Health benefits of lady finger were explored in this study by which the ethanol extract from theseeds was utilized for testing. In high glucose (350 mmole/L) medium, the proliferative activity ofC2C12 cells was inferior, but their viability was improved following treated with the extract. Theactivities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were also enhanced. In response to such high glucose concentration, glucose uptake ability of these cells wascompromised. The extract was demonstrated to increase the ability of glucose uptake by approximately equal to the effect of 200 nmole/L insulin. High intracellular reactive oxygen species (ROS)of RBM-MCS cells grown in high glucose containing medium was elicited. The level of ROS wasreduced when the cells were cultured in contact with the extract. L929 cells were made inflammation and oxidative stress by incubating with 37.5 µmole/L DPPH for 30 min. The release of tumornecrosis factor-α (TNF-α) and interleukin 6 (IL-6) from these inflamed cells was inhibited by suchtreatment. Thus, lady finger might be advantageous in the protection of cells in the body that wouldbe damaged or being susceptible to hyperglycemia and diabetes.