Yıl: 2020 Cilt: 47 Sayı: 2 Sayfa Aralığı: 278 - 285 Metin Dili: İngilizce DOI: 10.5798/dicletip.755702 İndeks Tarihi: 28-11-2020

A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids

Öz:
Objective: Total white blood cell (WBC) count in body fluids (BF) is important in diagnosis and treatment of inflammatoryand infectious diseases. We aimed to compare hemogram and BF modes of Mindray BC-6800 hematology analyzer withthe manual hemocytometry method for determination of total WBC count in peritoneal and pleural fluids.Method: Our study consisted of a total of 143 specimens, 109 peritoneal fluid and 34 pleural effusion fluid. Each samplewas analyzed consecutively twice, first with the manual method and, later with both hemogram mode and the BF modeof Mindray BC-6800 automated hematology analyzer.Results: There was a statistically significant difference between manual count and hemogram mode (for peritoneal fluidsp< 0.001, for pleural fluids p< 0.001). There was a statistically significant difference between the hemogram mode andBF mode too. (for peritoneal fluids p< 0.001, for pleural fluids p< 0.001). However, there was no statistically significantdifference between the manual count and BF mode (for peritoneal fluids p=0.236, for pleural fluids p=0.627). Lessthan100 cells/mL, there was a statistically significant difference between each of the counting methods (Manual count vshemogram mode p< 0.001, manual count vs BF mode p= 0.012, hemogram mode vs BF mode p< 0.001).More than 100 cells/mL, there was no statistically significant difference between manual count and the BF mode (p=0.332). However, there were statistically significant differences between manual count and hemogram mode, andhemogram mode and BF mode (p= 0.003 and p< 0.001 respectively).Conclusion: The use of manual method instead of hematology analyzer is more convenient in samples <100 cells/mL,and BF mode may be used as a screening tool in samples >100 cells/mL.
Anahtar Kelime:

Peritoneal Ve Plevral Sıvılarda Hücre Sayımı İçin Manuel Hemasitometre İle Mindray BC6800 Hematoloji Analizörünün Farklı Modlarının Karşılaştırılması

