Papillomavirus is a viral agent that induces the formation ofbenign tumors in mammals. There are different treatmentmethods for cutaneous papilloma caused by the bovinepapillomavirus (BPV), and one of them is autogenous vaccination. We aimed to investigate the immunological effects ofautogenous vaccination in cattle. In this study, 30 cattle withcutaneous papillomatosis were included. The diagnosis wasmade by histopathology and polymerase chain reaction, anda phylogenetic tree was constructed. As a result of the phylogenetic analyses, the pathogen responsible for the papillomawas classified as BPV-2 type in the genus of Delta papillomavirus. The papilloma tissue specimens collected from eachanimal were used to prepare the autogenous vaccine. The autogenous vaccine was administered once a week for 4 weeks.The blood samples collected before and after the vaccinationwere immunologically analyzed. It was determined that theautogenous vaccination led to an increase in the leukocyte,lymphocyte, and interleukin-6 (IL-6) levels. Once the vaccinewas administered, regression started in the cutaneous papilloma in the first month. As a result, bovine papillomatosis isan economically important infection for dairy farming. It hasbeen concluded that autogenous vaccination is a practicaltherapeutic method that causes lesions to regress, especiallyby triggering IL-6 and lymphocyte production in the cattle infected with papillomavirus