In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog
Yıl: 2020 Cilt: 36 Sayı: 1 Sayfa Aralığı: 28 - 35 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2020.256 İndeks Tarihi: 26-11-2020
In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog
Öz: Aim: The major problem of drug intended for tick control is resistancedeveloped to application doses on the hosts. The acaricidal effectsof alkaloids from Glaucium flavum and Peganum harmala wereevaluated in vitro on adult female of Rhipicephalus sp. collected innaturally infected dogs.Materials and Methods: The acaricide activities of the both extracts(3.12, 6.2, 12.5, 25 and 50 mg/ml) were evaluated using theadult immersion test, reproductive index and inhibition oviposition.DMSO (1%) and SEB (1μl/ml) were used as a negative and positivecontrol.Results: The percent mortality caused by the methanolic extractsof both plants varied from 41.66 to 75 %, when tested at concentrationsranging from 3.12 to 50 mg/ml, and significantly differentcompared to control (p<0.05). Visually hatching of the eggs wascompletely blocked only by G. flavum with 50 mg/ml; however, otherextracts of both plants were partially able to block the hatching. Theoviposition and reproductive rate of both plants were concentrationdependent in both plants and significantly different (p<0.05) comparedto negative control. Also, the extracts of P. harmala seem to bemore efficient against Rhipicephalus sp. adults at different concentrationsthan the extracts G. flavum. The alkaloid extracts of both plantshigh effects were observed with 6.25 and 50 mg/ml for G. flavum andwith 25mg/ml and 50mg/ml for P. harmala.Conclusion: The total alkaloids extracted from the G. flavum rootand P. harmala seed have good acaricidal activities in vitro reducingpotentially the egg laying of Rhipicephalus sp. females.
Anahtar Kelime: Köpeklerde Rhipicephalus’a (sp) karşı Peganum harmala ve Glaucium flavum alkaloidinin in vitro akarisit aktivitesi
Öz: Amaç: Kene kontrolü için amaçlanan temel ilaç sorunu konakçılar üzerinde uygulama dozuna gelişen dirençtir. Glaucium flavum ve Peganum harmala'dan alkaloitlerin akarisit etkileri doğal ortamda enfekte olan köpeklerden toplanan invitro olarak yetişkin dişi Rhipicephalus sp türünde değerlendirildi. Gereç ve Yöntem: Her iki ekstraktın akarisit faaliyetleri (3.12, 6.2, 12.5, 25 ve 50 mg / ml), erişkin daldırma testi, üreme indeksi ve inhibisyon yumurtlama kullanılarak değerlendirildi. Bulgular: Metanolik ekstraktların neden olduğu mortalite yüzdesi her iki bitkinin % 3.66 ila 50 mg / ml arasındaki konsantrasyonlarda test edildiğinde % 41.66 ila 75 arasında değişmekteydi ve kontrole kıyasla önemli ölçüde farklıydı (p<0.05). Görsel olarak yumurtaların yumurtadan çıkması tamamen 50 mg / ml ile G. flavum tarafından bloke edilmiştir, ancak diğer her iki bitkinin ekstraktları da yumurtadan çıkmayı kısmen engelleyebilmiştir. Her iki bitkinin yumurtlama ve üreme oranı konsantrasyona bağımlıydı ve negatif kontrole kıyasla önemli ölçüde farklıydı (p<0.05). Ayrıca, P. harmala ekstraktları G. flavum ekstraklarından Rhipicephalus sp türüne karşı daha etkili olduğu görünmüştür. Her iki bitkinin de alkaloid ekstrakları, G. flavum için 6.25 ve 50 mg/ml ve P. harmala için 25mg/ml ve 50mg / ml düzeyi ile gözlemlenmiştir. Öneri: G. flavum kökünden ve P. harmala tohumundan ekstrakte edilen toplam alkaloidler potansiyel olarak in vitro Rhipicephalus sp. yumurtlayan dişilerin azaltılmasında iyi akarisit aktivitelerinin olduğu gözlemlenmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Kebbi R, Besseboua O, Nait Mouloud M, Ayad A (2020). In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. , 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| Chicago | Kebbi Rosa,Besseboua Omar,Nait Mouloud Mohamed,Ayad Abdelhanine In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. (2020): 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| MLA | Kebbi Rosa,Besseboua Omar,Nait Mouloud Mohamed,Ayad Abdelhanine In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. , 2020, ss.28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| AMA | Kebbi R,Besseboua O,Nait Mouloud M,Ayad A In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. . 2020; 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| Vancouver | Kebbi R,Besseboua O,Nait Mouloud M,Ayad A In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. . 2020; 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| IEEE | Kebbi R,Besseboua O,Nait Mouloud M,Ayad A "In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog." , ss.28 - 35, 2020. 10.15312/EurasianJVetSci.2020.256 |
| ISNAD | Kebbi, Rosa vd. "In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog". (2020), 28-35. https://doi.org/10.15312/EurasianJVetSci.2020.256 |
| APA | Kebbi R, Besseboua O, Nait Mouloud M, Ayad A (2020). In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. Eurasian Journal of Veterinary Sciences, 36(1), 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| Chicago | Kebbi Rosa,Besseboua Omar,Nait Mouloud Mohamed,Ayad Abdelhanine In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. Eurasian Journal of Veterinary Sciences 36, no.1 (2020): 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| MLA | Kebbi Rosa,Besseboua Omar,Nait Mouloud Mohamed,Ayad Abdelhanine In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. Eurasian Journal of Veterinary Sciences, vol.36, no.1, 2020, ss.28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| AMA | Kebbi R,Besseboua O,Nait Mouloud M,Ayad A In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. Eurasian Journal of Veterinary Sciences. 2020; 36(1): 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| Vancouver | Kebbi R,Besseboua O,Nait Mouloud M,Ayad A In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog. Eurasian Journal of Veterinary Sciences. 2020; 36(1): 28 - 35. 10.15312/EurasianJVetSci.2020.256 |
| IEEE | Kebbi R,Besseboua O,Nait Mouloud M,Ayad A "In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog." Eurasian Journal of Veterinary Sciences, 36, ss.28 - 35, 2020. 10.15312/EurasianJVetSci.2020.256 |
| ISNAD | Kebbi, Rosa vd. "In-vitro acaricidal activity of Peganum harmala and Glaucium flavum alkaloid against Rhipicephalus sp. of dog". Eurasian Journal of Veterinary Sciences 36/1 (2020), 28-35. https://doi.org/10.15312/EurasianJVetSci.2020.256 |