Öz:
Amaç: Vücut sıvılarında (BF) toplam beyaz kan hücre (WBC) sayısı, enflamatuar ve bulaşıcı hastalıkların tanı ve tedavisinde önemlidir. Peritoneal ve plevral sıvılarda toplam WBC sayısının belirlenmesi için Mindray BC-6800 hematoloji analizörünün hemogram ve BF modlarını manuel hemositometri yöntemiyle karşılaştırmayı amaçladık. Yöntemler: Çalışmamız 109 periton sıvısı ve 34 plevral efüzyon sıvısı olmak üzere toplam 143 örnekten oluşuyordu. Herbir örnek, önce manuel yöntemle ve daha sonra Mindray BC-6800 otomatik hematoloji analizörünün hemogram ve BF modları ile iki kez art arda analiz edildi. Bulgular: Manuel sayım ve hemogram modu arasında istatistiksel olarak anlamlı farklılık vardı (periton sıvıları için p< 0.001, plevral sıvılar için p< 0.001). Hemogram modu ile BF modu arasında da istatistiksel olarak anlamlı bir fark bulundu (periton sıvıları için p< 0.001, plevral sıvılar için p< 0.001). Ancak manuel sayım ve BF modu arasında istatistiksel olarak anlamlı bir sonuç bulunamadı (Periton sıvıları için p= 0.236, plevral sıvılar için p= 0.627). 100 hücre/mL’den daha az olduğunda, her bir sayma yöntemi arasında istatistiksel olarak anlamlı farklılık mevcuttu (Manuel sayım & hemogram modu p< 0.001, manuel sayım & BF modu p= 0,012, hemogram modu & BF modu p< 0.001). 100 hücre/mL’den fazla olduğunda, manuel sayım ve BF modu arasında istatistiksel olarak anlamlı bir farklılık yoktu (p= 0,332). Ancak manuel sayım ve hemogram modu arasında ve hemogram modu ve BF modu arasında arasında istatistiksel olarak anlamlı bir farklılık vardı (sırasıyla p= 0,003 ve p< 0.001). Sonuç: Hematoloji analizörü yerine manuel metodun kullanılması, <100 hücre / mL'lik numunelerde daha uygundur ve BF modu > 100 hücre / mL'lik numunelerde bir tarama aracı olarak kullanılabilir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. De Smet D, Van Moer G, Martens GA, et al. Use of the Cell-Dyn Sapphire hematology analyzer for automated counting of blood cells in body fluids. Am J Clin Pathol. 2010; 133: 291-9.
  • 2. Cognialli RCR, Comar SR, Souza AMD, Singer GMB. Evaluation of pleural and ascitic fluid analysis on the Sysmex XE-5000 hematology analyzer. J Bras Patol Med Lab. 2017; 53; 150-8.
  • 3. Fuster O, Andino B, Pardo A, Laiz B. Continuous ambulatory peritoneal dialysis, ascitic and pleural body fluids evaluation with the Mindray BC-6800 hematology analyzer. Journal of clinical laboratory analysis. J Clin Lab Anal. 2018; 32: e22240.
  • 4. Runyon BA. Management of adult patients with ascites due to cirrhosis: an update. Hepatology. 2009; 49: 2087-107.
  • 5. Conner BD, Lee YG, Branca P, Rogers JT, Rodriguez RM, Light RW. Variations in pleural fluid WBC count and differential counts with different sample containers and different methods. Chest. 2003; 123: 1181-7.
  • 6. Bottini PV, Garlipp CR, Nogueira BF, Pompeo DB, Souza MI (2018). Performance evaluation of a new generation of automated analyzer for pleural and peritoneal fluids cytology. Clin Lab. 2018; 64: 1783- 6.
  • 7. Schonenberg H, Fassl H. Comparison of the coulter-counter-method and the counting-chamber to determine the cell count of the cerebrospinal fluid. Arztliche Forschung. 1970; 24: 118.
  • 8. Walker TJ, Nelson LD, Dunphy BW, Anderson DM, Kickler TS. Comparative Evaluation of the Iris iQ200 Body Fluid Module With Manual Hemacytometer Count. Am J Clin Pathol. 2009; 131: 333-8.
  • 9. Talstad I. Electronic counting of spinal fluid cells. Am J Clin Pathol. 1984; 81: 506-11.
  • 10. Aune MW, Becker JL, Brugnara C, et al. Automated flow cytometric analysis of blood cells in cerebrospinal fluid. Am J Clin Pathol. 2004; 121: 690-700.
  • 11. Takemura H, Ai T, Kimura K, et al. Evaluation of cell count and classification capabilities in body fluids using a fully automated Sysmex XN equipped with high-sensitive Analysis (hsA) mode and DI-60 hematology analyzer system. PloS one. 2018; 13: e0195923.
  • 12. Li PKT, Szeto CC, Piraino B, et al. Peritoneal dialysis-related infections recommendations: 2010 update. Perit Dial Int. 2010; 30: 393-423.
  • 13. Fleming C, Russcher H, Lindemans J, de Jonge R. Clinical relevance and contemporary methods for counting blood cells in body fluids suspected of inflammatory disease. Clin Chem Lab Med. 2015; 53: 1689-706.
APA Savci U, ŞAHİN M, Eser B, kayadibi h (2020). A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. , 278 - 285. 10.5798/dicletip.755702
Chicago Savci Unsal,ŞAHİN Mustafa Fethi,Eser Baris,kayadibi huseyin A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. (2020): 278 - 285. 10.5798/dicletip.755702
MLA Savci Unsal,ŞAHİN Mustafa Fethi,Eser Baris,kayadibi huseyin A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. , 2020, ss.278 - 285. 10.5798/dicletip.755702
AMA Savci U,ŞAHİN M,Eser B,kayadibi h A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. . 2020; 278 - 285. 10.5798/dicletip.755702
Vancouver Savci U,ŞAHİN M,Eser B,kayadibi h A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. . 2020; 278 - 285. 10.5798/dicletip.755702
IEEE Savci U,ŞAHİN M,Eser B,kayadibi h "A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids." , ss.278 - 285, 2020. 10.5798/dicletip.755702
ISNAD Savci, Unsal vd. "A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids". (2020), 278-285. https://doi.org/10.5798/dicletip.755702
APA Savci U, ŞAHİN M, Eser B, kayadibi h (2020). A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. Dicle Tıp Dergisi, 47(2), 278 - 285. 10.5798/dicletip.755702
Chicago Savci Unsal,ŞAHİN Mustafa Fethi,Eser Baris,kayadibi huseyin A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. Dicle Tıp Dergisi 47, no.2 (2020): 278 - 285. 10.5798/dicletip.755702
MLA Savci Unsal,ŞAHİN Mustafa Fethi,Eser Baris,kayadibi huseyin A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. Dicle Tıp Dergisi, vol.47, no.2, 2020, ss.278 - 285. 10.5798/dicletip.755702
AMA Savci U,ŞAHİN M,Eser B,kayadibi h A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. Dicle Tıp Dergisi. 2020; 47(2): 278 - 285. 10.5798/dicletip.755702
Vancouver Savci U,ŞAHİN M,Eser B,kayadibi h A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. Dicle Tıp Dergisi. 2020; 47(2): 278 - 285. 10.5798/dicletip.755702
IEEE Savci U,ŞAHİN M,Eser B,kayadibi h "A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids." Dicle Tıp Dergisi, 47, ss.278 - 285, 2020. 10.5798/dicletip.755702
ISNAD Savci, Unsal vd. "A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids". Dicle Tıp Dergisi 47/2 (2020), 278-285. https://doi.org/10.5798/dicletip.755702