300 - 305. 10.32448/entupdates.711666
AMA	Özdamar O,Acar G,Ozen F,Zenginkinet T Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. . 2020; 300 - 305. 10.32448/entupdates.711666
Vancouver	Özdamar O,Acar G,Ozen F,Zenginkinet T Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. . 2020; 300 - 305. 10.32448/entupdates.711666
IEEE	Özdamar O,Acar G,Ozen F,Zenginkinet T "Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis." , ss.300 - 305, 2020. 10.32448/entupdates.711666
ISNAD	Özdamar, Osman İlkay vd. "Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis". (2020), 300-305. https://doi.org/10.32448/entupdates.711666

APA	Özdamar O, Acar G, Ozen F, Zenginkinet T (2020). Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. ENT Updates, 10(2), 300 - 305. 10.32448/entupdates.711666
Chicago	Özdamar Osman İlkay,Acar Gul Özbilen,Ozen Filiz,Zenginkinet Tülay Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. ENT Updates 10, no.2 (2020): 300 - 305. 10.32448/entupdates.711666
MLA	Özdamar Osman İlkay,Acar Gul Özbilen,Ozen Filiz,Zenginkinet Tülay Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. ENT Updates, vol.10, no.2, 2020, ss.300 - 305. 10.32448/entupdates.711666
AMA	Özdamar O,Acar G,Ozen F,Zenginkinet T Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. ENT Updates. 2020; 10(2): 300 - 305. 10.32448/entupdates.711666
Vancouver	Özdamar O,Acar G,Ozen F,Zenginkinet T Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis. ENT Updates. 2020; 10(2): 300 - 305. 10.32448/entupdates.711666
IEEE	Özdamar O,Acar G,Ozen F,Zenginkinet T "Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis." ENT Updates, 10, ss.300 - 305, 2020. 10.32448/entupdates.711666
ISNAD	Özdamar, Osman İlkay vd. "Assessment of BRAF-V600E, KRAS, NRAS and EGFR mutations in papillary thyroid carcinoma and Hashimoto’s thyroiditis". ENT Updates 10/2 (2020), 300-305. https://doi.org/10.32448/entupdates.711666